true2009-09-222024-03-2794LIPA_LEPBPGenome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis.Picardeau M.Bulach D.M.Bouchier C.Zuerner R.L.Zidane N.Wilson P.J.Creno S.Kuczek E.S.Bommezzadri S.Davis J.C.McGrath A.Johnson M.J.Boursaux-Eude C.Seemann T.Rouy Z.Coppel R.L.Rood J.I.Lajus A.Davies J.K.Medigue C.Adler B.doi:10.1371/journal.pone.00016072008PLoS ONE3E1607NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]Patoc 1 / ATCC 23582 / ParisRadical_SAMAldolase class ILipoyl_synthAldolase_TIMElp3/MiaA/NifB-like_rSAMLipoyl_synthrSAMlipALIPOYL SYNTHASELIPOYL SYNTHASE, MITOCHONDRIALRadical_SAMLipoyl_synthlipoyl_synthaselipoyl_synthase_likeElp3Radical SAM enzymesRADICAL_SAMLipoyl synthase2.8.1.8Lip-synLSLipoate synthaseLipoic acid synthaseSulfur insertion protein LipAlipALEPBI_I1462Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.Belongs to the radical SAM superfamily. Lipoyl synthase family.Lipoyl synthase344691306Radical SAM core64278525763788285289124Fe-4S-S-AdoMet2008-04-081344696d5adae96317d682e304467e5ec2a519MNPLKKKPRSKNLNPTVPLPDWMKVRVSFPTDSDALSVVRAEVESKELHTVCESASCPNLNHCWNRKTATYMLAGDICTRRCQYCDVAFGKPKPLDSLEPERVARSVQSLGLRHVVLTAVNRDDLKDGGASHFAETITKIKTYHKDCTIEVLIPDFKAKEDSLQILYAAKPNIINHNIETVESLFPTITPQKNYKRSLEVLAHIANHGFLTKSGIILGLGETDEDVNQCLMDLFAHGVRMLTIGQYLQPGPTHYPVQSFVRPETFVMWKETAYKIGFKTVASGPLVRSSYHADEYFHEESQILPTEtruetrue