B0RBL3 (GSA_CLAMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase
Short name=GSA
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name=GSA-AT

Gene names

Name:	hemL
Ordered Locus Names:	CMS2838

Organism

Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / JCM 9667) [Complete proteome] [HAMAP]

Taxonomic identifier

31964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter › Clavibacter michiganensis

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate. HAMAP-Rule MF_00375
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. HAMAP-Rule MF_00375
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00375.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	Glutamate-1-semialdehyde 2,1-aminomutase HAMAP-Rule MF_00375		PRO_1000079916
Amino acid modifications
	Modified residue	286	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0RBL3 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: FABFDF4DF5B275C0	FASTA	455	47,238
	10 20 30 40 50 60 MTHSQDLFDR ARDVIPGGVN SPVRAFGSVG GTPRMMVRAA GPYVTDADGV EYVDLVNSWG 70 80 90 100 110 120 PAILGHARPE VVKAVQDAAA LGLGFGATTP AETELAELVT ERVRVAGVDG SPDRRPVEKL 130 140 150 160 170 180 RLVSTGTEAT MTAIRLARGF TGRDLLVKFA GHYHGHSDSL LAEAGSGVAT LALPGSAGIP 190 200 210 220 230 240 EAIAAQTIVV PYNDLGAVRA VFAEHGPRIA AVITEAAAAN MGVVPPLPGF TAELARIAHD 250 260 270 280 290 300 NGSLLISDEV LTGFRVHPAG YWGLDNDGLA ADHPDAWTPD LVTYGKVIGG GLPVAALGGR 310 320 330 340 350 360 ADVMDHLAPL GPVYQAGTLS GNPVAVAAGL TTLRLADADV YRALDIAADI LIYAVELAFD 370 380 390 400 410 420 RAGLAYSVQR AGSLFSFTFG TPPEHGITDY ATVQAQETWR YPAFFHSMLD QGVSLPPSVF 430 440 450 EAWFVSAAMD EASLDRVIRA LPAAARAAAA ATPPA

References

[1]

"Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation." Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A. J. Bacteriol. 190:2150-2160(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33113 / JCM 9667.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM849034 Genomic DNA. Translation: CAQ02909.1.
RefSeq	YP_001711471.1. NC_010407.1.
3D structure databases
ProteinModelPortal	B0RBL3.
ModBase	Search...
Protein-protein interaction databases
STRING	31964.CMS_2838.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAQ02909; CAQ02909; CMS2838.
GeneID	6158369.
KEGG	cms:CMS_2838.
PATRIC	21462713. VBIClaMic4666_2891.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0001.
KO	K01845.
OMA	FNGNPIS.
ProtClustDB	PRK00062.
Enzyme and pathway databases
BioCyc	CMIC31964:GJBN-2831-MONOMER.
UniPathway	UPA00251; UER00317.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
HAMAP	MF_00375. HemL_aminotrans_3.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_CLAMS

Accession

Primary (citable) accession number: B0RBL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program