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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi525MagnesiumUniRule annotation1
Metal bindingi531MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:CMS1186
OrganismiClavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Taxonomic identifieri31964 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrobacteriaceaeClavibacter
Proteomesi
  • UP000001318 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003818761 – 757Polyribonucleotide nucleotidyltransferaseAdd BLAST757

Proteomic databases

PRIDEiB0RGW7.

Interactioni

Protein-protein interaction databases

STRINGi31964.CMS_1186.

Structurei

3D structure databases

ProteinModelPortaliB0RGW7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini591 – 650KHUniRule annotationAdd BLAST60
Domaini662 – 734S1 motifUniRule annotationAdd BLAST73

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0RGW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGPEIKFAE AVLDNGKYGT RTVRFEAGRL AQQAQGAVAA YLDEDTMLLS
60 70 80 90 100
ATSVGKHPKD NFDFFPLTID VEERSYAAGK IPGSFFRREG RPSTEAILVC
110 120 130 140 150
RLIDRPLRPS FITGLRNEVQ VVITVLSIAP DEFYDSLAIN AASASSMLSG
160 170 180 190 200
IPFSGPIAGV RLALIGDQWV VFPKHSQLKE AVFDITVAGR VVTDAQGNED
210 220 230 240 250
VAIMMVEAEA TEGAWDLIQA GATKPDEAVV AQGLEAAKPF IRQLVAAQAS
260 270 280 290 300
LAQQAAKPTV DYPVFLPYAQ QSYDAVSALA LEELGTVYRI ADKIERQDAD
310 320 330 340 350
DALKTRTKEA VAAKVEAGEL PQSALTEFSA AYKSVTKTVV RGRILRDGIR
360 370 380 390 400
MDGRGLADIR PLDAEVQVIP RVHGSAIFQR GETQILGVTT LNMLKMEQQI
410 420 430 440 450
DSLSPVTKKR FMTHYNFPPY STGETGRVGS PKRREIGHGF LAERALVPVL
460 470 480 490 500
PSREDFPYAI RQVSEALGSN GSTSMGSVCA STLSLLNAGV PLRAPVAGIA
510 520 530 540 550
MGLVSDTVDG QVRYAALTDI LGAEDALGDM DFKVAGTSEF VTAIQLDTKL
560 570 580 590 600
DGIPTSVLDG ALKQAKEART AILGVLNQAI DAPDEMAPTA PRVISVNIPV
610 620 630 640 650
DKIGELIGPK GKTINAIQDE TGADISIEED GAVYIGAVDG PSAEAARAQV
660 670 680 690 700
NAIANPTNPE VGESFLGTVV KIATFGAFVS LLPGKDGLLH ISEVRKLAGG
710 720 730 740 750
KRVENVEDVL GVGQKILVEI TKIDDRGKLS LAPVMEEAAD QEGSAAASDG

PEAPAEG
Length:757
Mass (Da):80,257
Last modified:April 8, 2008 - v1
Checksum:i5AD9D620E4A268E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM849034 Genomic DNA. Translation: CAQ01301.1.
RefSeqiWP_012298580.1. NC_010407.1.

Genome annotation databases

EnsemblBacteriaiCAQ01301; CAQ01301; CMS1186.
KEGGicms:CMS1186.
PATRICi21459368. VBIClaMic4666_1246.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM849034 Genomic DNA. Translation: CAQ01301.1.
RefSeqiWP_012298580.1. NC_010407.1.

3D structure databases

ProteinModelPortaliB0RGW7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi31964.CMS_1186.

Proteomic databases

PRIDEiB0RGW7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAQ01301; CAQ01301; CMS1186.
KEGGicms:CMS1186.
PATRICi21459368. VBIClaMic4666_1246.

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNP_CLAMS
AccessioniPrimary (citable) accession number: B0RGW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: April 8, 2008
Last modified: November 2, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.