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B0RE24

- LIPA_CLAMS

UniProt

B0RE24 - LIPA_CLAMS

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Protein

Lipoyl synthase

Gene

lipA

Organism
Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi81 – 811Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi85 – 851Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi88 – 881Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCMIC31964:GJBN-1895-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:CMS1883
OrganismiClavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Taxonomic identifieri31964 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter
ProteomesiUP000001318: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Lipoyl synthasePRO_1000204144Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi31964.CMS_1883.

Structurei

3D structure databases

ProteinModelPortaliB0RE24.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B0RE24-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAAPEGRRM LRLEVRNAET PIERKPEWIK TKARMGPEYQ ALQQLVKTED
60 70 80 90 100
LHTVCQEAAC PNIYECWEDR EATFLIGGSQ CTRRCDFCQI DTGKPADYDT
110 120 130 140 150
DEPRRVADSV RRMGLRYATV TGVARDDLPD EGAWLHAETV RRIHADNPGT
160 170 180 190 200
GVEILATDFS GNPDLLAEVF SSRPEVFAHN VETVPRIFKR IRPAFRYERS
210 220 230 240 250
LDVITQGRDA DLITKSNLIL GMGETREEVS EALADLHDAG CDIITVTQYL
260 270 280 290 300
RPSPRHLPVA RWVRPEEFVE IKAEAEAIGF LGVLAGPLVR SSYRAGRLYA
310 320
QSMSAKGREL PASLAHLADP ANGFAQAVG
Length:329
Mass (Da):36,659
Last modified:April 8, 2008 - v1
Checksum:iDCFA7A13ABC652D7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM849034 Genomic DNA. Translation: CAQ01985.1.
RefSeqiWP_012299221.1. NC_010407.1.
YP_001710584.1. NC_010407.1.

Genome annotation databases

EnsemblBacteriaiCAQ01985; CAQ01985; CMS1883.
GeneIDi6158796.
KEGGicms:CMS_1883.
PATRICi21460808. VBIClaMic4666_1950.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM849034 Genomic DNA. Translation: CAQ01985.1 .
RefSeqi WP_012299221.1. NC_010407.1.
YP_001710584.1. NC_010407.1.

3D structure databases

ProteinModelPortali B0RE24.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 31964.CMS_1883.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAQ01985 ; CAQ01985 ; CMS1883 .
GeneIDi 6158796.
KEGGi cms:CMS_1883.
PATRICi 21460808. VBIClaMic4666_1950.

Phylogenomic databases

eggNOGi COG0320.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci CMIC31964:GJBN-1895-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation."
    Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A.
    J. Bacteriol. 190:2150-2160(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1.

Entry informationi

Entry nameiLIPA_CLAMS
AccessioniPrimary (citable) accession number: B0RE24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: April 8, 2008
Last modified: October 29, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3