B0S8T0 (B0S8T0_LEPBA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 2 HAMAP MF_00444
EC=3.4.21.92 HAMAP MF_00444

Alternative name(s):
Endopeptidase Clp 2 HAMAP MF_00444

Gene names

Name:	clpP-2 EMBL ABZ94161.1
Synonyms:	clpP2 HAMAP MF_00444
Ordered Locus Names:	LBF_1654

Organism

Leptospira biflexa serovar Patoc (strain Patoc 1 / Ames) [Complete proteome] [HAMAP]

Taxonomic identifier

355278 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444 RuleBase RU000550
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444 RuleBase RU000549
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family. HAMAP MF_00444 RuleBase RU003567

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_00444
Ligand	ATP-binding HAMAP MF_00444 Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease HAMAP MF_00444 RuleBase RU000549
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	102	1	By similarity HAMAP MF_00444
	Active site	127	1	By similarity HAMAP MF_00444

Sequences

	Sequence		Length	Mass (Da)
	B0S8T0 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 6CF3EC9BEE3ED355	FASTA	202	22,421
	10 20 30 40 50 60 MPEEETPEKE ITETIQDLIS DKNMGKKFLE KRKIFLWGPV TDESSKELTA KLMYLEMVDP 70 80 90 100 110 120 GKPITFYINS PGGVVTSGLV VYDTMQMISS PVHTVCMGMA ASMGSILLIG GKKGNRYIWP 130 140 150 160 170 180 NGRVMIHQPS IGGQIQAPAT DLLIHAQDIV KTKEKLNQML ADACGKSYEQ LVEDTDRDYY 190 200 MDAEQALAYG IVDKIVNTID VV

References

[1]

"Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis." Picardeau M., Bulach D.M., Bouchier C., Zuerner R.L., Zidane N., Wilson P.J., Creno S., Kuczek E.S., Bommezzadri S., Davis J.C., McGrath A., Johnson M.J., Boursaux-Eude C., Seemann T., Rouy Z., Coppel R.L., Rood J.I., Lajus A. , Davies J.K., Medigue C., Adler B. PLoS ONE 3:E1607-E1607(2008) [PubMed: 18270594] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000777 Genomic DNA. Translation: ABZ94161.1.
RefSeq	YP_001962739.1. NC_010842.1.
3D structure databases
ProteinModelPortal	B0S8T0.
ModBase	Search...
Protein-protein interaction databases
STRING	B0S8T0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6387602.
GenomeReviews	Gene locus LBF_1654 in contig CP000777_GR.
KEGG	lbf:LBF_1654.
PATRIC	22342401. VBILepBif95142_1756.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG558421.
OMA	FSEPVTD.
ProtClustDB	CLSK574113.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B0S8T0_LEPBA

Accession

Primary (citable) accession number: B0S8T0

Entry history

Integrated into UniProtKB/TrEMBL:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)