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B0RGW7

- PNP_CLAMS

UniProt

B0RGW7 - PNP_CLAMS

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi525 – 5251MagnesiumUniRule annotation
    Metal bindingi531 – 5311MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciCMIC31964:GJBN-1194-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:CMS1186
    OrganismiClavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
    Taxonomic identifieri31964 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter
    ProteomesiUP000001318: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 757757Polyribonucleotide nucleotidyltransferasePRO_0000381876Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi31964.CMS_1186.

    Structurei

    3D structure databases

    ProteinModelPortaliB0RGW7.
    SMRiB0RGW7. Positions 5-728.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini591 – 65060KHUniRule annotationAdd
    BLAST
    Domaini662 – 73473S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    KOiK00962.
    OMAiPRWDWVA.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR014069. pppGpp_PNP.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    TIGR02696. pppGpp_PNP. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B0RGW7-1 [UniParc]FASTAAdd to Basket

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    MEGPEIKFAE AVLDNGKYGT RTVRFEAGRL AQQAQGAVAA YLDEDTMLLS    50
    ATSVGKHPKD NFDFFPLTID VEERSYAAGK IPGSFFRREG RPSTEAILVC 100
    RLIDRPLRPS FITGLRNEVQ VVITVLSIAP DEFYDSLAIN AASASSMLSG 150
    IPFSGPIAGV RLALIGDQWV VFPKHSQLKE AVFDITVAGR VVTDAQGNED 200
    VAIMMVEAEA TEGAWDLIQA GATKPDEAVV AQGLEAAKPF IRQLVAAQAS 250
    LAQQAAKPTV DYPVFLPYAQ QSYDAVSALA LEELGTVYRI ADKIERQDAD 300
    DALKTRTKEA VAAKVEAGEL PQSALTEFSA AYKSVTKTVV RGRILRDGIR 350
    MDGRGLADIR PLDAEVQVIP RVHGSAIFQR GETQILGVTT LNMLKMEQQI 400
    DSLSPVTKKR FMTHYNFPPY STGETGRVGS PKRREIGHGF LAERALVPVL 450
    PSREDFPYAI RQVSEALGSN GSTSMGSVCA STLSLLNAGV PLRAPVAGIA 500
    MGLVSDTVDG QVRYAALTDI LGAEDALGDM DFKVAGTSEF VTAIQLDTKL 550
    DGIPTSVLDG ALKQAKEART AILGVLNQAI DAPDEMAPTA PRVISVNIPV 600
    DKIGELIGPK GKTINAIQDE TGADISIEED GAVYIGAVDG PSAEAARAQV 650
    NAIANPTNPE VGESFLGTVV KIATFGAFVS LLPGKDGLLH ISEVRKLAGG 700
    KRVENVEDVL GVGQKILVEI TKIDDRGKLS LAPVMEEAAD QEGSAAASDG 750
    PEAPAEG 757
    Length:757
    Mass (Da):80,257
    Last modified:April 8, 2008 - v1
    Checksum:i5AD9D620E4A268E7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM849034 Genomic DNA. Translation: CAQ01301.1.
    RefSeqiWP_012298580.1. NC_010407.1.
    YP_001709924.1. NC_010407.1.

    Genome annotation databases

    EnsemblBacteriaiCAQ01301; CAQ01301; CMS1186.
    GeneIDi6157071.
    KEGGicms:CMS_1186.
    PATRICi21459368. VBIClaMic4666_1246.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM849034 Genomic DNA. Translation: CAQ01301.1 .
    RefSeqi WP_012298580.1. NC_010407.1.
    YP_001709924.1. NC_010407.1.

    3D structure databases

    ProteinModelPortali B0RGW7.
    SMRi B0RGW7. Positions 5-728.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 31964.CMS_1186.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAQ01301 ; CAQ01301 ; CMS1186 .
    GeneIDi 6157071.
    KEGGi cms:CMS_1186.
    PATRICi 21459368. VBIClaMic4666_1246.

    Phylogenomic databases

    eggNOGi COG1185.
    KOi K00962.
    OMAi PRWDWVA.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci CMIC31964:GJBN-1194-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR014069. pppGpp_PNP.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    TIGR02696. pppGpp_PNP. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation."
      Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A.
      J. Bacteriol. 190:2150-2160(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1.

    Entry informationi

    Entry nameiPNP_CLAMS
    AccessioniPrimary (citable) accession number: B0RGW7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3