B0RC22 (B0RC22_CLAMS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116
Short name=dUTPase HAMAP MF_00116
EC=3.6.1.23 HAMAP MF_00116

Alternative name(s):
dUTP pyrophosphatase HAMAP MF_00116

Gene names

Name:	dut HAMAP MF_00116
Ordered Locus Names:	CMS1640

Organism

Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / JCM 9667) [Complete proteome] [HAMAP] EMBL CAQ01750.1

Taxonomic identifier

31964 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity. HAMAP MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family. HAMAP MF_00116

Ontologies

Keywords
Biological process	Nucleotide metabolism HAMAP MF_00116
Ligand	Magnesium HAMAP MF_00116 Metal-binding HAMAP MF_00116
Molecular function	Hydrolase HAMAP MF_00116 EMBL CAQ01750.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	63 – 65	3	Substrate binding By similarity HAMAP MF_00116
	Region	80 – 82	3	Substrate binding By similarity HAMAP MF_00116
Sites
	Binding site	76	1	Substrate By similarity HAMAP MF_00116

Sequences

	Sequence		Length	Mass (Da)
	B0RC22 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: C9A9FCCDC972FBCF	FASTA	156	15,877
	10 20 30 40 50 60 MPDPVDVLIA GDAPIPTYAH PGDAGADLTA AEAVRLEPGR RATVGTGVSI ALPDGYAAFV 70 80 90 100 110 120 LPRSGLAARH GITIVNAPGT VDAGYRGEIR VTLLNTDAEA AYDVAVGDRI AQVVVMPVSR 130 140 150 VRFVPVEKLP GSHRGTGGFG STGTGALPAR DPGDRT

References

[1]

"Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation." Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A. J. Bacteriol. 190:2150-2160(2008) [PubMed: 18192393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM849034 Genomic DNA. Translation: CAQ01750.1.
RefSeq	YP_001710363.1. NC_010407.1.
3D structure databases
ProteinModelPortal	B0RC22.
SMR	B0RC22. Positions 8-130.
ModBase	Search...
Protein-protein interaction databases
STRING	B0RC22.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6157422.
GenomeReviews	Gene locus CMS1640 in contig AM849034_GR.
KEGG	cms:CMS_1640.
PATRIC	21460314. VBIClaMic4666_1705.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG436079.
OMA	KIAQMVI.
ProtClustDB	CLSK831200.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B0RC22_CLAMS

Accession

Primary (citable) accession number: B0RC22

Entry history

Integrated into UniProtKB/TrEMBL:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)