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B0RMR2

- BIOB_XANCB

UniProt

B0RMR2 - BIOB_XANCB

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Protein

Biotin synthase

Gene
bioB, xcc-b100_0416
Organism
Xanthomonas campestris pv. campestris (strain B100)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi99 – 991Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi130 – 1301Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi190 – 1901Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi262 – 2621Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciXCAM509169:GHW4-419-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:xcc-b100_0416
OrganismiXanthomonas campestris pv. campestris (strain B100)
Taxonomic identifieri509169 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
ProteomesiUP000001188: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Biotin synthaseUniRule annotationPRO_0000381709Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi509169.xccb100_0416.

Structurei

3D structure databases

ProteinModelPortaliB0RMR2.
SMRiB0RMR2. Positions 6-316.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B0RMR2-1 [UniParc]FASTAAdd to Basket

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MSVVVRHDWD RKELHALFAL PFPELLHRAA SVHRAHFDPA EVQVSTLLSV    50
KTGGCPEDCA YCPQAQRYDT GVSAQKLMDT EDVVAKARQA KAAGASRFCM 100
GAAWRSPKDR DIPKVAAMIR EVKAMGLETC ATLGMLDAGQ ARALKDAGLD 150
YYNHNLDTAP DYYDSIIHTR QYQDRLNTLE HVRDVGLKTC CGGIVGMGET 200
REHRVGLLHA LATLPAHPDS VPINQLVQVP GTPLHGTEPL DAFEFVRMIA 250
VARIAMPKSM VRLSAGREAM SDELQALCFL AGANSIFYGE KLLTTGNPDT 300
ERDQGLFQRL GLRPMQITVD AAEHDHPGTV HADITCGAAC EHAA 344
Length:344
Mass (Da):37,516
Last modified:May 20, 2008 - v1
Checksum:i4C576AF23C7F7D76
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM920689 Genomic DNA. Translation: CAP49747.1.
RefSeqiYP_001901822.1. NC_010688.1.

Genome annotation databases

EnsemblBacteriaiCAP49747; CAP49747; xcc-b100_0416.
GeneIDi6324349.
KEGGixca:xccb100_0416.
PATRICi24080620. VBIXanCam108527_0425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM920689 Genomic DNA. Translation: CAP49747.1 .
RefSeqi YP_001901822.1. NC_010688.1.

3D structure databases

ProteinModelPortali B0RMR2.
SMRi B0RMR2. Positions 6-316.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 509169.xccb100_0416.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAP49747 ; CAP49747 ; xcc-b100_0416 .
GeneIDi 6324349.
KEGGi xca:xccb100_0416.
PATRICi 24080620. VBIXanCam108527_0425.

Phylogenomic databases

eggNOGi COG0502.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci XCAM509169:GHW4-419-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis."
    Vorhoelter F.-J., Schneiker S., Goesmann A., Krause L., Bekel T., Kaiser O., Linke B., Patschkowski T., Rueckert C., Schmid J., Sidhu V.K., Sieber V., Tauch A., Watt S.A., Weisshaar B., Becker A., Niehaus K., Puehler A.
    J. Biotechnol. 134:33-45(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B100.

Entry informationi

Entry nameiBIOB_XANCB
AccessioniPrimary (citable) accession number: B0RMR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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