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B0S101 (SYL_FINM2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:FMG_0623
OrganismFinegoldia magna (strain ATCC 29328) (Peptostreptococcus magnus) [Complete proteome] [HAMAP]
Taxonomic identifier334413 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiales Family XI. Incertae SedisFinegoldia

Protein attributes

Sequence length802 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 802802Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091319

Regions

Motif41 – 5212"HIGH" region HAMAP-Rule MF_00049
Motif581 – 5855"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5841ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B0S101 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 06571C3611F17057

FASTA80293,768
        10         20         30         40         50         60 
MDKYNPNSIE KKWQKYWEEN KTFKTSDDKS KKRFYALVEF PYPSGQGLHV GHPRPYTALD 

        70         80         90        100        110        120 
IVSRKRRMQG YNVLYPMGWD AFGLPTENFA IKNKIRPEVV TENNIKNFKR QMQSIGFSFD 

       130        140        150        160        170        180 
WDREINTTDP DYYKWTQWIF IQMFKKGLAY KKEMPINWCP SCKTGLANEE VINGHCERCG 

       190        200        210        220        230        240 
GQVVRKVKNQ WMLKITEYAD RLIDDLKDVD YFDRIKSQQI NWIGRSYGAE INFAVKEVDE 

       250        260        270        280        290        300 
KITVFTTRAD TIFGATYMVI SVDHPLIEKY SDRIKNIDEI RSYRSEVAKK SELERTDLSK 

       310        320        330        340        350        360 
EKTGYKIDGL TAINPLTNKE IPVYVSDYVL MTYGTGAIMA VPAHDDRDYE FAKKFNIEMI 

       370        380        390        400        410        420 
PVIEGSDIQN CAFTETNEGN LINSGFLNGL TVDEAKEKMY EYIEEKEIGH KKTNYKLRDW 

       430        440        450        460        470        480 
VFSRQRYWGE PIPLVYCEHC GWVPLDEKDL PLVLPKVDNY EPTDNGESPL SKIDDFVHTK 

       490        500        510        520        530        540 
CPKCGRDAVR ETDTMPQWAG SSWYYLRYTD PHNDEAIASK ENLDYYTPVD WYNGGMEHTT 

       550        560        570        580        590        600 
LHLLYSRFWH KFLYDIGVVP TKEPYMKRTS HGMILGDNNE KMSKSRGNVV NPDDIVRDFG 

       610        620        630        640        650        660 
ADTLRCYEMF IGDFEKSAPW SENGVKGCRK FLDKVWRTQD LVDGDSNFEK METLIHQTIK 

       670        680        690        700        710        720 
KVSEDYENLK FNTAIAQLMT LLNEFNNLDK ISKEQFKIFL ILLNPVCPHI TEEIWQRMGY 

       730        740        750        760        770        780 
EGYVHESSWP EYDESKTILD VIELPIQVNG KLRATVEINR EASEDEVYEK AVKDDVVAKY 

       790        800 
LENKNVVKKI YVKGRIFNII VK 

« Hide

References

[1]"Complete genome sequence of Finegoldia magna, an anaerobic opportunistic pathogen."
Goto T., Yamashita A., Hirakawa H., Matsutani M., Todo K., Ohshima K., Toh H., Miyamoto K., Kuhara S., Hattori M., Shimizu T., Akimoto S.
DNA Res. 15:39-47(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29328.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008971 Genomic DNA. Translation: BAG08041.1.
RefSeqYP_001691931.1. NC_010376.1.

3D structure databases

ProteinModelPortalB0S101.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING334413.FMG_0623.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAG08041; BAG08041; FMG_0623.
GeneID6020246.
KEGGfma:FMG_0623.
PATRIC21886909. VBIFinMag33027_0828.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMANENGVEG.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycFMAG334413:GJ6M-667-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_FINM2
AccessionPrimary (citable) accession number: B0S101
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries