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B0RP28

- LEU1_XANCB

UniProt

B0RP28 - LEU1_XANCB

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Protein
2-isopropylmalate synthase
Gene
leuA, xcc-b100_0870
Organism
Xanthomonas campestris pv. campestris (strain B100)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-isopropylmalate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

BioCyciXCAM509169:GHW4-888-MONOMER.
UniPathwayiUPA00048; UER00070.

Names & Taxonomyi

Protein namesi
Recommended name:
2-isopropylmalate synthase (EC:2.3.3.13)
Alternative name(s):
Alpha-IPM synthase
Alpha-isopropylmalate synthase
Gene namesi
Name:leuA
Ordered Locus Names:xcc-b100_0870
OrganismiXanthomonas campestris pv. campestris (strain B100)
Taxonomic identifieri509169 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
ProteomesiUP000001188: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5205202-isopropylmalate synthaseUniRule annotation
PRO_1000149334Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi509169.xccb100_0870.

Structurei

3D structure databases

ProteinModelPortaliB0RP28.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0119.
KOiK01649.
OMAiEGQNALG.
OrthoDBiEOG6CGCF3.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01025. LeuA_type1.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0RP28-1 [UniParc]FASTAAdd to Basket

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MNTTVSNQTP HIRIFDTTLR DGEQSPGCSM TPQQKLVMAR ALDALGVDII    50
ETGFPASSYS DREAVAMMGR ELRRPTLAVL SRCLQADIEI SARALEAAAN 100
PRLHVFLSTS PLHREHKLRM SREQVLESVH KHVTLARGYI DDIEFSAEDA 150
TRTEEDFLAE VTRVAIAAGA TTINLPDTVG FTTPEEIRGM FSRLIASVEG 200
AEKVIFSTHC HNDLGLAAAN SLAAIEGGAR QVECTINGIG ERAGNCALEE 250
ITMALKVRGA FYNLDTAINT PRIVSTSQLL QRLVGMPVQR NKAVVGGNAF 300
AHESGIHQHG MLRHRGTYEI MRPEDVGWES SQMVLGRHSG RAAVEQRLRA 350
LGYLLEEDEA KLVFEQFKAL CEKQRVVTDA DLQALMQDAT VQEGYRLASM 400
TISDVGSRAN ALVELSDPDG NRVAETAQGN GPVDALFGAL ASATGVKLEL 450
DSYQVHSVGI GADARGEASL SVRHDGVEYE GTGTSKDIIE ASALAWLDVA 500
NRLLRQRERG VVAGKTAAVA 520
Length:520
Mass (Da):56,222
Last modified:April 8, 2008 - v1
Checksum:iA8879B31E2E509D9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM920689 Genomic DNA. Translation: CAP50213.1.
RefSeqiYP_001902275.1. NC_010688.1.

Genome annotation databases

EnsemblBacteriaiCAP50213; CAP50213; xcc-b100_0870.
GeneIDi6324730.
KEGGixca:xccb100_0870.
PATRICi24081569. VBIXanCam108527_0896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM920689 Genomic DNA. Translation: CAP50213.1 .
RefSeqi YP_001902275.1. NC_010688.1.

3D structure databases

ProteinModelPortali B0RP28.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 509169.xccb100_0870.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAP50213 ; CAP50213 ; xcc-b100_0870 .
GeneIDi 6324730.
KEGGi xca:xccb100_0870.
PATRICi 24081569. VBIXanCam108527_0896.

Phylogenomic databases

eggNOGi COG0119.
KOi K01649.
OMAi EGQNALG.
OrthoDBi EOG6CGCF3.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00070 .
BioCyci XCAM509169:GHW4-888-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01025. LeuA_type1.
InterProi IPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view ]
Pfami PF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view ]
SMARTi SM00917. LeuA_dimer. 1 hit.
[Graphical view ]
SUPFAMi SSF110921. SSF110921. 1 hit.
TIGRFAMsi TIGR00973. leuA_bact. 1 hit.
PROSITEi PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis."
    Vorhoelter F.-J., Schneiker S., Goesmann A., Krause L., Bekel T., Kaiser O., Linke B., Patschkowski T., Rueckert C., Schmid J., Sidhu V.K., Sieber V., Tauch A., Watt S.A., Weisshaar B., Becker A., Niehaus K., Puehler A.
    J. Biotechnol. 134:33-45(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B100.

Entry informationi

Entry nameiLEU1_XANCB
AccessioniPrimary (citable) accession number: B0RP28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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