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Protein

Argininosuccinate synthase

Gene

argG

Organism
Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87CitrullineUniRule annotation1
Binding sitei117ATP; via amide nitrogenUniRule annotation1
Binding sitei119AspartateUniRule annotation1
Binding sitei123AspartateUniRule annotation1
Binding sitei123CitrullineUniRule annotation1
Binding sitei124AspartateUniRule annotation1
Binding sitei127CitrullineUniRule annotation1
Binding sitei175CitrullineUniRule annotation1
Binding sitei259CitrullineUniRule annotation1
Binding sitei271CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 16ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:CMS1236
OrganismiClavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Taxonomic identifieri31964 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrobacteriaceaeClavibacter
Proteomesi
  • UP000001318 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000738161 – 417Argininosuccinate synthaseAdd BLAST417

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi31964.CMS_1236.

Structurei

3D structure databases

ProteinModelPortaliB0RHD5.
SMRiB0RHD5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
KOiK01940.
OMAiQCEVVTF.
OrthoDBiPOG091H01U0.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0RHD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAERVVLAYS GGLDTSVGIG WLKDATGKEV VALAVDVGQG GEDMEVIRQR
60 70 80 90 100
ALDCGAVEAV VVDAKDEFAD DYIVPALKAN ALYQKRYPLV SGLSRPLIAK
110 120 130 140 150
HLARVAHELG ANSVAHGCTG KGNDQVRFEA AVAALAPDLT SIAPVRDLAL
160 170 180 190 200
TRDKAIVYAN EHDLPIEQSK KSPYSIDKNV WGRAVETGFL EDPWNGPIED
210 220 230 240 250
LYEYTQDPDV LREATEVTIT FEAGVPVAID GIRYSPLRIV QELNAAAGAH
260 270 280 290 300
GIGRIDVVED RLVGIKSREV YEAPAAMTLI EAHEELEALT IERDLGRYKR
310 320 330 340 350
GVEKDWANLV YDGLWFSGLK RSLDAFIEDS QRHVSGDIRM TLRGGRAVVT
360 370 380 390 400
GRRSETSLYD FDLATYDTGD TFDQSLSKGF IELWSLPSKI SARRDLAVEQ
410
AALAADDATP AAAPAAE
Length:417
Mass (Da):45,281
Last modified:April 8, 2008 - v1
Checksum:i72C8C9211BC76BCE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM849034 Genomic DNA. Translation: CAQ01352.1.
RefSeqiWP_012298630.1. NC_010407.1.

Genome annotation databases

EnsemblBacteriaiCAQ01352; CAQ01352; CMS1236.
KEGGicms:CMS1236.
PATRICi21459472. VBIClaMic4666_1298.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM849034 Genomic DNA. Translation: CAQ01352.1.
RefSeqiWP_012298630.1. NC_010407.1.

3D structure databases

ProteinModelPortaliB0RHD5.
SMRiB0RHD5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi31964.CMS_1236.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAQ01352; CAQ01352; CMS1236.
KEGGicms:CMS1236.
PATRICi21459472. VBIClaMic4666_1298.

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
KOiK01940.
OMAiQCEVVTF.
OrthoDBiPOG091H01U0.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_CLAMS
AccessioniPrimary (citable) accession number: B0RHD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: November 2, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.