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B0RHB0

- DAPF_CLAMS

UniProt

B0RHB0 - DAPF_CLAMS

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Protein

Diaminopimelate epimerase

Gene
dapF, CMS1212
Organism
Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141Substrate By similarity
Binding sitei78 – 781Substrate By similarity
Active sitei87 – 871Proton donor/acceptor By similarity
Binding sitei164 – 1641Substrate By similarity
Sitei166 – 1661Important for catalytic activity By similarity
Binding sitei197 – 1971Substrate By similarity
Sitei221 – 2211Important for catalytic activity By similarity
Active sitei230 – 2301Proton donor/acceptor By similarity

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciCMIC31964:GJBN-1220-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimerase (EC:5.1.1.7)
Short name:
DAP epimerase
Gene namesi
Name:dapF
Ordered Locus Names:CMS1212
OrganismiClavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Taxonomic identifieri31964 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter
ProteomesiUP000001318: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292Diaminopimelate epimeraseUniRule annotationPRO_1000077694Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi87 ↔ 230UniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi31964.CMS_1212.

Structurei

3D structure databases

ProteinModelPortaliB0RHB0.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 893Substrate binding By similarity
Regioni221 – 2222Substrate binding By similarity
Regioni231 – 2322Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0253.
KOiK01778.
OMAiAIRHWAG.
OrthoDBiEOG6ND0M5.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0RHB0-1 [UniParc]FASTAAdd to Basket

« Hide

MADLQFTKGQ GTGNDFVLFA DPAGEIDLTD TQVQALCDRH FGIGADGTIR    50
AVLSSRIPEG RAALDEDPDA EWFMDYRNVD GSPAEMCGNG IRVFTLFLIE 100
NGLIELPPGR TVPIGTRAGV RDVQRSGSGF QVDLGRWALA GGEPLVRAKD 150
LQVARPGLGI DVGNPHVVVA LSSEDELAEA DLAFAPQLDP EPAEGANVEL 200
VVPADPLIVD GVGHITMRVH ERGSGETLSC GTGAAAAALA IRHWAGAAAP 250
HQWRVQLPGG VLGVRMFPTE DGEHVGLSGP AELVFDGVVA LA 292
Length:292
Mass (Da):30,562
Last modified:April 8, 2008 - v1
Checksum:i9AC0AEF004FE3313
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM849034 Genomic DNA. Translation: CAQ01327.1.
RefSeqiWP_012298605.1. NC_010407.1.
YP_001709949.1. NC_010407.1.

Genome annotation databases

EnsemblBacteriaiCAQ01327; CAQ01327; CMS1212.
GeneIDi6157094.
KEGGicms:CMS_1212.
PATRICi21459422. VBIClaMic4666_1273.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM849034 Genomic DNA. Translation: CAQ01327.1 .
RefSeqi WP_012298605.1. NC_010407.1.
YP_001709949.1. NC_010407.1.

3D structure databases

ProteinModelPortali B0RHB0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 31964.CMS_1212.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAQ01327 ; CAQ01327 ; CMS1212 .
GeneIDi 6157094.
KEGGi cms:CMS_1212.
PATRICi 21459422. VBIClaMic4666_1273.

Phylogenomic databases

eggNOGi COG0253.
KOi K01778.
OMAi AIRHWAG.
OrthoDBi EOG6ND0M5.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci CMIC31964:GJBN-1220-MONOMER.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation."
    Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A.
    J. Bacteriol. 190:2150-2160(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1.

Entry informationi

Entry nameiDAPF_CLAMS
AccessioniPrimary (citable) accession number: B0RHB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: September 3, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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