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B0RCZ5

- MEND_CLAMS

UniProt

B0RCZ5 - MEND_CLAMS

Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

    Catalytic activityi

    Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

    Cofactori

    Magnesium or manganese.UniRule annotation
    Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

    Pathwayi

    GO - Molecular functioni

    1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. thiamine pyrophosphate binding Source: InterPro

    GO - Biological processi

    1. menaquinone biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Menaquinone biosynthesis

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciCMIC31964:GJBN-3011-MONOMER.
    UniPathwayiUPA00079; UER00164.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
    Short name:
    SEPHCHC synthaseUniRule annotation
    Alternative name(s):
    Menaquinone biosynthesis protein MenDUniRule annotation
    Gene namesi
    Name:menDUniRule annotation
    Ordered Locus Names:CMS3017
    OrganismiClavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
    Taxonomic identifieri31964 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter
    ProteomesiUP000001318: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5895892-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthasePRO_0000341725Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi31964.CMS_3017.

    Structurei

    3D structure databases

    ProteinModelPortaliB0RCZ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1165.
    KOiK02551.
    OMAiWRSAVCR.
    OrthoDBiEOG6NWBQW.

    Family and domain databases

    Gene3Di3.40.50.970. 2 hits.
    HAMAPiMF_01659. MenD.
    InterProiIPR004433. MenaQ_synth_MenD.
    IPR029061. THDP-binding.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    IPR011766. TPP_enzyme-bd_C.
    [Graphical view]
    PfamiPF02775. TPP_enzyme_C. 1 hit.
    PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004983. MenD. 1 hit.
    SUPFAMiSSF52518. SSF52518. 2 hits.
    TIGRFAMsiTIGR00173. menD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B0RCZ5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAADALPTS SASASASADG PRTGNPSTDR AIAMLLALVR EGVTDVVLCP    50
    GSRSQALALV AAELERVDGV RLHVRIDERA AGFLALGLGV ESGRPAPVIT 100
    TSGTAVANLH PAVLEGWHSG VPMLLLTGDR PAELRGIASN QTTRQPGMFG 150
    DRVACIDVPA PEETDDDLAR DALLARDAYR RARDERTPVH VNVAFRDPLS 200
    VAVPDLTEAV AEVRAAAPAT PAPAGPATAD VLDLPHGPRT LVVAGHAAGE 250
    AAEELARAGG WPLAAEISSG SHFGPNLVVS FRELLAREGF GDRVERVIVF 300
    GHPTLTREVP LLVGREDVEA IVVGSTGGED YDPRHRVTAH PAAVRVVGEP 350
    ADPAEARRWL GTWVHESRAI LDEATAAESA PLLPSGTTPA ERRDFARAEL 400
    AAVRADVTRR HLVRALWQAT WPHDRLVLGA SRLIREADRA LPGKRVRVHA 450
    NRGLAGIDGT ISTGLGIALA SQAGSGSAAA GITRVLVGDL TLLHDVGSLL 500
    IGTGERVPRI QVIVGNDGGG TIFDGLEVSR TAAPASIDRV MFTPQRVDLA 550
    SLARAYGWAH LRAATHGELE AALTTASEAP LLIEVPLAR 589
    Length:589
    Mass (Da):61,555
    Last modified:April 8, 2008 - v1
    Checksum:i82920B3D1DDA1B78
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM849034 Genomic DNA. Translation: CAQ03087.1.
    RefSeqiWP_012300234.1. NC_010407.1.
    YP_001711643.1. NC_010407.1.

    Genome annotation databases

    EnsemblBacteriaiCAQ03087; CAQ03087; CMS3017.
    GeneIDi6158519.
    KEGGicms:CMS_3017.
    PATRICi21463057. VBIClaMic4666_3062.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM849034 Genomic DNA. Translation: CAQ03087.1 .
    RefSeqi WP_012300234.1. NC_010407.1.
    YP_001711643.1. NC_010407.1.

    3D structure databases

    ProteinModelPortali B0RCZ5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 31964.CMS_3017.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAQ03087 ; CAQ03087 ; CMS3017 .
    GeneIDi 6158519.
    KEGGi cms:CMS_3017.
    PATRICi 21463057. VBIClaMic4666_3062.

    Phylogenomic databases

    eggNOGi COG1165.
    KOi K02551.
    OMAi WRSAVCR.
    OrthoDBi EOG6NWBQW.

    Enzyme and pathway databases

    UniPathwayi UPA00079 ; UER00164 .
    BioCyci CMIC31964:GJBN-3011-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.970. 2 hits.
    HAMAPi MF_01659. MenD.
    InterProi IPR004433. MenaQ_synth_MenD.
    IPR029061. THDP-binding.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    IPR011766. TPP_enzyme-bd_C.
    [Graphical view ]
    Pfami PF02775. TPP_enzyme_C. 1 hit.
    PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004983. MenD. 1 hit.
    SUPFAMi SSF52518. SSF52518. 2 hits.
    TIGRFAMsi TIGR00173. menD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation."
      Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A.
      J. Bacteriol. 190:2150-2160(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1.

    Entry informationi

    Entry nameiMEND_CLAMS
    AccessioniPrimary (citable) accession number: B0RCZ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 2008
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3