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B0RMR2 (BIOB_XANCB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:xcc-b100_0416
OrganismXanthomonas campestris pv. campestris (strain B100) [Complete proteome] [HAMAP]
Taxonomic identifier509169 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344Biotin synthase HAMAP-Rule MF_01694
PRO_0000381709

Sites

Metal binding551Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding591Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding621Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding991Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1301Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1901Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2621Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B0RMR2 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 4C576AF23C7F7D76

FASTA34437,516
        10         20         30         40         50         60 
MSVVVRHDWD RKELHALFAL PFPELLHRAA SVHRAHFDPA EVQVSTLLSV KTGGCPEDCA 

        70         80         90        100        110        120 
YCPQAQRYDT GVSAQKLMDT EDVVAKARQA KAAGASRFCM GAAWRSPKDR DIPKVAAMIR 

       130        140        150        160        170        180 
EVKAMGLETC ATLGMLDAGQ ARALKDAGLD YYNHNLDTAP DYYDSIIHTR QYQDRLNTLE 

       190        200        210        220        230        240 
HVRDVGLKTC CGGIVGMGET REHRVGLLHA LATLPAHPDS VPINQLVQVP GTPLHGTEPL 

       250        260        270        280        290        300 
DAFEFVRMIA VARIAMPKSM VRLSAGREAM SDELQALCFL AGANSIFYGE KLLTTGNPDT 

       310        320        330        340 
ERDQGLFQRL GLRPMQITVD AAEHDHPGTV HADITCGAAC EHAA 

« Hide

References

[1]"The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis."
Vorhoelter F.-J., Schneiker S., Goesmann A., Krause L., Bekel T., Kaiser O., Linke B., Patschkowski T., Rueckert C., Schmid J., Sidhu V.K., Sieber V., Tauch A., Watt S.A., Weisshaar B., Becker A., Niehaus K., Puehler A.
J. Biotechnol. 134:33-45(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B100.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM920689 Genomic DNA. Translation: CAP49747.1.
RefSeqYP_001901822.1. NC_010688.1.

3D structure databases

ProteinModelPortalB0RMR2.
SMRB0RMR2. Positions 6-316.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING509169.xccb100_0416.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAP49747; CAP49747; xcc-b100_0416.
GeneID6324349.
KEGGxca:xccb100_0416.
PATRIC24080620. VBIXanCam108527_0425.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycXCAM509169:GHW4-419-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_XANCB
AccessionPrimary (citable) accession number: B0RMR2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways