ID   B0SWV2_CAUSK            Unreviewed;       462 AA.
AC   B0SWV2;
DT   08-APR-2008, integrated into UniProtKB/TrEMBL.
DT   08-APR-2008, sequence version 1.
DT   27-MAR-2024, entry version 93.
DE   SubName: Full=Mannosyl-oligosaccharide 1,2-alpha-mannosidase {ECO:0000313|EMBL:ABZ73160.1};
DE            EC=3.2.1.113 {ECO:0000313|EMBL:ABZ73160.1};
DE   Flags: Precursor;
GN   OrderedLocusNames=Caul_4035 {ECO:0000313|EMBL:ABZ73160.1};
OS   Caulobacter sp. (strain K31).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
OC   Caulobacteraceae; Caulobacter.
OX   NCBI_TaxID=366602 {ECO:0000313|EMBL:ABZ73160.1};
RN   [1] {ECO:0000313|EMBL:ABZ73160.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K31 {ECO:0000313|EMBL:ABZ73160.1};
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Bruce D., Goodwin L., Thompson L.S., Brettin T.,
RA   Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Kim E., Stephens C., Richardson P.;
RT   "Complete sequence of chromosome of Caulobacter sp. K31.";
RL   Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0007829|PDB:4AYO, ECO:0007829|PDB:4AYP}
RP   X-RAY CRYSTALLOGRAPHY (0.85 ANGSTROMS) OF 27-462 IN COMPLEX WITH CA(2+).
RX   PubMed=23012075; DOI=10.1002/anie.201205338;
RA   Thompson A.J., Dabin J., Iglesias-Fernandez J., Ardevol A., Dinev Z.,
RA   Williams S.J., Bande O., Siriwardena A., Moreland C., Hu T.C., Smith D.K.,
RA   Gilbert H.J., Rovira C., Davies G.J.;
RT   "The reaction coordinate of a bacterial GH47 alpha-mannosidase: a combined
RT   quantum mechanical and structural approach.";
RL   Angew. Chem. Int. Ed. 51:10997-11001(2012).
RN   [3] {ECO:0007829|PDB:5MEH}
RP   X-RAY CRYSTALLOGRAPHY (0.95 ANGSTROMS) OF 27-462 IN COMPLEX WITH CA(2+).
RX   PubMed=28256791; DOI=10.1002/cbic.201700080;
RA   van Rijssel E.R., Janssen A.P.A., Males A., Davies G.J.,
RA   van der Marel G.A., Overkleeft H.S., Codee J.D.C.;
RT   "Conformational Behaviour of Azasugars Based on Mannuronic Acid.";
RL   ChemBioChem 18:1297-1304(2017).
RN   [4] {ECO:0007829|PDB:5NE5}
RP   X-RAY CRYSTALLOGRAPHY (1.05 ANGSTROMS) OF 27-462 IN COMPLEX WITH CA(2+).
RX   PubMed=28493500; DOI=10.1002/cbic.201700166;
RA   Males A., Raich L., Williams S.J., Rovira C., Davies G.J.;
RT   "Conformational Analysis of the Mannosidase Inhibitor Kifunensine: A
RT   Quantum Mechanical and Structural Approach.";
RL   ChemBioChem 18:1496-1501(2017).
RN   [5] {ECO:0007829|PDB:8B5M}
RP   X-RAY CRYSTALLOGRAPHY (0.97 ANGSTROMS) OF 27-462 IN COMPLEX WITH CA(2+).
RA   Males A., Davies G.J., Artola M., Overkleeft H.S.;
RT   "GH47 and Cyclosulfamidate.";
RL   Submitted (SEP-2022) to the PDB data bank.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|ARBA:ARBA00001913};
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC       {ECO:0000256|ARBA:ARBA00004922}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 47 family.
CC       {ECO:0000256|ARBA:ARBA00007658}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000927; ABZ73160.1; -; Genomic_DNA.
DR   PDB; 4AYO; X-ray; 0.85 A; A=27-462.
DR   PDB; 4AYP; X-ray; 0.85 A; A=27-462.
DR   PDB; 4AYQ; X-ray; 1.10 A; A=27-462.
DR   PDB; 4AYR; X-ray; 1.10 A; A=27-462.
DR   PDB; 5MEH; X-ray; 0.95 A; A=27-462.
DR   PDB; 5NE5; X-ray; 1.05 A; A=27-462.
DR   PDB; 8B5M; X-ray; 0.97 A; A=27-462.
DR   PDBsum; 4AYO; -.
DR   PDBsum; 4AYP; -.
DR   PDBsum; 4AYQ; -.
DR   PDBsum; 4AYR; -.
DR   PDBsum; 5MEH; -.
DR   PDBsum; 5NE5; -.
DR   AlphaFoldDB; B0SWV2; -.
DR   SMR; B0SWV2; -.
DR   STRING; 366602.Caul_4035; -.
DR   CAZy; GH47; Glycoside Hydrolase Family 47.
DR   KEGG; cak:Caul_4035; -.
DR   eggNOG; ENOG502Z9K5; Bacteria.
DR   HOGENOM; CLU_003818_5_6_5; -.
DR   OrthoDB; 1110235at2; -.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0004571; F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001382; Glyco_hydro_47.
DR   InterPro; IPR036026; Seven-hairpin_glycosidases.
DR   InterPro; IPR006311; TAT_signal.
DR   PANTHER; PTHR11742:SF55; ENDOPLASMIC RETICULUM MANNOSYL-OLIGOSACCHARIDE 1,2-ALPHA-MANNOSIDASE; 1.
DR   PANTHER; PTHR11742; MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED; 1.
DR   Pfam; PF01532; Glyco_hydro_47; 1.
DR   PRINTS; PR00747; GLYHDRLASE47.
DR   SUPFAM; SSF48225; Seven-hairpin glycosidases; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:4AYO, ECO:0007829|PDB:4AYP};
KW   Calcium {ECO:0000256|ARBA:ARBA00022837, ECO:0007829|PDB:4AYO};
KW   Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW   Glycosidase {ECO:0000313|EMBL:ABZ73160.1};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:ABZ73160.1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0007829|PDB:4AYO};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           26..462
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5002755638"
FT   BINDING         253
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0007829|PDB:4AYO, ECO:0007829|PDB:4AYP"
FT   BINDING         451
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:4AYO, ECO:0007829|PDB:4AYP"
SQ   SEQUENCE   462 AA;  51676 MW;  D20ECCB0C33D4ECC CRC64;
     MTASHLSRRH ALALVASAAA APAFAAETTP EDWKALAADV RSEFQWAWQG YVAKAWGKDE
     INPVSGTSRS FFIEGHDLGL SLVEALDTLW IMGLDAEFQA GVDWVKANLS FDVDGNAQVF
     ETNIRLVGGL LSAHLASGDP VLLAKARDLA DRLAKAFEAS PHGLPWRYVN LRTGAVSDPE
     TNLAEIGTYL SEFGVLSQLT GERKYFDMAK RAMRHTLDRR SKIGLMAANI HAMTGAFTSR
     NASIDVYADS FYEYLWDAWA LFGDEDCKRW AVECVDAQLA HQAKRYDGRL WFPMVDFETG
     AVTGTAQSEL AAYYAGLLGQ VGRKAQGDDY LASFTYLQAT FGVIPESIDV TTGQPRRKHT
     GLRPEYPDAC LNLWLIDRDP RYRRLAAIHY REMKATSRAA FGYTALKDIT TRPMTQDDNC
     PGYWWSEQMK YYYLLFSDTP RIDYGQLQLS TEANVLRGFR KV
//