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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Finegoldia magna (strain ATCC 29328) (Peptostreptococcus magnus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei264 – 2641ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciFMAG334413:GJ6M-432-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:FMG_0414
OrganismiFinegoldia magna (strain ATCC 29328) (Peptostreptococcus magnus)
Taxonomic identifieri334413 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptoniphilaceaeFinegoldia
ProteomesiUP000001319 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Glutamate--tRNA ligasePRO_0000367674Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi334413.FMG_0414.

Structurei

3D structure databases

ProteinModelPortaliB0S0W2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 2011"HIGH" regionAdd
BLAST
Motifi261 – 2655"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK09698.
OMAiKLNWINS.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0S0W2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEVRVRFAP SPTGFLHIGG LRTALYNYLY AKRNNGKFLL RIEDTDRTRY
60 70 80 90 100
VEGAIENLLE QLKWAGLDPD EGVVLDDEGN VTEVGECGPY IQSDRVKQGL
110 120 130 140 150
YQKYIDELIE KGYAYYCFCS KERLDQVKAQ QKADGLMPKY DGLCRGISIE
160 170 180 190 200
DAKKRIANGE EYVIRLKLPE NKEITFNDAI KGKITFNTND MDDQVLIKSD
210 220 230 240 250
GFPTYHFAVV VDDHLMGITH IVRGDEWISS TAKHVYLYQC FGWDVPEFVH
260 270 280 290 300
LPVVLNKSGK KLSKRNDDVA VKDFRKKGYL PEAIDNYLAL VGWSSEDNQE
310 320 330 340 350
IMSMEELKHK FDFNRVSKSG GVFDTEKLNW INRHYIKEIE NEKLASMLKP
360 370 380 390 400
YLVEDGVISE DYPEYKLIEI ASLFKEELDY MAEITEKVEF LFKDYEMDDD
410 420 430 440 450
AKEFLNYEKL DELMNALKEE IESVDEIEKE FASGVMKKVQ KKTGIKGKDL
460 470 480
WMTTRAVVTG NVHGPDLDSI MVVLGKQEVL DRINKALNR
Length:489
Mass (Da):56,324
Last modified:April 8, 2008 - v1
Checksum:i9FA480FE08A462D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008971 Genomic DNA. Translation: BAG07832.1.
RefSeqiYP_001691722.1. NC_010376.1.

Genome annotation databases

EnsemblBacteriaiBAG07832; BAG07832; FMG_0414.
KEGGifma:FMG_0414.
PATRICi21886439. VBIFinMag33027_0619.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008971 Genomic DNA. Translation: BAG07832.1.
RefSeqiYP_001691722.1. NC_010376.1.

3D structure databases

ProteinModelPortaliB0S0W2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi334413.FMG_0414.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAG07832; BAG07832; FMG_0414.
KEGGifma:FMG_0414.
PATRICi21886439. VBIFinMag33027_0619.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK09698.
OMAiKLNWINS.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciFMAG334413:GJ6M-432-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Finegoldia magna, an anaerobic opportunistic pathogen."
    Goto T., Yamashita A., Hirakawa H., Matsutani M., Todo K., Ohshima K., Toh H., Miyamoto K., Kuhara S., Hattori M., Shimizu T., Akimoto S.
    DNA Res. 15:39-47(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29328.

Entry informationi

Entry nameiSYE_FINM2
AccessioniPrimary (citable) accession number: B0S0W2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 8, 2008
Last modified: April 1, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.