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B0RP28

- LEU1_XANCB

UniProt

B0RP28 - LEU1_XANCB

Protein

2-isopropylmalate synthase

Gene

leuA

Organism
Xanthomonas campestris pv. campestris (strain B100)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).UniRule annotation

    Catalytic activityi

    Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

    Pathwayi

    GO - Molecular functioni

    1. 2-isopropylmalate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. leucine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

    Enzyme and pathway databases

    BioCyciXCAM509169:GHW4-888-MONOMER.
    UniPathwayiUPA00048; UER00070.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-isopropylmalate synthaseUniRule annotation (EC:2.3.3.13UniRule annotation)
    Alternative name(s):
    Alpha-IPM synthaseUniRule annotation
    Alpha-isopropylmalate synthaseUniRule annotation
    Gene namesi
    Name:leuAUniRule annotation
    Ordered Locus Names:xcc-b100_0870
    OrganismiXanthomonas campestris pv. campestris (strain B100)
    Taxonomic identifieri509169 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
    ProteomesiUP000001188: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5205202-isopropylmalate synthasePRO_1000149334Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi509169.xccb100_0870.

    Structurei

    3D structure databases

    ProteinModelPortaliB0RP28.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0119.
    KOiK01649.
    OMAiEGQNALG.
    OrthoDBiEOG6CGCF3.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01025. LeuA_type1.
    InterProiIPR013709. 2-isopropylmalate_synth_dimer.
    IPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR005671. LeuA_bact_synth.
    IPR000891. PYR_CT.
    [Graphical view]
    PfamiPF00682. HMGL-like. 1 hit.
    PF08502. LeuA_dimer. 1 hit.
    [Graphical view]
    SMARTiSM00917. LeuA_dimer. 1 hit.
    [Graphical view]
    SUPFAMiSSF110921. SSF110921. 1 hit.
    TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
    PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B0RP28-1 [UniParc]FASTAAdd to Basket

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    MNTTVSNQTP HIRIFDTTLR DGEQSPGCSM TPQQKLVMAR ALDALGVDII    50
    ETGFPASSYS DREAVAMMGR ELRRPTLAVL SRCLQADIEI SARALEAAAN 100
    PRLHVFLSTS PLHREHKLRM SREQVLESVH KHVTLARGYI DDIEFSAEDA 150
    TRTEEDFLAE VTRVAIAAGA TTINLPDTVG FTTPEEIRGM FSRLIASVEG 200
    AEKVIFSTHC HNDLGLAAAN SLAAIEGGAR QVECTINGIG ERAGNCALEE 250
    ITMALKVRGA FYNLDTAINT PRIVSTSQLL QRLVGMPVQR NKAVVGGNAF 300
    AHESGIHQHG MLRHRGTYEI MRPEDVGWES SQMVLGRHSG RAAVEQRLRA 350
    LGYLLEEDEA KLVFEQFKAL CEKQRVVTDA DLQALMQDAT VQEGYRLASM 400
    TISDVGSRAN ALVELSDPDG NRVAETAQGN GPVDALFGAL ASATGVKLEL 450
    DSYQVHSVGI GADARGEASL SVRHDGVEYE GTGTSKDIIE ASALAWLDVA 500
    NRLLRQRERG VVAGKTAAVA 520
    Length:520
    Mass (Da):56,222
    Last modified:April 8, 2008 - v1
    Checksum:iA8879B31E2E509D9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM920689 Genomic DNA. Translation: CAP50213.1.
    RefSeqiYP_001902275.1. NC_010688.1.

    Genome annotation databases

    EnsemblBacteriaiCAP50213; CAP50213; xcc-b100_0870.
    GeneIDi6324730.
    KEGGixca:xccb100_0870.
    PATRICi24081569. VBIXanCam108527_0896.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM920689 Genomic DNA. Translation: CAP50213.1 .
    RefSeqi YP_001902275.1. NC_010688.1.

    3D structure databases

    ProteinModelPortali B0RP28.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 509169.xccb100_0870.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAP50213 ; CAP50213 ; xcc-b100_0870 .
    GeneIDi 6324730.
    KEGGi xca:xccb100_0870.
    PATRICi 24081569. VBIXanCam108527_0896.

    Phylogenomic databases

    eggNOGi COG0119.
    KOi K01649.
    OMAi EGQNALG.
    OrthoDBi EOG6CGCF3.

    Enzyme and pathway databases

    UniPathwayi UPA00048 ; UER00070 .
    BioCyci XCAM509169:GHW4-888-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01025. LeuA_type1.
    InterProi IPR013709. 2-isopropylmalate_synth_dimer.
    IPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR005671. LeuA_bact_synth.
    IPR000891. PYR_CT.
    [Graphical view ]
    Pfami PF00682. HMGL-like. 1 hit.
    PF08502. LeuA_dimer. 1 hit.
    [Graphical view ]
    SMARTi SM00917. LeuA_dimer. 1 hit.
    [Graphical view ]
    SUPFAMi SSF110921. SSF110921. 1 hit.
    TIGRFAMsi TIGR00973. leuA_bact. 1 hit.
    PROSITEi PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis."
      Vorhoelter F.-J., Schneiker S., Goesmann A., Krause L., Bekel T., Kaiser O., Linke B., Patschkowski T., Rueckert C., Schmid J., Sidhu V.K., Sieber V., Tauch A., Watt S.A., Weisshaar B., Becker A., Niehaus K., Puehler A.
      J. Biotechnol. 134:33-45(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: B100.

    Entry informationi

    Entry nameiLEU1_XANCB
    AccessioniPrimary (citable) accession number: B0RP28
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3