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B0SBW4

- SYI_LEPBA

UniProt

B0SBW4 - SYI_LEPBA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Leptospira biflexa serovar Patoc (strain Patoc 1 / Ames)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei557 – 5571Aminoacyl-adenylateUniRule annotation
    Binding sitei601 – 6011ATPUniRule annotation
    Metal bindingi887 – 8871ZincUniRule annotation
    Metal bindingi890 – 8901ZincUniRule annotation
    Metal bindingi902 – 9021ZincUniRule annotation
    Metal bindingi905 – 9051ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciLBIF355278:GHTJ-2219-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:LBF_2221
    OrganismiLeptospira biflexa serovar Patoc (strain Patoc 1 / Ames)
    Taxonomic identifieri355278 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
    ProteomesiUP000001846: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 915915Isoleucine--tRNA ligasePRO_1000189177Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi355278.LBF_2221.

    Structurei

    3D structure databases

    ProteinModelPortaliB0SBW4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi64 – 7411"HIGH" regionAdd
    BLAST
    Motifi598 – 6025"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKESHFDE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B0SBW4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKPETENPY SKTVLLPETS FPMKADLAKR EPGQIKVWKD QKVFLNMKEI    50
    RKSKPSFVLH DGPPYANGNF HVGHSLNKIL KDIIIKSKTL SGYQTDMIPG 100
    WDCHGLPIEV QVLKNLGKEA RNTGPSELRK KCREYAAEFV GKQGEDLNRF 150
    LCFWDENNKY LTMAPEFEAR IVEVFGSLFA KGYIYKGKKP VYWCIDLATA 200
    HAEAEIEYQN HVSPSIYVKF AVKGETDTHC LIWTTTPWTL PANLAICFNE 250
    ELPYSLFQSD AHGRLILADG LKEAVEQKTG ITLTKIKSLS NADLKQMVFL 300
    HPFLDRESIP LFGNHVTLDA GTGCVHTAPG HGTDDYRVGT AAGLPTLSPV 350
    DDYGRYTDEF EMMKGIKIWD ANPKIVELLR EKNALVHFSE FTHSYPHSWR 400
    SKKPLIFRAT PQWFFSIDHN GLRDESLKAI DKVQWIPDWG ITRIRSMVES 450
    RPDWCLSRQR NWGVPIPSFT CKSCGLTHLD DKTIQHFIQI VKKEGIEVWY 500
    EKEAKDLLPA DTKCSNCGSE DLKQDKDILD VWFDSGVSSF AVFGDSIGKE 550
    PADLYLEGSD QHRGWFQSSL WPSMAIRKTP PYKSVLTHGY VLDEKGHAMS 600
    KSLGNVINPT TDIINQYGAD ILRLWVSTQD FRDDVKIGKD SIKTVSEAYR 650
    KIRNTFRYLL GNTSAETLTW NLKKEELDTI DKYYLHKLAK LNDEVKKLYD 700
    TYQFHQVYHK ILGFCTVDLS QDYFEIIRDR MYCDAKESKT RRSSEYTLAV 750
    ILEVLSKLLA PILSFTTEEV WTSFGKKDSV FYSDFSDLTE WLDESLESKM 800
    KPVFETKEDV QKALEEARKL GKLGKSLEAE VVIDGKKDSL PFSSEELALF 850
    FVVSHVHFET NGIQEVFSEW KGESGSIQIR KPKHFECPRC WRHVSETEGK 900
    LCKRCDEVVS KLSPN 915
    Length:915
    Mass (Da):104,704
    Last modified:April 8, 2008 - v1
    Checksum:iF31CD5A61F29C54F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000777 Genomic DNA. Translation: ABZ94718.1.
    RefSeqiWP_012389250.1. NC_010842.1.
    YP_001963296.1. NC_010842.1.

    Genome annotation databases

    EnsemblBacteriaiABZ94718; ABZ94718; LBF_2221.
    GeneIDi6388981.
    KEGGilbf:LBF_2221.
    PATRICi22343521. VBILepBif95142_2311.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000777 Genomic DNA. Translation: ABZ94718.1 .
    RefSeqi WP_012389250.1. NC_010842.1.
    YP_001963296.1. NC_010842.1.

    3D structure databases

    ProteinModelPortali B0SBW4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 355278.LBF_2221.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABZ94718 ; ABZ94718 ; LBF_2221 .
    GeneIDi 6388981.
    KEGGi lbf:LBF_2221.
    PATRICi 22343521. VBILepBif95142_2311.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KESHFDE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci LBIF355278:GHTJ-2219-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis."
      Picardeau M., Bulach D.M., Bouchier C., Zuerner R.L., Zidane N., Wilson P.J., Creno S., Kuczek E.S., Bommezzadri S., Davis J.C., McGrath A., Johnson M.J., Boursaux-Eude C., Seemann T., Rouy Z., Coppel R.L., Rood J.I., Lajus A.
      , Davies J.K., Medigue C., Adler B.
      PLoS ONE 3:E1607-E1607(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Patoc 1 / Ames.

    Entry informationi

    Entry nameiSYI_LEPBA
    AccessioniPrimary (citable) accession number: B0SBW4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3