B0RGI6 (ISPE_CLAMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	CMS2305

Organism

Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / JCM 9667) [Complete proteome] [HAMAP]

Taxonomic identifier

31964 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335707
Regions
	Nucleotide binding	104 – 114	11	ATP Potential
Sites
	Active site	18	1	By similarity
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0RGI6 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 8EDC7EDB478644A4	FASTA	312	32,107
	10 20 30 40 50 60 MTSAATSSDA VHARAPGKIN VSLTVGALQE DGYHDVATAY QAVSLYEDVY ATRSDGFSVE 70 80 90 100 110 120 FGGSIDTSRL TTGGDNLAIR AARLLARSTG HRGGVHLRIE KDVPIAGGMG GGSADAAATL 130 140 150 160 170 180 LACDTLWGTE RTRDQLLALG AELGADVPFA LAGGTAIGTG RGDRLSPALA KGTFQWVLAI 190 200 210 220 230 240 AEFGVSTPDV YGELDKHRER HAQDIFPAQQ IPQVDSGVLQ ALRAGDPHML AEVLHNDLQA 250 260 270 280 290 300 PALHLAPGLG EVLQLGEENG ALAGIVSGSG PTVAFLASDL DSALELQIAL SAARLQVIRA 310 TGPVHGARII TG

References

[1]

"Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation." Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A. J. Bacteriol. 190:2150-2160(2008) [PubMed: 18192393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33113 / JCM 9667.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM849034 Genomic DNA. Translation: CAQ02392.1.
RefSeq	YP_001710973.1. NC_010407.1.
3D structure databases
ProteinModelPortal	B0RGI6.
ModBase	Search...
Protein-protein interaction databases
STRING	B0RGI6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6157922.
GenomeReviews	Gene locus CMS2305 in contig AM849034_GR.
KEGG	cms:CMS_2305.
PATRIC	21461647. VBIClaMic4666_2367.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	KINLHLG.
ProtClustDB	PRK03188.
Enzyme and pathway databases
BioCyc	CMIC31964:CMS2305-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_CLAMS

Accession

Primary (citable) accession number: B0RGI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program