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B0RDA6 (SYL_CLAMS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CMS1760
OrganismClavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum) [Complete proteome] [HAMAP]
Taxonomic identifier31964 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter

Protein attributes

Sequence length852 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 852852Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334744

Regions

Motif51 – 6111"HIGH" region HAMAP-Rule MF_00049
Motif615 – 6195"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6181ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B0RDA6 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 3FFE4D7CEC276D8D

FASTA85294,838
        10         20         30         40         50         60 
MAHETPDTPG ETYDFRAIEA EWSEVWEREQ PFRTPDASDS RPRKYILDMF PYPSGDLHMG 

        70         80         90        100        110        120 
HAEAFALGDA VARYWRQQGF NVLHPIGWDS FGLPAENAAI KRGVDPREWT YANIETQKQS 

       130        140        150        160        170        180 
MKRYGLSFDW ERELHTSDPE YYRWNQWLFL KMHEKGLAYR KDSWVNWDPV DQTVLANEQV 

       190        200        210        220        230        240 
LPDGTSDRSG AVVVKKKLTQ WYLRITDYAD RLVDDLNQLE GTWPAKVLSM QRNWIGRSIG 

       250        260        270        280        290        300 
AEVDFVVEGR DEPVTVFTTR PDTLHGATFM VVAPDSDLAA ELVEGASEEV RESFRGYLER 

       310        320        330        340        350        360 
TQRLNEIERS TTDRPKTGIP LGLTAINPVN GERIPVWAAD YVLADYGTGA VMAVPAHDQR 

       370        380        390        400        410        420 
DLDFARAFDL PVRVVVDTTQ PVTGAIRIIP EDGELPDLEE VLPGRTGVAL PGEGRLINSG 

       430        440        450        460        470        480 
SLNGLSKQPA IKRVIEQLEA EGRGRAAKNY RLRDWLISRQ RFWGTPIPIV YDAEGSEIRV 

       490        500        510        520        530        540 
PEDQLPVRLP DTEGLDLTPK GKSPLAAATA WSNVPSPVDG SPATRDPDTM DTFMDSSWYW 

       550        560        570        580        590        600 
LRFLSPNDAT KAFDPADADR WAPIDQYVGG VEHAILHLLY SRFITKVLFD LGYVTFTEPF 

       610        620        630        640        650        660 
SALLNQGMVL SGGSKMSKSK GGVDLGSEMD RHGVDAIRLT MAFAGPPEDD IDWEDVSPSG 

       670        680        690        700        710        720 
SAKFLARAWR LTGDITSAPE VEWKTGDEAL RRVTHRFLAE APGMLEAFKF NVVIARTMEL 

       730        740        750        760        770        780 
VNAIRKTIDQ GPGGGDAAVR EATEVVAVAL SLFAPYTAED MWKRLGREGS VAFAGWRKAD 

       790        800        810        820        830        840 
RNLLVQTTVT AVVQVDGKVR DKLEVDAKIG ADELEALARE TAGVRRSTAG RTIDKVIVRA 

       850 
PKIVSITTTP AP 

« Hide

References

[1]"Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation."
Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A.
J. Bacteriol. 190:2150-2160(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM849034 Genomic DNA. Translation: CAQ01862.1.
RefSeqYP_001710466.1. NC_010407.1.

3D structure databases

ProteinModelPortalB0RDA6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING31964.CMS_1760.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAQ01862; CAQ01862; CMS1760.
GeneID6157515.
KEGGcms:CMS_1760.
PATRIC21460542. VBIClaMic4666_1818.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAMAFAGPP.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCMIC31964:GJBN-1770-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CLAMS
AccessionPrimary (citable) accession number: B0RDA6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries