Reviewed, UniProtKB/Swiss-Prot B0S1S2 (NADE_FINM2)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	FMG_0894

Organism

Finegoldia magna (strain ATCC 29328) (Peptostreptococcus magnus) [Complete proteome] [HAMAP]

Taxonomic identifier

334413 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales Family XI. Incertae Sedis › Finegoldia

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099022
Regions
	Nucleotide binding	29 – 36	8	ATP By similarity
Sites
	Active site	31	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0S1S2-1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 8BD073647667F70D	FASTA	241	27,246
	10 20 30 40 50 60 MNYAKLCEDL TKWIKEEVES ANLKGAVFGI SGGIDSAVLA CLCKKAFGDN ALGLIMPIKS 70 80 90 100 110 120 NPKDEEDARI LAKSIGLRFT KVDLNESYDA LIGTFEKNSV EMAASNIKPR LRMITLYYYA 130 140 150 160 170 180 QNNGYMVLSG SNRSEFMTGY FTKYGDSGAD LMPLLNLYKT DIFEMAKVLG VPDVIINKKP 190 200 210 220 230 240 SAGLWEGQTD EDEFGFTYEE LDDYLMNNSN TKSKDLIDKK IKQSEHKRKF AKSFEFDRRN Y

References

[1]

"Complete genome sequence of Finegoldia magna, an anaerobic opportunistic pathogen." Goto T., Yamashita A., Hirakawa H., Matsutani M., Todo K., Ohshima K., Toh H., Miyamoto K., Kuhara S., Hattori M., Shimizu T., Akimoto S. DNA Res. 15:39-47(2008) [PubMed: 18263572] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008971 Genomic DNA. Translation: BAG08312.1.
RefSeq	YP_001692202.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6018731.
GenomeReviews	Gene locus FMG_0894 in contig AP008971_GR.
KEGG	fma:FMG_0894.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CAINPIG.
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_FINM2

Accession

Primary (citable) accession number: B0S1S2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)