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B0SS77 (DDL_LEPBP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:LEPBI_I1863
OrganismLeptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) [Complete proteome] [HAMAP]
Taxonomic identifier456481 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000091191

Regions

Domain140 – 345206ATP-grasp
Nucleotide binding169 – 22456ATP By similarity

Sites

Metal binding3001Magnesium or manganese 1 By similarity
Metal binding3121Magnesium or manganese 1 By similarity
Metal binding3121Magnesium or manganese 2 By similarity
Metal binding3141Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B0SS77 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 62516629678BF0D9

FASTA34938,026
        10         20         30         40         50         60 
MSKIKIALLF GGISGEHIIS VRSSAFIFAT IDREKYDVCP VYINPNGKFW IPTVSEPIYP 

        70         80         90        100        110        120 
DPSGKTEIEF LQEFNKANAI VSPSEPADIS QMGFLSAFLG LHGGAGEDGR IQGFLDTLGI 

       130        140        150        160        170        180 
PHTGSGVLAS SLAMDKYRAN ILFEAMGIPV APFLELEKGK TDPRKTLLNL SFSYPVFIKP 

       190        200        210        220        230        240 
TLGGSSVNTG MAKTAEEAMT LVDKIFVTDD RVLVQKLVSG TEVSIGVLEK PEGKKRNPFP 

       250        260        270        280        290        300 
LVPTEIRPKS EFFDFEAKYT KGASEEITPA PVGDEVTKTL QEYTLRCHEI LGCKGYSRTD 

       310        320        330        340 
FIISDGVPYV LETNTLPGMT GTSLIPQQAK ALGINMKDVF TWLLEISLS 

« Hide

References

[1]"Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis."
Picardeau M., Bulach D.M., Bouchier C., Zuerner R.L., Zidane N., Wilson P.J., Creno S., Kuczek E.S., Bommezzadri S., Davis J.C., McGrath A., Johnson M.J., Boursaux-Eude C., Seemann T., Rouy Z., Coppel R.L., Rood J.I., Lajus A. expand/collapse author list , Davies J.K., Medigue C., Adler B.
PLoS ONE 3:E1607-E1607(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Patoc 1 / ATCC 23582 / Paris.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000786 Genomic DNA. Translation: ABZ97967.1.
RefSeqYP_001839243.1. NC_010602.1.

3D structure databases

ProteinModelPortalB0SS77.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING456481.LEPBI_I1863.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ97967; ABZ97967; LEPBI_I1863.
GeneID6221625.
KEGGlbi:LEPBI_I1863.
PATRIC22350550. VBILepBif123590_1906.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMARSAYGIY.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycLBIF456481:GCM0-1847-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_LEPBP
AccessionPrimary (citable) accession number: B0SS77
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways