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B0RV20 (B0RV20_XANCB) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Endonuclease III PIRNR PIRNR001435 HAMAP-Rule MF_00942

EC=4.2.99.18 PIRNR PIRNR001435 HAMAP-Rule MF_00942
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase HAMAP-Rule MF_00942
Gene names
Name:nth HAMAP-Rule MF_00942
Ordered Locus Names:xcc-b100_2728 EMBL CAP52089.1
OrganismXanthomonas campestris pv. campestris (strain B100) [Complete proteome] [HAMAP]
Taxonomic identifier509169 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity By similarity. PIRNR PIRNR001435

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate By similarity. HAMAP-Rule MF_00942

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. PIRNR PIRNR001435 HAMAP-Rule MF_00942

Cofactor

Binds 1 4Fe-4S cluster By similarity. PIRNR PIRNR001435 HAMAP-Rule MF_00942

Sequence similarities

Belongs to the Nth/MutY family. PIRNR PIRNR001435 HAMAP-Rule MF_00942

Contains 1 HhH domain. HAMAP-Rule MF_00942

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain114 – 14229HhH By similarity HAMAP-Rule MF_00942

Sites

Metal binding2021Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_00942
Metal binding2091Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_00942
Metal binding2121Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_00942
Metal binding2181Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_00942

Sequences

Sequence LengthMass (Da)Tools
B0RV20 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 59969F037273436F

FASTA22725,144
        10         20         30         40         50         60 
MSSALSAPPP RRGSTLRKPE IQELFARLRE LNPHPTTELE YTTPFELLIA VLLSAQATDV 

        70         80         90        100        110        120 
GVNKATRKLY PVANTPRDIL DLGEEGLKRY ISTIGLFNAK AKNVIATCRI LLERYGGEVP 

       130        140        150        160        170        180 
HDRAALEALP GVGRKTANVV LNTAFGEPTM AVDTHIFRVA NRTGLAPGKD VRVVEDKLVK 

       190        200        210        220 
VIPAEFLHDA HHWLILHGRY VCKARKPDCP NCVIHDLCRY RDKTVAA 

« Hide

References

[1]"The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis."
Vorhoelter F.-J., Schneiker S., Goesmann A., Krause L., Bekel T., Kaiser O., Linke B., Patschkowski T., Rueckert C., Schmid J., Sidhu V.K., Sieber V., Tauch A., Watt S.A., Weisshaar B., Becker A., Niehaus K., Puehler A.
J. Biotechnol. 134:33-45(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B100 EMBL CAP52089.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM920689 Genomic DNA. Translation: CAP52089.1.
RefSeqYP_001904133.1. NC_010688.1.

3D structure databases

ProteinModelPortalB0RV20.
SMRB0RV20. Positions 16-223.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING509169.xccb100_2728.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAP52089; CAP52089; xcc-b100_2728.
GeneID6322757.
KEGGxca:xccb100_2728.
PATRIC24085375. VBIXanCam108527_2768.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0177.
KOK10773.
OMANNKSKHL.
OrthoDBEOG6H4KC5.
ProtClustDBCLSK446054.

Enzyme and pathway databases

BioCycXCAM509169:GHW4-2788-MONOMER.

Family and domain databases

Gene3D1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPMF_00942. Nth.
InterProIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamPF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
PIRSFPIRSF001435. Nth. 1 hit.
SMARTSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMSSF48150. SSF48150. 1 hit.
TIGRFAMsTIGR01083. nth. 1 hit.
PROSITEPS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB0RV20_XANCB
AccessionPrimary (citable) accession number: B0RV20
Entry history
Integrated into UniProtKB/TrEMBL: May 20, 2008
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)