B0REP5 (EFTS_CLAMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor Ts
Short name=EF-Ts

Gene names

Name:	tsf
Ordered Locus Names:	CMS0772

Organism

Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / JCM 9667) [Complete proteome] [HAMAP]

Taxonomic identifier

31964 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome By similarity. HAMAP MF_00050
Subcellular location	Cytoplasm By similarity HAMAP MF_00050.
Sequence similarities	Belongs to the EF-Ts family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Elongation factor Ts HAMAP MF_00050		PRO_1000074857
Regions
	Region	80 – 83	4	Involved in Mg(2+) ion dislocation from EF-Tu By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0REP5 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 8258CC6869C67970	FASTA	275	29,357
	10 20 30 40 50 60 MANFTAADVK ELRDRLGAGM MDSKNALVEA DGDIEKAIEI LRLKGQKGNA KRGDRSTAEG 70 80 90 100 110 120 LVAASEQDGA ATLIELACET DFVAKNDKFI ALSESVLAAV VAAGASTVEE ALQAPAGEQT 130 140 150 160 170 180 VDQLISDRAA ILGEKIALRR VARLAGEHQE IYLHRTSKDL PPQVGVVVDY SGTDAETARS 190 200 210 220 230 240 IAQHIAFANP EYLAREDVPA DKVEAERAIV TEISRNEGKP EAALPKIIEG RLTGFFKQVA 250 260 270 LLEQDYAKDN KQSVKKVVEA AGLTVTGFAR FKVGA

References

[1]

"Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation." Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A. J. Bacteriol. 190:2150-2160(2008) [PubMed: 18192393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33113 / JCM 9667.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM849034 Genomic DNA. Translation: CAQ00892.1.
RefSeq	YP_001709535.1. NC_010407.1.
3D structure databases
ProteinModelPortal	B0REP5.
ModBase	Search...
Protein-protein interaction databases
STRING	B0REP5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6158965.
GenomeReviews	Gene locus CMS0772 in contig AM849034_GR.
KEGG	cms:CMS_0772.
PATRIC	21458546. VBIClaMic4666_0834.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG713289.
OMA	YLHGTRI.
ProtClustDB	PRK09377.
Enzyme and pathway databases
BioCyc	CMIC31964:CMS0772-MONOMER.
Family and domain databases
HAMAP	MF_00050. EF_Ts. [Tree]
InterPro	IPR001816. Transl_elong_EFTs/EF1B. IPR014039. Transl_elong_EFTs/EF1B_dimer. IPR009060. UBA-like. IPR000449. UBA/transl_elong_EF1B_N. [Graphical view]
Gene3D	G3DSA:3.30.479.20. Transl_elong_EFTs/EF1B_dimer. 3 hits.
KO	K02357.
PANTHER	PTHR11741. Transl_elong_EFTs/EF1B. 1 hit.
Pfam	PF00889. EF_TS. 1 hit. PF00627. UBA. 1 hit. [Graphical view]
SUPFAM	SSF54713. EF_TS. 1 hit. SSF46934. UBA_like. 1 hit.
TIGRFAMs	TIGR00116. Tsf. 1 hit.
PROSITE	PS01126. EF_TS_1. False negative. PS01127. EF_TS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTS_CLAMS

Accession

Primary (citable) accession number: B0REP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program