B0RHC8 (B0RHC8_CLAMS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281
EC=6.1.1.20 HAMAP MF_00281

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281

Gene names

Name:	pheS HAMAP MF_00281
Ordered Locus Names:	CMS1229

Organism

Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / JCM 9667) [Complete proteome] [HAMAP] EMBL CAQ01345.1

Taxonomic identifier

31964 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity. HAMAP MF_00281
Subcellular location	Cytoplasm By similarity HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily. HAMAP MF_00281

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00281
Cellular component	Cytoplasm HAMAP MF_00281
Ligand	ATP-binding HAMAP MF_00281 Magnesium HAMAP MF_00281 Metal-binding HAMAP MF_00281 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00281 EMBL CAQ01345.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B0RHC8 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 53286DC8A7422283	FASTA	345	38,149
	10 20 30 40 50 60 MSESQISEET VGAAVEQAMA ALAATTDSAS LAQARSAHIG EASPLARLNG SLRSLPPEQR 70 80 90 100 110 120 KDAGKLVGQS RARVTQAFQA REAEIQEQEA AERLVAEAVD VTALPSRWRA GARHPLTMLE 130 140 150 160 170 180 ERIADIFVGM GWQVNEGPEL ESEWFNFDAL NFPPDHPARA MQDSFYVDPT DAHLVLRTHT 190 200 210 220 230 240 SPVQIRTLLA DELPVYTIAQ GRTFRSDELD ATHTPAFRQI EGIAIDRGLT MAHLRGTLEH 250 260 270 280 290 300 FARSMFGREA RVRLRPNYFP FTEPSAEMDV WHPAAAGGPR WIEWGGCGMV NPNVLRSAGI 310 320 330 340 DPDEYQGFAF GMGTERTLMF RNDLSDMRDI VEGDIRFGAQ FGMVV

References

[1]

"Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation." Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A. J. Bacteriol. 190:2150-2160(2008) [PubMed: 18192393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM849034 Genomic DNA. Translation: CAQ01345.1.
RefSeq	YP_001709967.1. NC_010407.1.
3D structure databases
ProteinModelPortal	B0RHC8.
ModBase	Search...
Protein-protein interaction databases
STRING	B0RHC8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6157105.
GenomeReviews	Gene locus CMS1229 in contig AM849034_GR.
KEGG	cms:CMS_1229.
PATRIC	21459458. VBIClaMic4666_1291.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284353.
OMA	FRASYFP.
ProtClustDB	PRK00488.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B0RHC8_CLAMS

Accession

Primary (citable) accession number: B0RHC8

Entry history

Integrated into UniProtKB/TrEMBL:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)