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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Xanthomonas campestris pv. campestris (strain B100)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei641 – 6411ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciXCAM509169:GHW4-1567-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:xcc-b100_1541
OrganismiXanthomonas campestris pv. campestris (strain B100)
Taxonomic identifieri509169 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
ProteomesiUP000001188 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 885885Leucine--tRNA ligasePRO_0000334838Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB0RR06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 5611"HIGH" regionAdd
BLAST
Motifi638 – 6425"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0RR06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTVEPNAYD PQQVETSAQQ FWDATRAFQV DENSDKPKFY CLSMLPYPSG
60 70 80 90 100
ALHMGHVRNY TISDVVSRYK RMTGHNVLQP MGWDAFGLPA ENAAIKNKTA
110 120 130 140 150
PAKWTYANIE HMRAQLKSLG YAIDWSREFA TCTPDYYVHE QRMFTRLMRK
160 170 180 190 200
GLAYRRNAVV NWDPIDQTVL ANEQVIDGRG WRSGALVEKR EIPQWFLRIT
210 220 230 240 250
DYAQELLDGL DQLDGWPDSV KTMQRNWIGR SEGLEIQFDV RDTTGAALDP
260 270 280 290 300
LRVFTTRPDT LMGVTFVSIA AEHPLAQHAA KSNPELASML ETLKHGGVSE
310 320 330 340 350
AELETQEKRG MATGLTAVHP ISGEEVPVWV ANFVLMGYGT GAVMAVPGHD
360 370 380 390 400
QRDFEFANKY GLPIVQVVKL REPRNDDEQA WDATQWRDWY TDKSRELELI
410 420 430 440 450
NSAEFDGLDY HGAFEALAER FERKGQGQRR INYRLRDWGV SRQRYWGCPI
460 470 480 490 500
PVIYCAKCGA VPVPEDQLPV VLPENVEFAG TGSPIKTDPT WRQTTCPECG
510 520 530 540 550
GPAERETDTF DTFMESSWYV ARYTSPNARD MVDRRANYWM PADLYVGGIE
560 570 580 590 600
HAILHLMYFR FYHKLMRDAR LVDSDEPVTN LLTQGMVIAE TFYRDADNGG
610 620 630 640 650
KDWINPADVE IQRDERGRVV GASLIADGQP VHIGGTEKMS KSKNNGVDPQ
660 670 680 690 700
AMVAKYGADT VRLFSMFAAP PEQSLEWNEA GVDGMARFMR RLWVQVHKHV
710 720 730 740 750
GEGIDDELTK IVALGGPLSA EQKAIRRKTH ETIGKVDDDY GRRHSFNTAI
760 770 780 790 800
AAVMELSNAL AKFDDASAQG RAVRQEALEA MVLLLNPITP HASHALWQVL
810 820 830 840 850
GRGETLLENV PFPQVDAAAL VRDALTLAIQ VNGKLRGTIE VAADAAREQI
860 870 880
EALALAEPNA AKFLDGLSVR KIIIVPGKIV NIVAG
Length:885
Mass (Da):99,140
Last modified:May 20, 2008 - v2
Checksum:iDB659533B2B83BA0
GO

Sequence cautioni

The sequence CAP50891.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM920689 Genomic DNA. Translation: CAP50891.1. Different initiation.

Genome annotation databases

EnsemblBacteriaiCAP50891; CAP50891; xcc-b100_1541.
KEGGixca:xccb100_1541.
PATRICi24082934. VBIXanCam108527_1573.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM920689 Genomic DNA. Translation: CAP50891.1. Different initiation.

3D structure databases

ProteinModelPortaliB0RR06.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAP50891; CAP50891; xcc-b100_1541.
KEGGixca:xccb100_1541.
PATRICi24082934. VBIXanCam108527_1573.

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciXCAM509169:GHW4-1567-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis."
    Vorhoelter F.-J., Schneiker S., Goesmann A., Krause L., Bekel T., Kaiser O., Linke B., Patschkowski T., Rueckert C., Schmid J., Sidhu V.K., Sieber V., Tauch A., Watt S.A., Weisshaar B., Becker A., Niehaus K., Puehler A.
    J. Biotechnol. 134:33-45(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B100.

Entry informationi

Entry nameiSYL_XANCB
AccessioniPrimary (citable) accession number: B0RR06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: July 22, 2015
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.