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Protein

Thiazole synthase

Gene

thiG

Organism
Caulobacter sp. (strain K31)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei111Schiff-base intermediate with DXPUniRule annotation1
Binding sitei172DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:Caul_2568
OrganismiCaulobacter sp. (strain K31)
Taxonomic identifieri366602 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
Proteomesi
  • UP000001316 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000808671 – 268Thiazole synthaseAdd BLAST268

Proteomic databases

PRIDEiB0SXB0

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi366602.Caul_2568

Structurei

3D structure databases

ProteinModelPortaliB0SXB0
SMRiB0SXB0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni198 – 199DXP bindingUniRule annotation2
Regioni220 – 221DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8 Bacteria
COG2022 LUCA
HOGENOMiHOG000248049
KOiK03149
OMAiAQYPSPA
OrthoDBiPOG091H01K9

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

B0SXB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAHVPPESV SAAEDSWTVA GRTFRSRLIV GTGKYKDYAT NAAAAKAAGA
60 70 80 90 100
EIVTVAVRRV NLTDPTQPLL IDYVKPTEFT YLPNTAGCFT GEDAVRTLRL
110 120 130 140 150
AREAGGWDLV KLEVLSDPKT LYPDMEETLR SLKLLVSEGF QVMVYCSDDP
160 170 180 190 200
VYARKLEEAG AVAIMPLGAP IGSGLGIQNR VNLRIIVENA GVPVLVDAGV
210 220 230 240 250
GTASDAAIGM ELGCDAILMN TAIAEAKDPI RMARAMKHAV IAGREAYLAG
260
RMQKRLYADP SSPLGGLI
Length:268
Mass (Da):28,533
Last modified:April 8, 2008 - v1
Checksum:i47306C628C260BC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000927 Genomic DNA Translation: ABZ71695.1
RefSeqiWP_012286598.1, NC_010338.1

Genome annotation databases

EnsemblBacteriaiABZ71695; ABZ71695; Caul_2568
KEGGicak:Caul_2568

Similar proteinsi

Entry informationi

Entry nameiTHIG_CAUSK
AccessioniPrimary (citable) accession number: B0SXB0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: May 23, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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