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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Xanthomonas campestris pv. campestris (strain B100)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei577 – 5771Aminoacyl-adenylateUniRule annotation
Binding sitei621 – 6211ATPUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation
Metal bindingi929 – 9291ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciXCAM509169:GHW4-3250-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:xcc-b100_3183
OrganismiXanthomonas campestris pv. campestris (strain B100)
Taxonomic identifieri509169 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
ProteomesiUP000001188 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Isoleucine--tRNA ligasePRO_1000189216Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB0RY28.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi618 – 6225"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0RY28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQDYKATLH LPATDFPMRG DLPKREPAML ERWEREGLYA QVRANAAGRP
60 70 80 90 100
LFVLHDGPPY ANGQIHLGHA VNKILKDIIV KSKYLAGFDA PYIPGWDCHG
110 120 130 140 150
LPIEIAIEKK FGKVGVKLDA AQFRQKCREY ATEQIDLQRR DFKRLGVIGD
160 170 180 190 200
WDNPYKTLDF RFEANEIRAL AKVIDNGHLT RGVKPVHWCF DCGSALAEAE
210 220 230 240 250
IEYADKVSPT VDIAYPARDP AAVAAAFGVT LSAGTQVAVP IWTTTPWTLP
260 270 280 290 300
ASLAVSLGAE LDYVLVEGAA DHGQPRWLVI AEALAGKALA RYGVDAVVVH
310 320 330 340 350
GHAKGAALDQ LLLAHPFYAE RDIPLILGDH VSDDDGTGAV HTAPGHGQED
360 370 380 390 400
YQVSKQYGLL ERYSAAQINP IDGRGVYLPS TPPLGDTVLA GLHIWKANDV
410 420 430 440 450
IIDALRDTGA LLAASKMEHS YPHCWRHKTP IAFRATPQWF ISMEQANLRA
460 470 480 490 500
DALKAIETVH WYPSWGQARI AGMIDGRPDW TISRQRTWGV PIALFVHRET
510 520 530 540 550
GEPHPRSTEL MRQVAERVEQ GGVDVWYTLD AAELLGDEAG DYDKITDILD
560 570 580 590 600
VWFDSGVTHE TVLVDRGLPK PADLYLEGSD QHRGWFQSSL LTGVAMDKVA
610 620 630 640 650
PYKQCLTHGF TVDEHGRKMS KSLGNGIEPQ EIMRTLGADI LRLWIASADY
660 670 680 690 700
SNEMSLSQEI LKRNADAYRR LRNTARFLLG NLHGFDPLQH LVALDDMVLL
710 720 730 740 750
DRWIVHRAHE LQEKITAAYA RYDFAEIVQG LLNFCSVDLG SLYLDVTKDR
760 770 780 790 800
LYTMAEDARG RRSAQSAMYH VAEAFVRWIA PVLSFTAEEL WAYLPGEHSG
810 820 830 840 850
NVLFATWYDG LAPMPADAAL TSADVDKLLA LREQVAKVLE PMRANGAIGA
860 870 880 890 900
ALEAEITVAA DAQTAARWQP LSDELRFLFI SGDVTVTAAS TDDIFVSAQP
910 920 930 940
TTKAKCVRCW HHQASVGSDP RHPELCSRCV SNIEGPGEER RWF
Length:943
Mass (Da):104,524
Last modified:April 8, 2008 - v1
Checksum:i5C5D14E3FFEA0CB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM920689 Genomic DNA. Translation: CAP52548.1.

Genome annotation databases

EnsemblBacteriaiCAP52548; CAP52548; xcc-b100_3183.
KEGGixca:xccb100_3183.
PATRICi24086268. VBIXanCam108527_3211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM920689 Genomic DNA. Translation: CAP52548.1.

3D structure databases

ProteinModelPortaliB0RY28.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAP52548; CAP52548; xcc-b100_3183.
KEGGixca:xccb100_3183.
PATRICi24086268. VBIXanCam108527_3211.

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciXCAM509169:GHW4-3250-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis."
    Vorhoelter F.-J., Schneiker S., Goesmann A., Krause L., Bekel T., Kaiser O., Linke B., Patschkowski T., Rueckert C., Schmid J., Sidhu V.K., Sieber V., Tauch A., Watt S.A., Weisshaar B., Becker A., Niehaus K., Puehler A.
    J. Biotechnol. 134:33-45(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B100.

Entry informationi

Entry nameiSYI_XANCB
AccessioniPrimary (citable) accession number: B0RY28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 8, 2008
Last modified: July 22, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.