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B0RCZ5

- MEND_CLAMS

UniProt

B0RCZ5 - MEND_CLAMS

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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Magnesium or manganese.UniRule annotation
Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Menaquinone biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciCMIC31964:GJBN-3011-MONOMER.
UniPathwayiUPA00079.
UPA01057; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:CMS3017
OrganismiClavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)
Taxonomic identifieri31964 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter
ProteomesiUP000001318: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5895892-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthasePRO_0000341725Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi31964.CMS_3017.

Structurei

3D structure databases

ProteinModelPortaliB0RCZ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1165.
KOiK02551.
OMAiWRSAVCR.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

B0RCZ5 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAADALPTS SASASASADG PRTGNPSTDR AIAMLLALVR EGVTDVVLCP
60 70 80 90 100
GSRSQALALV AAELERVDGV RLHVRIDERA AGFLALGLGV ESGRPAPVIT
110 120 130 140 150
TSGTAVANLH PAVLEGWHSG VPMLLLTGDR PAELRGIASN QTTRQPGMFG
160 170 180 190 200
DRVACIDVPA PEETDDDLAR DALLARDAYR RARDERTPVH VNVAFRDPLS
210 220 230 240 250
VAVPDLTEAV AEVRAAAPAT PAPAGPATAD VLDLPHGPRT LVVAGHAAGE
260 270 280 290 300
AAEELARAGG WPLAAEISSG SHFGPNLVVS FRELLAREGF GDRVERVIVF
310 320 330 340 350
GHPTLTREVP LLVGREDVEA IVVGSTGGED YDPRHRVTAH PAAVRVVGEP
360 370 380 390 400
ADPAEARRWL GTWVHESRAI LDEATAAESA PLLPSGTTPA ERRDFARAEL
410 420 430 440 450
AAVRADVTRR HLVRALWQAT WPHDRLVLGA SRLIREADRA LPGKRVRVHA
460 470 480 490 500
NRGLAGIDGT ISTGLGIALA SQAGSGSAAA GITRVLVGDL TLLHDVGSLL
510 520 530 540 550
IGTGERVPRI QVIVGNDGGG TIFDGLEVSR TAAPASIDRV MFTPQRVDLA
560 570 580
SLARAYGWAH LRAATHGELE AALTTASEAP LLIEVPLAR
Length:589
Mass (Da):61,555
Last modified:April 8, 2008 - v1
Checksum:i82920B3D1DDA1B78
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM849034 Genomic DNA. Translation: CAQ03087.1.
RefSeqiWP_012300234.1. NC_010407.1.
YP_001711643.1. NC_010407.1.

Genome annotation databases

EnsemblBacteriaiCAQ03087; CAQ03087; CMS3017.
GeneIDi6158519.
KEGGicms:CMS_3017.
PATRICi21463057. VBIClaMic4666_3062.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM849034 Genomic DNA. Translation: CAQ03087.1 .
RefSeqi WP_012300234.1. NC_010407.1.
YP_001711643.1. NC_010407.1.

3D structure databases

ProteinModelPortali B0RCZ5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 31964.CMS_3017.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAQ03087 ; CAQ03087 ; CMS3017 .
GeneIDi 6158519.
KEGGi cms:CMS_3017.
PATRICi 21463057. VBIClaMic4666_3062.

Phylogenomic databases

eggNOGi COG1165.
KOi K02551.
OMAi WRSAVCR.
OrthoDBi EOG6NWBQW.

Enzyme and pathway databases

UniPathwayi UPA00079 .
UPA01057 ; UER00164 .
BioCyci CMIC31964:GJBN-3011-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
HAMAPi MF_01659. MenD.
InterProi IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view ]
Pfami PF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004983. MenD. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00173. menD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation."
    Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A.
    J. Bacteriol. 190:2150-2160(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1.

Entry informationi

Entry nameiMEND_CLAMS
AccessioniPrimary (citable) accession number: B0RCZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: April 8, 2008
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3