Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

B0RIP8

- SYE_CLAMS

UniProt

B0RIP8 - SYE_CLAMS

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (08 Apr 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei270 – 2701ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCMIC31964:GJBN-1436-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:CMS1428
    OrganismiClavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
    Taxonomic identifieri31964 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter
    ProteomesiUP000001318: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 506506Glutamate--tRNA ligasePRO_1000074318Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi31964.CMS_1428.

    Structurei

    3D structure databases

    ProteinModelPortaliB0RIP8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi23 – 3311"HIGH" regionAdd
    BLAST
    Motifi267 – 2715"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    KOiK01885.
    OMAiAMGWEVP.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B0RIP8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTETTAHPVT TATGTDVRVR FCPSPTGTPH VGLIRTALFN WAYARHTGGK    50
    LVFRVEDTDA ARDSEESYEQ LIEALRWLEI DWDEGEGVGG PHAPYRQSQR 100
    TDLYLDVIAK LTASGHLYES FATAEEIEAR NRAAGRDPKM GYDNFERDLT 150
    EEERQAFRDE GRSPALRLRV PDTDLSFDDL VRGTVTFPAG SFPDFVLVRP 200
    NGAPLYTLVN PVDDALMGIT HVLRGEDLLS STPRQIALYH ALIDIGVADA 250
    IPRFGHLPYV MGEGNKKLSK RDPESNLFHH RDRGFIPEGL INYLALLGWS 300
    LTHDRDVFSR MEMVTAFDVA DVNPNPARFD LKKAESLNGD HIRLLALDDF 350
    AQRLVPYLQA ADVVGAELTH DQRRMLEAAA PLVQERMQLL GEAPDLLSFL 400
    FTTADELPYD DAAVQALKDD APEVLAASRG ALSGVPHTQW DIDLVQEVLQ 450
    NTLITGMGMK PRLAYGPLRV GISGRRISPP LFESMVLLGK DETIARLDRL 500
    AGMLGE 506
    Length:506
    Mass (Da):56,148
    Last modified:April 8, 2008 - v1
    Checksum:iD9316DC3517A73A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM849034 Genomic DNA. Translation: CAQ01539.1.
    RefSeqiYP_001710155.1. NC_010407.1.

    Genome annotation databases

    EnsemblBacteriaiCAQ01539; CAQ01539; CMS1428.
    GeneIDi6157258.
    KEGGicms:CMS_1428.
    PATRICi21459870. VBIClaMic4666_1492.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM849034 Genomic DNA. Translation: CAQ01539.1 .
    RefSeqi YP_001710155.1. NC_010407.1.

    3D structure databases

    ProteinModelPortali B0RIP8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 31964.CMS_1428.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAQ01539 ; CAQ01539 ; CMS1428 .
    GeneIDi 6157258.
    KEGGi cms:CMS_1428.
    PATRICi 21459870. VBIClaMic4666_1492.

    Phylogenomic databases

    eggNOGi COG0008.
    KOi K01885.
    OMAi AMGWEVP.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci CMIC31964:GJBN-1436-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation."
      Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A.
      J. Bacteriol. 190:2150-2160(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1.

    Entry informationi

    Entry nameiSYE_CLAMS
    AccessioniPrimary (citable) accession number: B0RIP8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3