B0SZ02 (SYE_CAUSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Caul_2786

Organism

Caulobacter sp. (strain K31) [Complete proteome] [HAMAP]

Taxonomic identifier

366602 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	Glutamate--tRNA ligase HAMAP-Rule MF_00022		PRO_0000367644
Regions
	Motif	18 – 28	11	"HIGH" region HAMAP-Rule MF_00022
	Motif	244 – 248	5	"KMSKS" region HAMAP-Rule MF_00022
Sites
	Binding site	247	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0SZ02 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: DE0F0108389F9089	FASTA	474	51,637
	10 20 30 40 50 60 MTNSPAPTTS GVVTRFAPSP TGFLHIGGAR TALFNWLYAR HTGGKFLVRV EDTDRERSTQ 70 80 90 100 110 120 AAVAAIFEGL DWLGMKSDEE VVFQHKKADR HREAVQQLLD TGRAYRCWMS VEDLALAREA 130 140 150 160 170 180 ARAGGPALRS PWRDAPPPND PTAPHVIRFK GPLDGTTLVD DLVKGPVTFD NAELDDLVLL 190 200 210 220 230 240 RADGAPTYNL AVVVDDHDMG VTHVIRGDDH LNNAARQTLI YQAMGWTLPA FGHIPLIHGP 250 260 270 280 290 300 DGAKLSKRHG AQAVGEFQDM GYLPEGLRNY LARLGWGHGD DEVFSDAQAI EWFDVKDVVK 310 320 330 340 350 360 APARLDWAKL NFINSQHLHV AEDGRLADLT LKALQAQGAP LPDDAPARLL ATVPQVKVGA 370 380 390 400 410 420 KTILELAEHC AFALKVRPLA LEEKTRNQLT DETVDRLKRL RAQLGAVPVW GLSELEVLLK 430 440 450 460 470 SFAESEGVGF GKFGPSLRGI LTGGSQAPDL NKIMTALGRE ESLGRLDDAL ASRA

References

[1]

"Complete sequence of chromosome of Caulobacter sp. K31." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Thompson L.S., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Stephens C., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000927 Genomic DNA. Translation: ABZ71913.1.
RefSeq	YP_001684411.1. NC_010338.1.
3D structure databases
ProteinModelPortal	B0SZ02.
ModBase	Search...
Protein-protein interaction databases
STRING	366602.Caul_2786.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABZ71913; ABZ71913; Caul_2786.
GeneID	5900241.
KEGG	cak:Caul_2786.
PATRIC	21318648. VBICauSp18104_3181.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000252722.
KO	K01885.
OMA	HLANTID.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	CSP366602:GH0Y-2784-MONOMER.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_B. Glu_tRNA_synth_B.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_CAUSK

Accession

Primary (citable) accession number: B0SZ02

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program