Reviewed, UniProtKB/Swiss-Prot B0SFG5 (MTNC_LEPBA)

Names and origin

Protein names

Recommended name:
    Enolase-phosphatase E1
    EC=3.1.3.77
Alternative name(s):
    2,3-diketo-5-methylthio-1-phosphopentane phosphatase

Gene names

Name:	mtnC
Ordered Locus Names:	LBF_2858

Organism

Leptospira biflexa serovar Patoc (strain Patoc 1 / Ames) [Complete proteome] [HAMAP]

Taxonomic identifier

355278 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) By similarity.
Catalytic activity	5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate. HAMAP MF_01681
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. HAMAP MF_01681 Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process from S-adenosylmethionine Inferred from electronic annotation. Source: HAMAP methionine salvage Inferred from electronic annotation. Source: InterPro
Molecular function	2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity Inferred from electronic annotation. Source: HAMAP 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity Inferred from electronic annotation. Source: HAMAP acireductone synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	Enolase-phosphatase E1 HAMAP MF_01681		PRO_0000357375

Sequences

	Sequence		Length	Mass (Da)
	B0SFG5-1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 6C153DA24CA9EF49	FASTA	247	28,335
	10 20 30 40 50 60 MQIKHNLLDI EGTTAPIAFV HQILFPYATK NIHRFLKEYQ LTELQWKEVQ TEFQKDTSSG 70 80 90 100 110 120 DPLFIEKFRI KNVPSGLIVN EVPNTLSKDM VSVYFEYLIE KDRKFGPLKE IQGKIWKEGY 130 140 150 160 170 180 ESGEIKSTVF DDVPKFLNDA IQSGIQNHVY SSGSVEAQHL IYQYSVLGDL RQYFTMYFDT 190 200 210 220 230 240 AVGGKREKTS YERIASTLAV SPSEIRFFTD IVEEAEAANA TGMDVVILNR PGNLAQKPHP FPVWEHF

References

[1]

"Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis." Picardeau M., Bulach D.M., Bouchier C., Zuerner R.L., Zidane N., Wilson P.J., Creno S., Kuczek E.S., Bommezzadri S., Davis J.C., McGrath A., Johnson M.J., Boursaux-Eude C., Seemann T., Rouy Z., Coppel R.L., Rood J.I., Lajus A. , Davies J.K., Medigue C., Adler B. PLoS ONE 3:E1607-E1607(2008) [PubMed: 18270594] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000777 Genomic DNA. Translation: ABZ95333.1.
RefSeq	YP_001963911.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6388751.
GenomeReviews	Gene locus LBF_2858 in contig CP000777_GR.
KEGG	lbf:LBF_2858.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TTDLNFI.
Family and domain databases
HAMAP	MF_01681. [Tree]
InterPro	IPR005834. Dehalogen-like_hydro. IPR010041. Enolase_ppase. [Graphical view]
PANTHER	PTHR20371. Enolase_ppase. 1 hit.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01691. enolase-ppase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNC_LEPBA

Accession

Primary (citable) accession number: B0SFG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	April 8, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)