B0RIP8 (SYE_CLAMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	CMS1428

Organism

Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / JCM 9667) [Complete proteome] [HAMAP]

Taxonomic identifier

31964 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000074318
Regions
	Motif	23 – 33	11	"HIGH" region HAMAP MF_00022_B
	Motif	267 – 271	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	270	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0RIP8 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: D9316DC3517A73A2	FASTA	506	56,148
	10 20 30 40 50 60 MTETTAHPVT TATGTDVRVR FCPSPTGTPH VGLIRTALFN WAYARHTGGK LVFRVEDTDA 70 80 90 100 110 120 ARDSEESYEQ LIEALRWLEI DWDEGEGVGG PHAPYRQSQR TDLYLDVIAK LTASGHLYES 130 140 150 160 170 180 FATAEEIEAR NRAAGRDPKM GYDNFERDLT EEERQAFRDE GRSPALRLRV PDTDLSFDDL 190 200 210 220 230 240 VRGTVTFPAG SFPDFVLVRP NGAPLYTLVN PVDDALMGIT HVLRGEDLLS STPRQIALYH 250 260 270 280 290 300 ALIDIGVADA IPRFGHLPYV MGEGNKKLSK RDPESNLFHH RDRGFIPEGL INYLALLGWS 310 320 330 340 350 360 LTHDRDVFSR MEMVTAFDVA DVNPNPARFD LKKAESLNGD HIRLLALDDF AQRLVPYLQA 370 380 390 400 410 420 ADVVGAELTH DQRRMLEAAA PLVQERMQLL GEAPDLLSFL FTTADELPYD DAAVQALKDD 430 440 450 460 470 480 APEVLAASRG ALSGVPHTQW DIDLVQEVLQ NTLITGMGMK PRLAYGPLRV GISGRRISPP 490 500 LFESMVLLGK DETIARLDRL AGMLGE

References

[1]

"Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation." Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A. J. Bacteriol. 190:2150-2160(2008) [PubMed: 18192393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33113 / JCM 9667.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM849034 Genomic DNA. Translation: CAQ01539.1.
RefSeq	YP_001710155.1. NC_010407.1.
3D structure databases
ProteinModelPortal	B0RIP8.
ModBase	Search...
Protein-protein interaction databases
STRING	B0RIP8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6157258.
GenomeReviews	Gene locus CMS1428 in contig AM849034_GR.
KEGG	cms:CMS_1428.
PATRIC	21459870. VBIClaMic4666_1492.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	MAHIPLI.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	CMIC31964:CMS1428-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_CLAMS

Accession

Primary (citable) accession number: B0RIP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program