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B0RH21 (B0RH21_CLAMS) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191
EC=1.17.1.2 HAMAP MF_00191
Gene names
Name:ispH HAMAP MF_00191
Ordered Locus Names:CMS2415
OrganismClavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / JCM 9667) [Complete proteome] [HAMAP] EMBL CAQ02497.1
Taxonomic identifier31964 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191

Catalytic activity

Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191

Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191

Cofactor

Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191

Sequence similarities

Belongs to the IspH family. HAMAP MF_00191

Sequences

Sequence LengthMass (Da)Tools
B0RH21 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: DB4A3BA9F85AA632

FASTA35337,927
        10         20         30         40         50         60 
MGAPVVSLSM PRMPGVRNRL KDNPVAGPKK VLLAAPRGYC AGVDRAVVAV EKALERYGAP 

        70         80         90        100        110        120 
VYVRKQIVHN VHVVSTLERM GAIFVEEVDE VPEGAHVVFS AHGVSPAVVQ GAADRGLQAI 

       130        140        150        160        170        180 
DATCPLVTKV HREAVRFAKA DMQILLIGHE GHEEVEGTAG EAPEQTIVVN SPEHADVIEV 

       190        200        210        220        230        240 
KDPDNLVWLS QTTLSVDETM ETVRRLRARF PNLQDPPSDD ICYATQNRQV AIKKVAVDAD 

       250        260        270        280        290        300 
LVIVIGSANS SNSVRLVEVA LEYGAKASYR VDYASEVKQE WLDGVNTVGV TSGASVPEVL 

       310        320        330        340        350 
VQELLDDLAD AGYGDVTAVV TAEEDLVFSL PKELRKDQSG NTDSRAIGGR TRA

« Hide

References

[1]"Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation."
Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A.
J. Bacteriol. 190:2150-2160(2008) [PubMed: 18192393] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM849034 Genomic DNA. Translation: CAQ02497.1.
RefSeqYP_001711074.1. NC_010407.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGB0RH21.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6158014.
GenomeReviewsGene locus CMS2415 in contig AM849034_GR.
KEGGcms:CMS_2415.
PATRIC21461861. VBIClaMic4666_2474.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG335228.
OMAAMAPECD.
ProtClustDBPRK01045.

Family and domain databases

HAMAPMF_00191. IspH.
[Tree]
InterProIPR003451. LytB.
[Graphical view]
KOK03527.
PfamPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00216. IspH_lytB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB0RH21_CLAMS
AccessionPrimary (citable) accession number: B0RH21
Entry history
Integrated into UniProtKB/TrEMBL: April 8, 2008
Last sequence update: April 8, 2008
Last modified: December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info