B0RB42 (RL2_CLAMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L2

Gene names

Name:	rplB
Ordered Locus Names:	CMS0286

Organism

Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / JCM 9667) [Complete proteome] [HAMAP]

Taxonomic identifier

31964 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome By similarity. HAMAP MF_01320_B
Subunit structure	Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome By similarity.
Sequence similarities	Belongs to the ribosomal protein L2P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	50S ribosomal protein L2 HAMAP MF_01320_B		PRO_1000086324

Sequences

	Sequence		Length	Mass (Da)
	B0RB42 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: D27C2030410A4C3E	FASTA	279	30,518
	10 20 30 40 50 60 MAIRKYKPTT PGRRGSSVAD FAEITRSTPE KSLLRPLPKH GGRNNAGRIT TRHIGGGHKR 70 80 90 100 110 120 QYRVIDFKRN DKDGVLATVA HIEYDPNRTA RIALLHFIDG TKRYIIAPNK LKQGDKIESG 130 140 150 160 170 180 AQADIKPGNN LPLRNIPTGT VIHAIELRPG GGAKMGRSAG ASVRLVAKDG PYAQLRLPSG 190 200 210 220 230 240 EIRNVDARCR ATIGEVGNAE QSNINWGKAG RMRWKGVRPT VRGVAMNPVD HPHGGGEGKT 250 260 270 SGGRHPVSPW GQKEGRTRHI NKPSDKLIVR RRNAGKKRK

References

[1]

"Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation." Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A. J. Bacteriol. 190:2150-2160(2008) [PubMed: 18192393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33113 / JCM 9667.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM849034 Genomic DNA. Translation: CAQ00407.1.
RefSeq	YP_001709068.1. NC_010407.1.
3D structure databases
ProteinModelPortal	B0RB42.
SMR	B0RB42. Positions 2-271.
ModBase	Search...
Protein-protein interaction databases
STRING	B0RB42.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6158952.
GenomeReviews	Gene locus CMS0286 in contig AM849034_GR.
KEGG	cms:CMS_0286.
PATRIC	21457544. VBIClaMic4666_0345.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG748739.
OMA	QSNINWG.
ProtClustDB	PRK09374.
Enzyme and pathway databases
BioCyc	CMIC31964:CMS0286-MONOMER.
Family and domain databases
HAMAP	MF_01320_B. Ribosomal_L2_B. [Tree]
InterPro	IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR022666. Rbsml_prot_L2_RNA-bd_dom. IPR002171. Ribosomal_L2. IPR014726. Ribosomal_L2_3. IPR005880. Ribosomal_L2_bac-type. IPR022669. Ribosomal_L2_C. IPR022671. Ribosomal_L2_CS. IPR014722. Transl_SH3-like_sub. IPR008991. Translation_prot_SH3-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:2.30.30.30. Ribosomal_L2. 1 hit. G3DSA:4.10.950.10. Ribosomal_L2. 1 hit.
KO	K02886.
PANTHER	PTHR13691:SF5. Ribosom_L2_bac. 1 hit. PTHR13691. Ribosomal_L2. 1 hit.
Pfam	PF00181. Ribosomal_L2. 1 hit. PF03947. Ribosomal_L2_C. 1 hit. [Graphical view]
PIRSF	PIRSF002158. Ribosomal_L2. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF50104. Transl_SH3_like. 1 hit.
TIGRFAMs	TIGR01171. RplB_bact. 1 hit.
PROSITE	PS00467. RIBOSOMAL_L2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RL2_CLAMS

Accession

Primary (citable) accession number: B0RB42

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program