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Protein

Glutamate 5-kinase

Gene

proB

Organism
Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei18ATPUniRule annotation1
Binding sitei54SubstrateUniRule annotation1
Binding sitei141SubstrateUniRule annotation1
Binding sitei153Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi173 – 174ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:CMS1813
OrganismiClavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Taxonomic identifieri31964 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrobacteriaceaeClavibacter
Proteomesi
  • UP000001318 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000810511 – 259Glutamate 5-kinaseAdd BLAST259

Interactioni

Protein-protein interaction databases

STRINGi31964.CMS_1813

Structurei

3D structure databases

ProteinModelPortaliB0RDG2
SMRiB0RDG2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit

Sequencei

Sequence statusi: Complete.

B0RDG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTASRAGIA SARRIVVKVG SSSISGENAG QIAPLVDAIA AAHARGAEVV
60 70 80 90 100
LVSSGAIATG MPFLRLDDRP ADLATQQAAA AVGQSVLIFR YQESLDRYGI
110 120 130 140 150
VAGQVLLTAG DLAAPDHREN AQRAMERLLG LRLLPVVNEN DTVATHEIRF
160 170 180 190 200
GDNDRLAALV ARLVDADLLL LLSDVDALYS RPPEEPGARR IEHVAFGDEL
210 220 230 240 250
EGVEIGSTGT GVGTGGAVTK VAAARLAAEA GTGVLLTSTA QVAEALAGAH

VGTWFAPRS
Length:259
Mass (Da):26,629
Last modified:April 8, 2008 - v1
Checksum:i3D06C711C41E7F35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM849034 Genomic DNA Translation: CAQ01918.1
RefSeqiWP_012299159.1, NZ_MZMN01000003.1

Genome annotation databases

EnsemblBacteriaiCAQ01918; CAQ01918; CMS1813
GeneIDi29470263
KEGGicms:CMS1813

Similar proteinsi

Entry informationi

Entry nameiPROB_CLAMS
AccessioniPrimary (citable) accession number: B0RDG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: May 23, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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