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Protein

3-hydroxyanthranilate 3,4-dioxygenase

Gene

nbaC

Organism
Xanthomonas campestris pv. campestris (strain B100)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.UniRule annotation

Catalytic activityi

3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 2 Fe2+ ions per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441DioxygenUniRule annotation
Metal bindingi48 – 481Iron 1; catalyticUniRule annotation
Metal bindingi54 – 541Iron 1; catalyticUniRule annotation
Binding sitei54 – 541SubstrateUniRule annotation
Metal bindingi92 – 921Iron 1; catalyticUniRule annotation
Binding sitei96 – 961SubstrateUniRule annotation
Binding sitei106 – 1061SubstrateUniRule annotation
Metal bindingi121 – 1211Iron 2UniRule annotation
Metal bindingi124 – 1241Iron 2UniRule annotation
Metal bindingi158 – 1581Iron 2UniRule annotation
Metal bindingi161 – 1611Iron 2UniRule annotation

GO - Molecular functioni

  1. 3-hydroxyanthranilate 3,4-dioxygenase activity Source: UniProtKB-HAMAP
  2. ferrous iron binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' NAD biosynthetic process from tryptophan Source: UniProtKB-HAMAP
  2. anthranilate metabolic process Source: UniProtKB-HAMAP
  3. quinolinate biosynthetic process Source: UniProtKB-HAMAP
  4. tryptophan catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciXCAM509169:GHW4-2765-MONOMER.
UniPathwayiUPA00253; UER00330.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyanthranilate 3,4-dioxygenaseUniRule annotation (EC:1.13.11.6UniRule annotation)
Alternative name(s):
3-hydroxyanthranilate oxygenaseUniRule annotation
Short name:
3-HAOUniRule annotation
3-hydroxyanthranilic acid dioxygenaseUniRule annotation
Short name:
HADUniRule annotation
Gene namesi
Name:nbaCUniRule annotation
Ordered Locus Names:xcc-b100_2705
OrganismiXanthomonas campestris pv. campestris (strain B100)
Taxonomic identifieri509169 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
ProteomesiUP000001188: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1761763-hydroxyanthranilate 3,4-dioxygenasePRO_1000134551Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi509169.xccb100_2705.

Structurei

3D structure databases

ProteinModelPortaliB0RUZ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-HAO family.UniRule annotation

Phylogenomic databases

eggNOGiNOG77058.
KOiK00452.
OMAiKPPVGNQ.
OrthoDBiEOG6PW234.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_00825. 3_HAO.
InterProiIPR010329. 3hydroanth_dOase.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.

Sequencei

Sequence statusi: Complete.

B0RUZ7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLVPPINLHA WVEQHRHLLK PPVGNKCIQQ DGFIIMIVGG PNARTDYHYD
60 70 80 90 100
EGPEWFFQLE GEMVLKVQDD GTARDIPIRA GEIFLLPPKV PHSPQRAAGS
110 120 130 140 150
IGLVIERERL PHEQDGLQWY CPQCNHKLYE AMFPLENIET DFPPVFDHFY
160 170
RSLALRTCMQ CGHVHPAPER YAAIEA
Length:176
Mass (Da):20,172
Last modified:April 8, 2008 - v1
Checksum:i3C3D78418C8458D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM920689 Genomic DNA. Translation: CAP52066.1.
RefSeqiYP_001904110.1. NC_010688.1.

Genome annotation databases

EnsemblBacteriaiCAP52066; CAP52066; xcc-b100_2705.
GeneIDi6322736.
KEGGixca:xccb100_2705.
PATRICi24085325. VBIXanCam108527_2743.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM920689 Genomic DNA. Translation: CAP52066.1.
RefSeqiYP_001904110.1. NC_010688.1.

3D structure databases

ProteinModelPortaliB0RUZ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi509169.xccb100_2705.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAP52066; CAP52066; xcc-b100_2705.
GeneIDi6322736.
KEGGixca:xccb100_2705.
PATRICi24085325. VBIXanCam108527_2743.

Phylogenomic databases

eggNOGiNOG77058.
KOiK00452.
OMAiKPPVGNQ.
OrthoDBiEOG6PW234.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00330.
BioCyciXCAM509169:GHW4-2765-MONOMER.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_00825. 3_HAO.
InterProiIPR010329. 3hydroanth_dOase.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis."
    Vorhoelter F.-J., Schneiker S., Goesmann A., Krause L., Bekel T., Kaiser O., Linke B., Patschkowski T., Rueckert C., Schmid J., Sidhu V.K., Sieber V., Tauch A., Watt S.A., Weisshaar B., Becker A., Niehaus K., Puehler A.
    J. Biotechnol. 134:33-45(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B100.

Entry informationi

Entry namei3HAO_XANCB
AccessioniPrimary (citable) accession number: B0RUZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 8, 2008
Last modified: January 7, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.