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B0RGW7

- PNP_CLAMS

UniProt

B0RGW7 - PNP_CLAMS

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi525 – 5251MagnesiumUniRule annotation
Metal bindingi531 – 5311MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCMIC31964:GJBN-1194-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:CMS1186
OrganismiClavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Taxonomic identifieri31964 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter
ProteomesiUP000001318: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757Polyribonucleotide nucleotidyltransferasePRO_0000381876Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi31964.CMS_1186.

Structurei

3D structure databases

ProteinModelPortaliB0RGW7.
SMRiB0RGW7. Positions 5-728.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini591 – 65060KHUniRule annotationAdd
BLAST
Domaini662 – 73473S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0RGW7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEGPEIKFAE AVLDNGKYGT RTVRFEAGRL AQQAQGAVAA YLDEDTMLLS
60 70 80 90 100
ATSVGKHPKD NFDFFPLTID VEERSYAAGK IPGSFFRREG RPSTEAILVC
110 120 130 140 150
RLIDRPLRPS FITGLRNEVQ VVITVLSIAP DEFYDSLAIN AASASSMLSG
160 170 180 190 200
IPFSGPIAGV RLALIGDQWV VFPKHSQLKE AVFDITVAGR VVTDAQGNED
210 220 230 240 250
VAIMMVEAEA TEGAWDLIQA GATKPDEAVV AQGLEAAKPF IRQLVAAQAS
260 270 280 290 300
LAQQAAKPTV DYPVFLPYAQ QSYDAVSALA LEELGTVYRI ADKIERQDAD
310 320 330 340 350
DALKTRTKEA VAAKVEAGEL PQSALTEFSA AYKSVTKTVV RGRILRDGIR
360 370 380 390 400
MDGRGLADIR PLDAEVQVIP RVHGSAIFQR GETQILGVTT LNMLKMEQQI
410 420 430 440 450
DSLSPVTKKR FMTHYNFPPY STGETGRVGS PKRREIGHGF LAERALVPVL
460 470 480 490 500
PSREDFPYAI RQVSEALGSN GSTSMGSVCA STLSLLNAGV PLRAPVAGIA
510 520 530 540 550
MGLVSDTVDG QVRYAALTDI LGAEDALGDM DFKVAGTSEF VTAIQLDTKL
560 570 580 590 600
DGIPTSVLDG ALKQAKEART AILGVLNQAI DAPDEMAPTA PRVISVNIPV
610 620 630 640 650
DKIGELIGPK GKTINAIQDE TGADISIEED GAVYIGAVDG PSAEAARAQV
660 670 680 690 700
NAIANPTNPE VGESFLGTVV KIATFGAFVS LLPGKDGLLH ISEVRKLAGG
710 720 730 740 750
KRVENVEDVL GVGQKILVEI TKIDDRGKLS LAPVMEEAAD QEGSAAASDG

PEAPAEG
Length:757
Mass (Da):80,257
Last modified:April 8, 2008 - v1
Checksum:i5AD9D620E4A268E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM849034 Genomic DNA. Translation: CAQ01301.1.
RefSeqiYP_001709924.1. NC_010407.1.

Genome annotation databases

EnsemblBacteriaiCAQ01301; CAQ01301; CMS1186.
GeneIDi6157071.
KEGGicms:CMS_1186.
PATRICi21459368. VBIClaMic4666_1246.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM849034 Genomic DNA. Translation: CAQ01301.1 .
RefSeqi YP_001709924.1. NC_010407.1.

3D structure databases

ProteinModelPortali B0RGW7.
SMRi B0RGW7. Positions 5-728.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 31964.CMS_1186.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAQ01301 ; CAQ01301 ; CMS1186 .
GeneIDi 6157071.
KEGGi cms:CMS_1186.
PATRICi 21459368. VBIClaMic4666_1246.

Phylogenomic databases

eggNOGi COG1185.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci CMIC31964:GJBN-1194-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation."
    Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A.
    J. Bacteriol. 190:2150-2160(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1.

Entry informationi

Entry nameiPNP_CLAMS
AccessioniPrimary (citable) accession number: B0RGW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: April 8, 2008
Last modified: November 26, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3