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B0SAB8 (SYL_LEPBA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:LBF_0255
OrganismLeptospira biflexa serovar Patoc (strain Patoc 1 / Ames) [Complete proteome] [HAMAP]
Taxonomic identifier355278 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira

Protein attributes

Sequence length867 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 867867Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091331

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif638 – 6425"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6411ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B0SAB8 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: B2315D585FBDB924

FASTA86799,943
        10         20         30         40         50         60 
MNYPFQDIEQ KWQKYWDHHQ TFRTNTHSSK PKYYCLDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
ISRLKRMEGY EVLHPMGWDA FGLPAERYAM TTGIHPRTTT KNNIDTFRRQ IKSLGLSYDW 

       130        140        150        160        170        180 
EREISTTHPD YYRWTQWIFL QIYNSYFDRK QNKAVPITTL IQTLEREGSL FFEGVELPKG 

       190        200        210        220        230        240 
IQFTASEWKS KSRKEKEDIL SHFRLVYEAN IPVNWCEALG TVLANEEVEE WTSKGYSVER 

       250        260        270        280        290        300 
KPMRQYMMRI TAYAERLLND LSLCEWPPST LEMQRNWIGK SEGLELSFHV PSLNKDITVY 

       310        320        330        340        350        360 
TTRPDTIFGA TYLVLAPEHA LVAELTTPEQ TEAVGQYQKD CSLKSDLERT ELNKDKTGVF 

       370        380        390        400        410        420 
TGAYAQLPTD PSVKVPIYIS DYVLISYGTG AIMAVPAHDQ RDYDFAVKFN LPIKQVIDGK 

       430        440        450        460        470        480 
METNLAFDSK DSVCINSSSA EVQLDGKSYK DAFQTMVVWA EKKGIGRKKI QFKLRDWLFA 

       490        500        510        520        530        540 
RQRYWGEPIP LVHFEDGSPK ALSDSELPLV LPDLEEFKPS GTGESPLALA KDWLVYKDPE 

       550        560        570        580        590        600 
TGEIGKRETN TMPQWAGSCY YYLRYIDPRN NDKLIDPELE KMWMPVEVYV GGAEHAVLHL 

       610        620        630        640        650        660 
LYSRFWHKIL FDLGHVSTPE PFKKLVHQGL ILGEDKGKMS KSRGNVVNPD DVVSEFGADT 

       670        680        690        700        710        720 
LRLFEMFMGP FEMSKPWSKN GVDGVFRFLN RVWRLFHSGE NESFFVEDIE PNEAEQKTLH 

       730        740        750        760        770        780 
RTIKKVKDDI DSFSFNTAVS QMMIFINEFT SNPRKPKQVL EPFVLALSPF APHLAEELWA 

       790        800        810        820        830        840 
KLGHTDSLAY HPYPKWDEKY LIDANITIVV QVNGKMRGEF LAPREIEEKE ALSLAKEVEK 

       850        860 
AKPFWVGKEI KKEIYVKGKL VNIVVAG 

« Hide

References

[1]"Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis."
Picardeau M., Bulach D.M., Bouchier C., Zuerner R.L., Zidane N., Wilson P.J., Creno S., Kuczek E.S., Bommezzadri S., Davis J.C., McGrath A., Johnson M.J., Boursaux-Eude C., Seemann T., Rouy Z., Coppel R.L., Rood J.I., Lajus A. expand/collapse author list , Davies J.K., Medigue C., Adler B.
PLoS ONE 3:E1607-E1607(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Patoc 1 / Ames.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000777 Genomic DNA. Translation: ABZ92800.1.
RefSeqYP_001961378.1. NC_010842.1.

3D structure databases

ProteinModelPortalB0SAB8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING355278.LBF_0255.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ92800; ABZ92800; LBF_0255.
GeneID6387435.
KEGGlbf:LBF_0255.
PATRIC22339379. VBILepBif95142_0268.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycLBIF355278:GHTJ-255-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LEPBA
AccessionPrimary (citable) accession number: B0SAB8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 8, 2008
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries