B0SWQ6 (SYD_CAUSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	Caul_2557

Organism

Caulobacter sp. (strain K31) [Complete proteome] [HAMAP]

Taxonomic identifier

366602 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 609	609	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000074694

Sequences

	Sequence		Length	Mass (Da)
	B0SWQ6 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 82ECE8C71554B9F0	FASTA	609	68,147
	10 20 30 40 50 60 MHAYRTHTCG ALRASDTGAS VRVSGWIHRK RDHGGLVFID LRDHYGLTQL VLHPETPGFD 70 80 90 100 110 120 VVERLRAESV IKIDGEVVAR DAAAVNPNLP TGEIEIRVSA VEVLSEAAEL PLPVFGEPDY 130 140 150 160 170 180 PEEIRLKHRY LDLRRETLHK NIVLRSRVIQ SIRSRMFAQG FNEFQTPILT ASSPEGARDF 190 200 210 220 230 240 LVPSRLHPGK FYALPQAPQQ FKQLLMVSGF DRYFQIAPCF RDEDLRADRS LEFYQLDVEM 250 260 270 280 290 300 SFVTQEDVFA AIEPVMHGVF EEFSAGKPVS PIDGVHTFTN DFGATLEHRG FERLTYAQSM 310 320 330 340 350 360 AWYGSDKPDL RNPIKMQDVS EHFRDGGFGL FAKILGADPK NRVWAIPAPT GGSRAFCDRM 370 380 390 400 410 420 NSWAQGEGQP GLGYAFFSKD QNGWGGPIAK NLGEGFQPIA DQLGLTHDDA VFFVAGDPAV 430 440 450 460 470 480 FAKFAGLART RVGTELKLVD EEQFKFCWIV DFPMFEWNED EKKVDFSHNP FSMPQGGLEA 490 500 510 520 530 540 LETQDPLTIR AYQYDIVCNG YELCSGAIRN HKPEIMLKAF EVAGYGAEVV EEQFGGMLNA 550 560 570 580 590 600 FRYGAPPHGG LAPGIDRIVM LLAEQVAIRE VIAFPLNQQG QDLLMNAPAE AQDRQYKELY IRSAPPIKV

References

[1]

"Complete sequence of chromosome of Caulobacter sp. K31." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Thompson L.S., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Stephens C., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000927 Genomic DNA. Translation: ABZ71684.1.
RefSeq	YP_001684182.1. NC_010338.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B0SWQ6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5900012.
GenomeReviews	Gene locus Caul_2557 in contig CP000927_GR.
KEGG	cak:Caul_2557.
PATRIC	21318180. VBICauSp18104_2948.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	YQLDVEM.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	CSP78:CAUL_2557-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_CAUSK

Accession

Primary (citable) accession number: B0SWQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program