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Protein

Catalase-peroxidase

Gene

katG

Organism
Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotation

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei106 – 1061Transition state stabilizerUniRule annotation
Active sitei110 – 1101Proton acceptorUniRule annotation
Metal bindingi272 – 2721Iron (heme axial ligand)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciLBIF456481:GCM0-2474-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CPUniRule annotation
Alternative name(s):
Peroxidase/catalaseUniRule annotation
Gene namesi
Name:katGUniRule annotation
Ordered Locus Names:LEPBI_I2495
OrganismiLeptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris)
Taxonomic identifieri456481 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesLeptospiralesLeptospiraceaeLeptospira
Proteomesi
  • UP000001847 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121UniRule annotationAdd
BLAST
Chaini22 – 741720Catalase-peroxidasePRO_0000354825Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki109 ↔ 231Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-257)UniRule annotation
Cross-linki231 ↔ 257Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-109)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Proteomic databases

PRIDEiB0SLJ6.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi456481.LEPBI_I2495.

Structurei

3D structure databases

ProteinModelPortaliB0SLJ6.
SMRiB0SLJ6. Positions 35-740.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiVVWTPTP.
OrthoDBiEOG6RRKKM.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0SLJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNFRRFTIA LLVLFLGPIG AADTKETPGM DRQNTSNQFW WPERLDLAPL
60 70 80 90 100
RQHGSESNPL GRQFHYAKEF KELDIQTLKE EIKTVMKTSQ DWWPADYGHY
110 120 130 140 150
GPFFIRMAWH SAGTYRISDG RGGAGGGQQR FEPLNSWPDN ANLDKARRLL
160 170 180 190 200
WPIKKKYGKK ISWADLMVLT GNVALESMGF KTYGFAGGRT DDWEADLVYW
210 220 230 240 250
GPEKKFLEDQ RYKGNRELKN PLAAVQMGLI YVNPEGPNGN PDPLAAAKDI
260 270 280 290 300
RETFGRMAMN DEETVALIAG GHTFGKAHGK SDPSKHVGKE PAAAGLEEQG
310 320 330 340 350
FGWKNNYKKG NAEDTITSGL EGAWTANPTK WTTQYLNNLF GFEWVQTKSP
360 370 380 390 400
AGAIQWVPKD GAGANMVPDA HDKSLRHAPI MFTTDLALKF DPSYKVIAKR
410 420 430 440 450
FQENPKEFEL AFAKAWFKLT HRDMGPLTRY IGKDLPKEPL IWQDPVPAVN
460 470 480 490 500
HKLVGPKEIE SLKGKILKSG LSVPQLVRTA WASAASFRST DMRGGANGAR
510 520 530 540 550
IRLSPQKNWP VNDPDELSKV LKKLEQIQEE FNKSGNKISL ADLIVLAGNA
560 570 580 590 600
AIEEAAKKAG VKVTVPFTPG RTDATIEQTD EYSFSVLEPK ADAFRNYYGP
610 620 630 640 650
GNLMSPTEML VDRANMLSLS IPEMTVLLGG MRSLDANAGK SKHGILTTKP
660 670 680 690 700
GVLSNDFFVN LLDMSTKWQK SEQTEGLYEG LDRKTGSKRW TATSVDLIFG
710 720 730 740
SHSELRAVAE VYASDDAKEK FVKDFVSAWN KVMMLDRFDV K
Length:741
Mass (Da):82,737
Last modified:April 8, 2008 - v1
Checksum:i74E8023F67322DC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000786 Genomic DNA. Translation: ABZ98582.1.
RefSeqiWP_012389443.1. NC_010602.1.

Genome annotation databases

EnsemblBacteriaiABZ98582; ABZ98582; LEPBI_I2495.
KEGGilbi:LEPBI_I2495.
PATRICi22351793. VBILepBif123590_2518.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000786 Genomic DNA. Translation: ABZ98582.1.
RefSeqiWP_012389443.1. NC_010602.1.

3D structure databases

ProteinModelPortaliB0SLJ6.
SMRiB0SLJ6. Positions 35-740.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi456481.LEPBI_I2495.

Proteomic databases

PRIDEiB0SLJ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABZ98582; ABZ98582; LEPBI_I2495.
KEGGilbi:LEPBI_I2495.
PATRICi22351793. VBILepBif123590_2518.

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiVVWTPTP.
OrthoDBiEOG6RRKKM.

Enzyme and pathway databases

BioCyciLBIF456481:GCM0-2474-MONOMER.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis."
    Picardeau M., Bulach D.M., Bouchier C., Zuerner R.L., Zidane N., Wilson P.J., Creno S., Kuczek E.S., Bommezzadri S., Davis J.C., McGrath A., Johnson M.J., Boursaux-Eude C., Seemann T., Rouy Z., Coppel R.L., Rood J.I., Lajus A.
    , Davies J.K., Medigue C., Adler B.
    PLoS ONE 3:E1607-E1607(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Patoc 1 / ATCC 23582 / Paris.

Entry informationi

Entry nameiKATG_LEPBP
AccessioniPrimary (citable) accession number: B0SLJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: April 8, 2008
Last modified: December 9, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.