B0S0W2 (SYE_FINM2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	FMG_0414

Organism

Finegoldia magna (strain ATCC 29328) (Peptostreptococcus magnus) [Complete proteome] [HAMAP]

Taxonomic identifier

334413 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales Family XI. Incertae Sedis › Finegoldia

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 489	489	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000367674
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	261 – 265	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	264	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0S0W2 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 9FA480FE08A462D0	FASTA	489	56,324
	10 20 30 40 50 60 MSEVRVRFAP SPTGFLHIGG LRTALYNYLY AKRNNGKFLL RIEDTDRTRY VEGAIENLLE 70 80 90 100 110 120 QLKWAGLDPD EGVVLDDEGN VTEVGECGPY IQSDRVKQGL YQKYIDELIE KGYAYYCFCS 130 140 150 160 170 180 KERLDQVKAQ QKADGLMPKY DGLCRGISIE DAKKRIANGE EYVIRLKLPE NKEITFNDAI 190 200 210 220 230 240 KGKITFNTND MDDQVLIKSD GFPTYHFAVV VDDHLMGITH IVRGDEWISS TAKHVYLYQC 250 260 270 280 290 300 FGWDVPEFVH LPVVLNKSGK KLSKRNDDVA VKDFRKKGYL PEAIDNYLAL VGWSSEDNQE 310 320 330 340 350 360 IMSMEELKHK FDFNRVSKSG GVFDTEKLNW INRHYIKEIE NEKLASMLKP YLVEDGVISE 370 380 390 400 410 420 DYPEYKLIEI ASLFKEELDY MAEITEKVEF LFKDYEMDDD AKEFLNYEKL DELMNALKEE 430 440 450 460 470 480 IESVDEIEKE FASGVMKKVQ KKTGIKGKDL WMTTRAVVTG NVHGPDLDSI MVVLGKQEVL DRINKALNR

References

[1]

"Complete genome sequence of Finegoldia magna, an anaerobic opportunistic pathogen." Goto T., Yamashita A., Hirakawa H., Matsutani M., Todo K., Ohshima K., Toh H., Miyamoto K., Kuhara S., Hattori M., Shimizu T., Akimoto S. DNA Res. 15:39-47(2008) [PubMed: 18263572] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29328.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008971 Genomic DNA. Translation: BAG07832.1.
RefSeq	YP_001691722.1. NC_010376.1.
3D structure databases
ProteinModelPortal	B0S0W2.
ModBase	Search...
Protein-protein interaction databases
STRING	B0S0W2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6019878.
GenomeReviews	Gene locus FMG_0414 in contig AP008971_GR.
KEGG	fma:FMG_0414.
PATRIC	21886439. VBIFinMag33027_0619.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	DKETAND.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	FMAG334413:FMG_0414-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K09698.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_FINM2

Accession

Primary (citable) accession number: B0S0W2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program