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B0STE5

- SYI_LEPBP

UniProt

B0STE5 - SYI_LEPBP

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Protein
Isoleucine--tRNA ligase
Gene
ileS, LEPBI_I2290
Organism
Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei557 – 5571Aminoacyl-adenylate By similarity
Binding sitei601 – 6011ATP By similarity
Metal bindingi887 – 8871Zinc By similarity
Metal bindingi890 – 8901Zinc By similarity
Metal bindingi902 – 9021Zinc By similarity
Metal bindingi905 – 9051Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLBIF456481:GCM0-2270-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:LEPBI_I2290
OrganismiLeptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris)
Taxonomic identifieri456481 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
ProteomesiUP000001847: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 915915Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189178Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi456481.LEPBI_I2290.

Structurei

3D structure databases

ProteinModelPortaliB0STE5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 7411"HIGH" regionUniRule annotation
Add
BLAST
Motifi598 – 6025"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0STE5-1 [UniParc]FASTAAdd to Basket

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MAKPETENPY SKTVLLPETS FPMKADLAKR EPGQIKVWKD QKVFLNMKEI    50
RKSKPSFVLH DGPPYANGNF HVGHSLNKIL KDIIIKSKTL SGYQTDMIPG 100
WDCHGLPIEV QVLKNLGKEA RNTGPSELRK KCREYAAEFV GKQGEDLNRF 150
LCFWDENNKY LTMAPEFEAR IVEVFGSLFA KGYIYKGKKP VYWCIDLATA 200
HAEAEIEYQN HVSPSIYVKF AVKGETDTHC LIWTTTPWTL PANLAICFNE 250
ELPYSLFQSD AHGRLILADG LKEAVEQKTG ITLTKIKSLS NADLKQMVFL 300
HPFLDRESIP LFGNHVTLDA GTGCVHTAPG HGTDDYRVGT AAGLPTLSPV 350
DDYGRYTDEF EMMKGIKIWD ANPKIVELLR EKNALVHFSE FTHSYPHSWR 400
SKKPLIFRAT PQWFFSIDHN GLRDESLKAI DKVQWIPDWG ITRIRSMVES 450
RPDWCLSRQR NWGVPIPSFT CKSCGLTHLD DKTIQHFIQI VKKEGIEVWY 500
EKEAKDLLPA DTKCSNCGSE DLKQDKDILD VWFDSGVSSF AVFGDSIGKE 550
PADLYLEGSD QHRGWFQSSL WPSMAIRKTP PYKSVLTHGY VLDEKGHAMS 600
KSLGNVINPT TDIINQYGAD ILRLWVSTQD FRDDVKIGKD SIKTVSEAYR 650
KIRNTFRYLL GNTSAETLTW NLKKEELDTI DKYYLHKLAK LNDEVKKLYD 700
TYQFHQVYHK ILGFCTVDLS QDYFEIIRDR MYCDAKESKT RRSSEYTLAV 750
ILEVLSKLLA PILSFTTEEV WTSFGKKDSV FYSDFSDLTE WLDESLESKM 800
KPVFETKEDV QKALEEARKL GKLGKSLEAE VVIDGKKDSL PFSSEELALF 850
FVVSHVHFET NGIQEVFSEW KGESGSIQIR KPKHFECPRC WRHVSETEGK 900
LCKRCDEVVS KLSPN 915
Length:915
Mass (Da):104,704
Last modified:April 8, 2008 - v1
Checksum:iF31CD5A61F29C54F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000786 Genomic DNA. Translation: ABZ98385.1.
RefSeqiYP_001839661.1. NC_010602.1.

Genome annotation databases

EnsemblBacteriaiABZ98385; ABZ98385; LEPBI_I2290.
GeneIDi6222361.
KEGGilbi:LEPBI_I2290.
PATRICi22351346. VBILepBif123590_2299.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000786 Genomic DNA. Translation: ABZ98385.1 .
RefSeqi YP_001839661.1. NC_010602.1.

3D structure databases

ProteinModelPortali B0STE5.
ModBasei Search...

Protein-protein interaction databases

STRINGi 456481.LEPBI_I2290.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABZ98385 ; ABZ98385 ; LEPBI_I2290 .
GeneIDi 6222361.
KEGGi lbi:LEPBI_I2290.
PATRICi 22351346. VBILepBif123590_2299.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LBIF456481:GCM0-2270-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis."
    Picardeau M., Bulach D.M., Bouchier C., Zuerner R.L., Zidane N., Wilson P.J., Creno S., Kuczek E.S., Bommezzadri S., Davis J.C., McGrath A., Johnson M.J., Boursaux-Eude C., Seemann T., Rouy Z., Coppel R.L., Rood J.I., Lajus A.
    , Davies J.K., Medigue C., Adler B.
    PLoS ONE 3:E1607-E1607(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Patoc 1 / ATCC 23582 / Paris.

Entry informationi

Entry nameiSYI_LEPBP
AccessioniPrimary (citable) accession number: B0STE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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