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Protein

Solute carrier family 15 member 2

Gene

slc15a2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Proton-coupled intake of oligopeptides of 2 to 4 amino acids with a preference for dipeptides.1 Publication

Kineticsi

  1. KM=97 µM for Gly-L-Gln (at pH 5.5 and -160 mV)1 Publication
  2. KM=79 µM for Gly-L-Gln (at pH 6.5 and -160 mV)1 Publication
  3. KM=48 µM for Gly-L-Gln (at pH 7.5 and -160 mV)1 Publication
  4. KM=163 µM for Gly-L-Gln (at pH 8.5 and -160 mV)1 Publication
  5. KM=82 µM for Gly-L-Gln (at pH 5.5 and -120 mV)1 Publication
  6. KM=38 µM for Gly-L-Gln (at pH 6.5 and -120 mV)1 Publication
  7. KM=18 µM for Gly-L-Gln (at pH 7.5 and -120 mV)1 Publication
  8. KM=118 µM for Gly-L-Gln (at pH 8.5 and -120 mV)1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    GO - Molecular functioni

    • dipeptide transmembrane transporter activity Source: ZFIN

    GO - Biological processi

    • response to yeast Source: ZFIN

    Keywordsi

    Biological processPeptide transport, Protein transport, Symport, Transport

    Enzyme and pathway databases

    ReactomeiR-DRE-427975 Proton/oligopeptide cotransporters

    Protein family/group databases

    TCDBi2.A.17.4.5 the proton-dependent oligopeptide transporter (pot/ptr) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Solute carrier family 15 member 21 Publication
    Alternative name(s):
    Peptide transporter 21 Publication
    Short name:
    PEPT21 Publication
    Gene namesi
    Name:slc15a21 PublicationImported
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    Proteomesi
    • UP000000437 Componenti: Chromosome 9

    Organism-specific databases

    ZFINiZDB-GENE-030131-5866 slc15a2

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 43CytoplasmicCuratedAdd BLAST43
    Transmembranei44 – 61HelicalSequence analysisAdd BLAST18
    Topological domaini62 – 69ExtracellularCurated8
    Transmembranei70 – 90HelicalSequence analysisAdd BLAST21
    Topological domaini91 – 99CytoplasmicCurated9
    Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
    Topological domaini121 – 125ExtracellularCurated5
    Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
    Topological domaini147 – 169CytoplasmicCuratedAdd BLAST23
    Transmembranei170 – 190HelicalSequence analysisAdd BLAST21
    Topological domaini191 – 201ExtracellularCuratedAdd BLAST11
    Transmembranei202 – 222HelicalSequence analysisAdd BLAST21
    Topological domaini223 – 280CytoplasmicCuratedAdd BLAST58
    Transmembranei281 – 301HelicalSequence analysisAdd BLAST21
    Topological domaini302 – 334ExtracellularCuratedAdd BLAST33
    Transmembranei335 – 355HelicalSequence analysisAdd BLAST21
    Topological domaini356 – 367CytoplasmicCuratedAdd BLAST12
    Transmembranei368 – 388HelicalSequence analysisAdd BLAST21
    Topological domaini389 – 594ExtracellularCuratedAdd BLAST206
    Transmembranei595 – 615HelicalSequence analysisAdd BLAST21
    Topological domaini616 – 626CytoplasmicCuratedAdd BLAST11
    Transmembranei627 – 647HelicalSequence analysisAdd BLAST21
    Topological domaini648 – 657ExtracellularCurated10
    Transmembranei658 – 678HelicalSequence analysisAdd BLAST21
    Topological domaini679 – 719CytoplasmicCuratedAdd BLAST41

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004443191 – 719Solute carrier family 15 member 2Add BLAST719

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi66N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
    Glycosylationi481N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
    Glycosylationi513N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
    Glycosylationi532N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiB0S6T2

    Expressioni

    Tissue specificityi

    Expressed in kidney, brain and gut. Also expressed weakly in eye, gill and skeletal muscle.1 Publication

    Developmental stagei

    Detected at 1 to 7 days post-fertilization (dpf). Expressed in the forebrain and midbrain at 1 dpf, where it localizes to the ependymal cell layer of the dicephalic ventricle and midbrain ventricle. Expressed in the developing otic vesicle at 2-7 dpf, where it shows particularly strong expression in the epithelium of the semicircular canal projections.1 Publication

    Gene expression databases

    BgeeiENSDARG00000032010
    ExpressionAtlasiB0S6T2 baseline

    Interactioni

    Protein-protein interaction databases

    STRINGi7955.ENSDARP00000091395

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237 Eukaryota
    COG3104 LUCA
    GeneTreeiENSGT00530000062961
    HOGENOMiHOG000285186
    HOVERGENiHBG052962
    InParanoidiB0S6T2
    KOiK14637
    OMAiFHLKYHN
    OrthoDBiEOG091G0M1S
    TreeFamiTF330897

    Family and domain databases

    CDDicd06174 MFS, 1 hit
    InterProiView protein in InterPro
    IPR020846 MFS_dom
    IPR036259 MFS_trans_sf
    IPR000109 POT_fam
    IPR018456 PTR2_symporter_CS
    PANTHERiPTHR11654 PTHR11654, 1 hit
    PfamiView protein in Pfam
    PF00854 PTR2, 2 hits
    SUPFAMiSSF103473 SSF103473, 2 hits
    PROSITEiView protein in PROSITE
    PS01022 PTR2_1, 1 hit

