B0RB95 (B0RB95_CLAMS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Ordered Locus Names:

CMS1525

Organism

Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / JCM 9667) [Complete proteome] [HAMAP] EMBL CAQ01636.1

Taxonomic identifier

31964 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	252	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	289	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	385	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	B0RB95 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: CE9CE032B7CE3BCF	FASTA	600	65,723
	10 20 30 40 50 60 MTDDRFDIWA PKARTLALSV GDERLPLSPV GDGWWTLDAA RAEALPSGDL DYGYLVDDSG 70 80 90 100 110 120 TPLPDPRSRR QPEGVHGRSR TYDPSSFAWT DQAWTGRQLA GAVVYEMHIG TFTPEGTLDS 130 140 150 160 170 180 AIDRLDHLVA LGVDLVEVLP VNGFNGTHNW GYDGVLWYAV QETYGGPEAY QRFVDACHAR 190 200 210 220 230 240 GLGVVQDVVY NHLGPSGNYL PVYGPYLHEA SANTWGSSLN LDGEDSGPVR EYIIDNALMW 250 260 270 280 290 300 LGDYHVDALR LDAVHALVDD TATHLLEELA VQVDVLSAHV GRPLTLIAES DLNDPKLITS 310 320 330 340 350 360 REAHGYGLDA QWSDDFHHAV HVALTGETTG YYADFAPLGA IAKVITRGFF HDGTWSSFRG 370 380 390 400 410 420 RVHGRPLDTE RIPAHRLVVA NQNHDQIGNR ATGDRLTATL DEGGLALAAV LTLTSPFTPM 430 440 450 460 470 480 LFMGEEWGAT TPWQFFTSHP EHDLGEATAK GRIAEFAKMG WDESVVPNPQ DLSTFQDSKL 490 500 510 520 530 540 DWSELYGAEA ADSQHARLFA LYSELIRLRR THPDLTDPRF AEVEVEVHEE ARLLVMDRGE 550 560 570 580 590 600 LSIVVNLSDE ERRVPVVGDR PAMLFATEPG VSLGADEVVL PARSAAILGP VADSAEALLA

References

[1]

"Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation." Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A. J. Bacteriol. 190:2150-2160(2008) [PubMed: 18192393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM849034 Genomic DNA. Translation: CAQ01636.1.
RefSeq	YP_001710251.1. NC_010407.1.
3D structure databases
ProteinModelPortal	B0RB95.
ModBase	Search...
Protein-protein interaction databases
STRING	B0RB95.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6157339.
GenomeReviews	Gene locus CMS1525 in contig AM849034_GR.
KEGG	cms:CMS_1525.
PATRIC	21460072. VBIClaMic4666_1588.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG367595.
OMA	EGFVYQG.
ProtClustDB	CLSK2320911.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR022567. Maltooligo_trehalose_bac_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. PF11941. DUF3459. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B0RB95_CLAMS

Accession

Primary (citable) accession number: B0RB95

Entry history

Integrated into UniProtKB/TrEMBL:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	December 14, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)