Reviewed, UniProtKB/Swiss-Prot B0SYZ8 (LEXA_CAUSK)

Names and origin

Protein names

Recommended name:
    LexA repressor
    EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	Caul_2782

Organism

Caulobacter sp. (strain K31) [Complete proteome] [HAMAP]

Taxonomic identifier

366602 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity.
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	LexA repressor HAMAP MF_00015		PRO_1000074050
Regions
	DNA binding	26 – 46	21	H-T-H motif By similarity
Sites
	Active site	156	1	For autocatalytic cleavage activity By similarity
	Active site	193	1	For autocatalytic cleavage activity By similarity
	Site	121 – 122	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0SYZ8-1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 5686CC84E3A4BF22	FASTA	235	25,650
	10 20 30 40 50 60 MLTRKQHELL MFIHERIKES GVSPSFDEMK EALDLASKSG IHRLITALEE RGFIRRLAHR 70 80 90 100 110 120 ARALEVVKLP QQATTAAPPK GRGAFRPQVL EGGGQAPTTS AQPQMAADNS RELPILGRIA 130 140 150 160 170 180 AGTPIDAIQH ERERLPVPES MLGAGEHYVL EVQGDSMIEA GILDGDYVII KKGDTANSGE 190 200 210 220 230 IVVALVGEEA TLKRLRKKGG SIALEAANPK YETRIFGPDQ VEVQGKLVGL IRRYH

References

[1]

"Complete sequence of chromosome of Caulobacter sp. K31." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Thompson L.S., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Stephens C., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000927 Genomic DNA. Translation: ABZ71909.1.
RefSeq	YP_001684407.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S24.001.
Genome annotation databases
GeneID	5900237.
GenomeReviews	Gene locus Caul_2782 in contig CP000927_GR.
KEGG	cak:Caul_2782.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B0SYZ8. AILTFIR.
Family and domain databases
HAMAP	MF_00015. [Tree]
InterPro	IPR006199. LexA_DNA_bd. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_CAUSK

Accession

Primary (citable) accession number: B0SYZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	June 16, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)