SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B0RGW7

- PNP_CLAMS

UniProt

B0RGW7 - PNP_CLAMS

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, CMS1186
Organism
Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi525 – 5251Magnesium By similarity
Metal bindingi531 – 5311Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCMIC31964:GJBN-1194-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:CMS1186
OrganismiClavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Taxonomic identifieri31964 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter
ProteomesiUP000001318: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_0000381876Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi31964.CMS_1186.

Structurei

3D structure databases

ProteinModelPortaliB0RGW7.
SMRiB0RGW7. Positions 5-728.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini591 – 65060KH
Add
BLAST
Domaini662 – 73473S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0RGW7-1 [UniParc]FASTAAdd to Basket

« Hide

MEGPEIKFAE AVLDNGKYGT RTVRFEAGRL AQQAQGAVAA YLDEDTMLLS    50
ATSVGKHPKD NFDFFPLTID VEERSYAAGK IPGSFFRREG RPSTEAILVC 100
RLIDRPLRPS FITGLRNEVQ VVITVLSIAP DEFYDSLAIN AASASSMLSG 150
IPFSGPIAGV RLALIGDQWV VFPKHSQLKE AVFDITVAGR VVTDAQGNED 200
VAIMMVEAEA TEGAWDLIQA GATKPDEAVV AQGLEAAKPF IRQLVAAQAS 250
LAQQAAKPTV DYPVFLPYAQ QSYDAVSALA LEELGTVYRI ADKIERQDAD 300
DALKTRTKEA VAAKVEAGEL PQSALTEFSA AYKSVTKTVV RGRILRDGIR 350
MDGRGLADIR PLDAEVQVIP RVHGSAIFQR GETQILGVTT LNMLKMEQQI 400
DSLSPVTKKR FMTHYNFPPY STGETGRVGS PKRREIGHGF LAERALVPVL 450
PSREDFPYAI RQVSEALGSN GSTSMGSVCA STLSLLNAGV PLRAPVAGIA 500
MGLVSDTVDG QVRYAALTDI LGAEDALGDM DFKVAGTSEF VTAIQLDTKL 550
DGIPTSVLDG ALKQAKEART AILGVLNQAI DAPDEMAPTA PRVISVNIPV 600
DKIGELIGPK GKTINAIQDE TGADISIEED GAVYIGAVDG PSAEAARAQV 650
NAIANPTNPE VGESFLGTVV KIATFGAFVS LLPGKDGLLH ISEVRKLAGG 700
KRVENVEDVL GVGQKILVEI TKIDDRGKLS LAPVMEEAAD QEGSAAASDG 750
PEAPAEG 757
Length:757
Mass (Da):80,257
Last modified:April 8, 2008 - v1
Checksum:i5AD9D620E4A268E7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM849034 Genomic DNA. Translation: CAQ01301.1.
RefSeqiWP_012298580.1. NC_010407.1.
YP_001709924.1. NC_010407.1.

Genome annotation databases

EnsemblBacteriaiCAQ01301; CAQ01301; CMS1186.
GeneIDi6157071.
KEGGicms:CMS_1186.
PATRICi21459368. VBIClaMic4666_1246.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM849034 Genomic DNA. Translation: CAQ01301.1 .
RefSeqi WP_012298580.1. NC_010407.1.
YP_001709924.1. NC_010407.1.

3D structure databases

ProteinModelPortali B0RGW7.
SMRi B0RGW7. Positions 5-728.
ModBasei Search...

Protein-protein interaction databases

STRINGi 31964.CMS_1186.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAQ01301 ; CAQ01301 ; CMS1186 .
GeneIDi 6157071.
KEGGi cms:CMS_1186.
PATRICi 21459368. VBIClaMic4666_1246.

Phylogenomic databases

eggNOGi COG1185.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci CMIC31964:GJBN-1194-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation."
    Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A.
    J. Bacteriol. 190:2150-2160(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1.

Entry informationi

Entry nameiPNP_CLAMS
AccessioniPrimary (citable) accession number: B0RGW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: April 8, 2008
Last modified: September 3, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi