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Protein

Pyridoxine 5'-phosphate synthase

Gene

pdxJ

Organism
Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2 H2O.UniRule annotation

Pathwayi: pyridoxine 5'-phosphate biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Phosphoserine aminotransferase (serC)
  4. no protein annotated in this organism
  5. Pyridoxine 5'-phosphate synthase (pdxJ)
This subpathway is part of the pathway pyridoxine 5'-phosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate, the pathway pyridoxine 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73-amino-2-oxopropyl phosphateUniRule annotation1
Binding sitei183-amino-2-oxopropyl phosphateUniRule annotation1
Active sitei43Proton acceptorUniRule annotation1
Binding sitei451-deoxy-D-xylulose 5-phosphateUniRule annotation1
Binding sitei501-deoxy-D-xylulose 5-phosphateUniRule annotation1
Active sitei83Proton acceptorUniRule annotation1
Binding sitei1131-deoxy-D-xylulose 5-phosphateUniRule annotation1
Sitei166Transition state stabilizerUniRule annotation1
Active sitei208Proton donorUniRule annotation1
Binding sitei2093-amino-2-oxopropyl phosphate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPyridoxine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00244; UER00313

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxine 5'-phosphate synthaseUniRule annotation (EC:2.6.99.2UniRule annotation)
Short name:
PNP synthaseUniRule annotation
Gene namesi
Name:pdxJUniRule annotation
Ordered Locus Names:LEPBI_I0476
OrganismiLeptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris)
Taxonomic identifieri456481 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesLeptospiralesLeptospiraceaeLeptospira
Proteomesi
  • UP000001847 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001148151 – 260Pyridoxine 5'-phosphate synthaseAdd BLAST260

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi456481.LEPBI_I0476

Structurei

3D structure databases

ProteinModelPortaliB0SJ25
SMRiB0SJ25
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni230 – 2313-amino-2-oxopropyl phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PNP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CSZ Bacteria
COG0854 LUCA
HOGENOMiHOG000258095
KOiK03474
OMAiTSNAGWD
OrthoDBiPOG091H03D1

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00279 PdxJ, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR004569 PyrdxlP_synth_PdxJ
IPR036130 Pyridoxine-5'_phos_synth
PANTHERiPTHR30456 PTHR30456, 1 hit
PfamiView protein in Pfam
PF03740 PdxJ, 1 hit
SUPFAMiSSF63892 SSF63892, 1 hit
TIGRFAMsiTIGR00559 pdxJ, 1 hit

Sequencei

Sequence statusi: Complete.

B0SJ25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQLSVNVNK IATLRNSRGG SLPSVLKLSE LILDSGAHGI TVHPRSDERH
60 70 80 90 100
ITKQDVFELQ EFLRTYNEKI TKLGISKKEY NIEGEPSERF LDLVLKAKPD
110 120 130 140 150
QATLVPVKPG EITSDHGFQF ADQKTFSTLK PIVEAIRKEG IRVSLFMETD
160 170 180 190 200
FTFYDQVVAL GAERIELYTG PFAHAFDLSE EKGKEIFTDY QRAAIEANKL
210 220 230 240 250
GLAVNAGHDL DTNNLRVFAK LPYLKEVSIG HRLMAQSLVD GLETTVKSYL
260
KVLSLGNETE
Length:260
Mass (Da):29,082
Last modified:April 8, 2008 - v1
Checksum:iF865749A1E31FBD6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000786 Genomic DNA Translation: ABZ96614.1
RefSeqiWP_012387501.1, NC_010602.1

Genome annotation databases

EnsemblBacteriaiABZ96614; ABZ96614; LEPBI_I0476
GeneIDi35830743
KEGGilbi:LEPBI_I0476

Similar proteinsi

Entry informationi

Entry nameiPDXJ_LEPBP
AccessioniPrimary (citable) accession number: B0SJ25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 8, 2008
Last modified: April 25, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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