B0SKG9 (ISPE_LEPBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	LEPBI_I0238

Organism

Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) [Complete proteome] [HAMAP]

Taxonomic identifier

456481 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000092094
Regions
	Nucleotide binding	113 – 123	11	ATP Potential
Sites
	Active site	11	1	By similarity
	Active site	153	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0SKG9 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 835BFF4089AF3A8B	FASTA	306	34,276
	10 20 30 40 50 60 MVLLQTIAHA KINIGLMIPY KREDGLHEIR SVFVPIDFGD PMEIQIKSLP KGIVSRFVFH 70 80 90 100 110 120 STNHLQGYRH SLFEAVSERG DLSQNILTKT FHKLKPYFQE ALEIKVQIQK HLPPEGGIGG 130 140 150 160 170 180 GSSNAGVLLQ HLFPYTNLSR GEQIQFAKSI GADVPFFLQS SACFVTGIGE VMEPIRLAKG 190 200 210 220 230 240 FGILAIPPFG LSTGSMYASL QKSLQKPYGS EVWKSLTEDL IRSLHVGDWV YLQNRLENEF 250 260 270 280 290 300 EKIAFQTQPL LKELKLGFFE SGAIFASLSG SGSCLYGIYP TEEKRNEALP NVSLRFPKME FRTFSF

References

[1]

"Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis." Picardeau M., Bulach D.M., Bouchier C., Zuerner R.L., Zidane N., Wilson P.J., Creno S., Kuczek E.S., Bommezzadri S., Davis J.C., McGrath A., Johnson M.J., Boursaux-Eude C., Seemann T., Rouy Z., Coppel R.L., Rood J.I., Lajus A. , Davies J.K., Medigue C., Adler B. PLoS ONE 3:E1607-E1607(2008) [PubMed: 18270594] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Patoc 1 / ATCC 23582 / Paris.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000786 Genomic DNA. Translation: ABZ96383.1.
RefSeq	YP_001837659.1. NC_010602.1.
3D structure databases
ProteinModelPortal	B0SKG9.
ModBase	Search...
Protein-protein interaction databases
STRING	B0SKG9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6222904.
GenomeReviews	Gene locus LEPBI_I0238 in contig CP000786_GR.
KEGG	lbi:LEPBI_I0238.
PATRIC	22347172. VBILepBif123590_0240.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	SSTKEMY.
ProtClustDB	PRK14613.
Enzyme and pathway databases
BioCyc	LBIF456481:LEPBI_I0238-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_LEPBP

Accession

Primary (citable) accession number: B0SKG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program