B0RAP7 (SYR_CLAMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	CMS1474

Organism

Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / JCM 9667) [Complete proteome] [HAMAP]

Taxonomic identifier

31964 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Clavibacter

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity. HAMAP MF_00123
Subcellular location	Cytoplasm By similarity HAMAP MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Arginine--tRNA ligase HAMAP MF_00123		PRO_1000076208
Regions
	Motif	132 – 142	11	"HIGH" region HAMAP MF_00123

Sequences

	Sequence		Length	Mass (Da)
	B0RAP7 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: C6272609C2F4A7BA	FASTA	554	60,487
	10 20 30 40 50 60 MTTPDLTGAL FEIVARTAGR RPGGDAIALS PDMVVLERPR NRDHGDWATN IAMRIAKPLG 70 80 90 100 110 120 ESPRTIAADI AKALGELPQV AKVDVAGPGF INITLEAAAA GALAHTIVES GPAYGRGHSL 130 140 150 160 170 180 EGIRINLEFV SANPTGPIHL GGVRWAAVGD SLARILQAEG ADVTREYYFN DHGSQIDRFA 190 200 210 220 230 240 RSLLASHLGE ETPEDGYGGA YIGEIAERVV EGYEGDIDAL TREAQQEVFR KSGTELMFGE 250 260 270 280 290 300 IKQKLHDFGV DFDVFFHEDS LHESGAVDRA IARLTELGHV FEEDGAIWLR TTTFGDDRDR 310 320 330 340 350 360 VVIRSTGEPA YISGDLGYYL DKRERGFEQN IIMLGADHHG YVGRMMAMVE AFGDTPGVNL 370 380 390 400 410 420 QILIGQMVNL LRDGEPVRMS KRAGTIVTLD DLVDAVGVDA GRYALVRSSA DQNLDIDLAV 430 440 450 460 470 480 LGKRTNDNPV FYVQYAHART CAVDRNAAAS GVDRSAFAPE LLTHPTESAL LGLLQEFPRI 490 500 510 520 530 540 VAQAAELREP HRVARYVEEL AGSYHRWYDS CRVVPRGDEE VTDLHRTRLW LNDAVRQVVA 550 NGLNLVGVSA PERM

References

[1]

"Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation." Bentley S.D., Corton C., Brown S.E., Barron A., Clark L., Doggett J., Harris B., Ormond D., Quail M.A., May G., Francis D., Knudson D., Parkhill J., Ishimaru C.A. J. Bacteriol. 190:2150-2160(2008) [PubMed: 18192393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33113 / JCM 9667.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM849034 Genomic DNA. Translation: CAQ01585.1.
RefSeq	YP_001710201.1. NC_010407.1.
3D structure databases
ProteinModelPortal	B0RAP7.
ModBase	Search...
Protein-protein interaction databases
STRING	B0RAP7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6157300.
GenomeReviews	Gene locus CMS1474 in contig AM849034_GR.
KEGG	cms:CMS_1474.
PATRIC	21459964. VBIClaMic4666_1536.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG695395.
OMA	RMKAAMK.
ProtClustDB	PRK01611.
Enzyme and pathway databases
BioCyc	CMIC31964:CMS1474-MONOMER.
Family and domain databases
HAMAP	MF_00123. Arg_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01887.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. ArgS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_CLAMS

Accession

Primary (citable) accession number: B0RAP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program