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: B0S6T2-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MGKMKDKDVD AEKYEKAQRS PKLCGTNYPV SIAFIVVNEF CERFSYYGMK
    60 70 80 90 100
    AVLTLYFMNY LHWDKNLSTA IYHAFSGLCY FTPLLGALIA DSWLGKFKTI
    110 120 130 140 150
    IYLSIVYVIG HVVKSVGAIP DVGDSTVHIA LSMVGLGLIA LGTGGIKPCV
    160 170 180 190 200
    AAFGGDQFDE DNIDERRKFF SIFYMSINAG SVLSTIITPI LRGDVQCFGG
    210 220 230 240 250
    DCYALAFGVP AALMVIALVV FISGSGLYKK SPPEGNVLVR VCKCIGFAIS
    260 270 280 290 300
    NRWTNSKKSP KRSHWLDWAE EKYSKRLIQE IKMVCRVLVL YIPLPMFWAL
    310 320 330 340 350
    FDQQGSRWTL QATRMNMDFG GGFIIKPDQM QMLNALLILV FIPIFDMGIY
    360 370 380 390 400
    PLVGLCRIKL TPLKKMATGM ILAALAFCAA TAVEVYVIKT VVEPPPAKES
    410 420 430 440 450
    LVQVYNLMDS DVTVQFPAHN VFSEPLKPYE EPSGYSSLPL TGESQLQNVV
    460 470 480 490 500
    VSHNGMNYQC RLTFTERMAY SLLLHPTAQH NGSVCNLVKD HITKSETGAA
    510 520 530 540 550
    YIRFINTHTE NINVTVGTEE VHASANYGIS RNISVPRGEY NKAVCVTDTK
    560 570 580 590 600
    EYEINLGLLD FGAFYTVILS EAGNNLAVKK MEDIQANNIH IGWQIPQYVF
    610 620 630 640 650
    LTAGEVMFSI TGLEFSYSQA PASMKSVLQA GWLMTVAFGN VIVLIVAEGA
    660 670 680 690 700
    GMEQWVEFLL FAALLVAVSI IFSIMAYFYT YVDPDQLDKL FKEDGDGGKV
    710
    ESSKKDELSL GDMPKQTKM
    Length:719
    Mass (Da):79,690
    Last modified:April 8, 2008 - v1
    Checksum:i7026718548852604
    GO
    Isoform 2 (identifier: B0S6T2-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         280-304: Missing.

    Show »
    Length:694
    Mass (Da):76,654
    Checksum:i1F7766B1EE143890
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti456 – 459MNYQ → VNYE in ABA86557 (PubMed:16317081).Curated4
    Sequence conflicti456 – 459MNYQ → VNYE in AAI24582 (Ref. 3) Curated4
    Sequence conflicti456 – 459MNYQ → VNYE in AAI65646 (Ref. 3) Curated4
    Sequence conflicti477 – 481TAQHN → PAQSG in ABA86557 (PubMed:16317081).Curated5
    Sequence conflicti477 – 481TAQHN → PAQSG in AAI24582 (Ref. 3) Curated5
    Sequence conflicti477 – 481TAQHN → PAQSG in AAI65646 (Ref. 3) Curated5
    Sequence conflicti549T → S in ABA86557 (PubMed:16317081).Curated1
    Sequence conflicti549T → S in AAI24582 (Ref. 3) Curated1
    Sequence conflicti549T → S in AAI65646 (Ref. 3) Curated1
    Sequence conflicti572A → T in ABA86557 (PubMed:16317081).Curated1
    Sequence conflicti572A → T in AAI24582 (Ref. 3) Curated1
    Sequence conflicti572A → T in AAI65646 (Ref. 3) Curated1
    Sequence conflicti578V → I in ABA86557 (PubMed:16317081).Curated1
    Sequence conflicti578V → I in AAI24582 (Ref. 3) Curated1
    Sequence conflicti578V → I in AAI65646 (Ref. 3) Curated1
    Sequence conflicti694D → E in ABA86557 (PubMed:16317081).Curated1
    Sequence conflicti694D → E in AAI24582 (Ref. 3) Curated1
    Sequence conflicti694D → E in AAI65646 (Ref. 3) Curated1
    Sequence conflicti712 – 713DM → EI in ABA86557 (PubMed:16317081).Curated2
    Sequence conflicti712 – 713DM → EI in AAI24582 (Ref. 3) Curated2
    Sequence conflicti712 – 713DM → EI in AAI65646 (Ref. 3) Curated2

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_059583280 – 304Missing in isoform 2. Add BLAST25

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ192597 mRNA Translation: ABA86557.1
    BX548071 Genomic DNA No translation available.
    BC124581 mRNA Translation: AAI24582.1
    BC165646 mRNA Translation: AAI65646.1
    RefSeqiNP_001034917.1, NM_001039828.1
    UniGeneiDr.79496

    Genome annotation databases

    EnsembliENSDART00000100622; ENSDARP00000091395; ENSDARG00000032010
    GeneIDi556684
    KEGGidre:556684

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiS15A2_DANRE
    AccessioniPrimary (citable) accession number: B0S6T2
    Secondary accession number(s): Q08BT0, Q2F800
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 23, 2018
    Last sequence update: April 8, 2008
    Last modified: May 23, 2018
    This is version 67 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

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