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B0RP28

- LEU1_XANCB

UniProt

B0RP28 - LEU1_XANCB

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Protein

2-isopropylmalate synthase

Gene

leuA

Organism
Xanthomonas campestris pv. campestris (strain B100)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).UniRule annotation

Catalytic activityi

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-isopropylmalate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

BioCyciXCAM509169:GHW4-888-MONOMER.
UniPathwayiUPA00048; UER00070.

Names & Taxonomyi

Protein namesi
Recommended name:
2-isopropylmalate synthaseUniRule annotation (EC:2.3.3.13UniRule annotation)
Alternative name(s):
Alpha-IPM synthaseUniRule annotation
Alpha-isopropylmalate synthaseUniRule annotation
Gene namesi
Name:leuAUniRule annotation
Ordered Locus Names:xcc-b100_0870
OrganismiXanthomonas campestris pv. campestris (strain B100)
Taxonomic identifieri509169 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
ProteomesiUP000001188: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5205202-isopropylmalate synthasePRO_1000149334Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi509169.xccb100_0870.

Structurei

3D structure databases

ProteinModelPortaliB0RP28.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0119.
KOiK01649.
OMAiEGQNALG.
OrthoDBiEOG6CGCF3.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01025. LeuA_type1.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0RP28-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTTVSNQTP HIRIFDTTLR DGEQSPGCSM TPQQKLVMAR ALDALGVDII
60 70 80 90 100
ETGFPASSYS DREAVAMMGR ELRRPTLAVL SRCLQADIEI SARALEAAAN
110 120 130 140 150
PRLHVFLSTS PLHREHKLRM SREQVLESVH KHVTLARGYI DDIEFSAEDA
160 170 180 190 200
TRTEEDFLAE VTRVAIAAGA TTINLPDTVG FTTPEEIRGM FSRLIASVEG
210 220 230 240 250
AEKVIFSTHC HNDLGLAAAN SLAAIEGGAR QVECTINGIG ERAGNCALEE
260 270 280 290 300
ITMALKVRGA FYNLDTAINT PRIVSTSQLL QRLVGMPVQR NKAVVGGNAF
310 320 330 340 350
AHESGIHQHG MLRHRGTYEI MRPEDVGWES SQMVLGRHSG RAAVEQRLRA
360 370 380 390 400
LGYLLEEDEA KLVFEQFKAL CEKQRVVTDA DLQALMQDAT VQEGYRLASM
410 420 430 440 450
TISDVGSRAN ALVELSDPDG NRVAETAQGN GPVDALFGAL ASATGVKLEL
460 470 480 490 500
DSYQVHSVGI GADARGEASL SVRHDGVEYE GTGTSKDIIE ASALAWLDVA
510 520
NRLLRQRERG VVAGKTAAVA
Length:520
Mass (Da):56,222
Last modified:April 8, 2008 - v1
Checksum:iA8879B31E2E509D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM920689 Genomic DNA. Translation: CAP50213.1.
RefSeqiYP_001902275.1. NC_010688.1.

Genome annotation databases

EnsemblBacteriaiCAP50213; CAP50213; xcc-b100_0870.
GeneIDi6324730.
KEGGixca:xccb100_0870.
PATRICi24081569. VBIXanCam108527_0896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM920689 Genomic DNA. Translation: CAP50213.1 .
RefSeqi YP_001902275.1. NC_010688.1.

3D structure databases

ProteinModelPortali B0RP28.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 509169.xccb100_0870.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAP50213 ; CAP50213 ; xcc-b100_0870 .
GeneIDi 6324730.
KEGGi xca:xccb100_0870.
PATRICi 24081569. VBIXanCam108527_0896.

Phylogenomic databases

eggNOGi COG0119.
KOi K01649.
OMAi EGQNALG.
OrthoDBi EOG6CGCF3.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00070 .
BioCyci XCAM509169:GHW4-888-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01025. LeuA_type1.
InterProi IPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view ]
Pfami PF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view ]
SMARTi SM00917. LeuA_dimer. 1 hit.
[Graphical view ]
SUPFAMi SSF110921. SSF110921. 1 hit.
TIGRFAMsi TIGR00973. leuA_bact. 1 hit.
PROSITEi PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis."
    Vorhoelter F.-J., Schneiker S., Goesmann A., Krause L., Bekel T., Kaiser O., Linke B., Patschkowski T., Rueckert C., Schmid J., Sidhu V.K., Sieber V., Tauch A., Watt S.A., Weisshaar B., Becker A., Niehaus K., Puehler A.
    J. Biotechnol. 134:33-45(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B100.

Entry informationi

Entry nameiLEU1_XANCB
AccessioniPrimary (citable) accession number: B0RP28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 8, 2008
Last modified: October 1, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3