B0RI49 (RSMH_CLAMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	CMS1365

Organism

Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / JCM 9667) [Complete proteome] [HAMAP]

Taxonomic identifier

31964 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000386808
Regions
	Region	38 – 40	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	57	1	S-adenosyl-L-methionine By similarity
	Binding site	91	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	105	1	S-adenosyl-L-methionine By similarity
	Binding site	112	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0RI49 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: D00F8C7B85682990	FASTA	318	34,817
	10 20 30 40 50 60 MALDDIHTPV LLERCLELLA PALQGEGAVL VDATLGMAGH SEAFLDALPG LRLVGLDRDP 70 80 90 100 110 120 DALAIAGERL ARFGDRVHLV HTVYDGIGRA LDGLGIGEVQ GVFFDLGVSS LQLDRVERGF 130 140 150 160 170 180 SYSQDAPLDM RMDGTAGLTA AQVVAEYDEL ELRRIFYDYG EEKLAPRYAS RIVQAREVEP 190 200 210 220 230 240 ITTSARLVEI IQQATPAAVQ RAGHPAKRVF QALRIEVNQE LSVLARAMPA AIDRLAVGGR 250 260 270 280 290 300 VVVESYQSLE DRIVKRELRV RSTSTAPVGL PVELPEHRPE LKLLVRGAEL ADQHEIAQNP 310 RAASVRLRAA ERARRRHA

References

[1]

"Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation." Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A. J. Bacteriol. 190:2150-2160(2008) [PubMed: 18192393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33113 / JCM 9667.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM849034 Genomic DNA. Translation: CAQ01476.1.
RefSeq	YP_001710092.1. NC_010407.1.
3D structure databases
ProteinModelPortal	B0RI49.
ModBase	Search...
Protein-protein interaction databases
STRING	B0RI49.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6158823.
GenomeReviews	Gene locus CMS1365 in contig AM849034_GR.
KEGG	cms:CMS_1365.
PATRIC	21459737. VBIClaMic4666_1426.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG302779.
OMA	NPATRAF.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	CMIC31964:CMS1365-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_CLAMS

Accession

Primary (citable) accession number: B0RI49

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program