B0R9V8 (KATG_HALS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	OE5186R

Encoded on

Plasmid PHS3

Organism

Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) [Complete proteome] [HAMAP]

Taxonomic identifier

478009 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 720	720	Catalase-peroxidase HAMAP MF_01961		PRO_0000354966
Sites
	Active site	83	1	Proton acceptor By similarity
	Metal binding	248	1	Iron (heme axial ligand) By similarity
	Site	79	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	82 ↔ 207		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-233) By similarity
	Cross-link	207 ↔ 233		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-82) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0R9V8 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: FB88823BCD3CB2F9	FASTA	720	80,477
	10 20 30 40 50 60 MENEDHNFGT SDWWPNQLDL EILDQNSQQV DPYGEDFDYA EAFEDLDLAA VKDDLEEMMT 70 80 90 100 110 120 DSKDWWPADY GHYGPLFIRM AWHSAGTYRT FDGRGGAAGG RQRLPPVDSW PDNVNLDKAR 130 140 150 160 170 180 RLLWPIKQKY GRKLSWGDLI ILAGNVALES MGFETYGFAG GRKDDYTPDE AVDWGPEDEW 190 200 210 220 230 240 ETTSGDRFDA DGSLKWPLGN TVMGLIYVNP EGPNGEPDLE GSAKNIRESF GKMAMNDKET 250 260 270 280 290 300 VALIAGGHTF GKVHGADDPE ENVGAEPAAA PIEKQGLGWE NEFGEGKGPD TITSGIEGPW 310 320 330 340 350 360 NTTPTQWDMS YVDNLLEYEW EPEKGPGGAW QWTTKSGELN ESAPGVQDPT DTEDVMMLTT 370 380 390 400 410 420 DVALKDDPDY REVLETFQEN PREFQQSFSK AWYKLIHRDM GPSERFLGPE VPEETMIWQD 430 440 450 460 470 480 PLPDADYDLV DDEAVAALKS ELLESELSIP QLVKTAWASA STYRDSDKRG GANGARIRLE 490 500 510 520 530 540 PQRSWEVNEP EQLEAALSTY EDIQAEFNDA RSDDMRVSLA DLIVLGGNAA IEQAAADAGY 550 560 570 580 590 600 DVDVPFEPGR TDATPEQTDV ESFEALKPKA DGFRNYLGDD AEREPEELLV DKAELLNLTA 610 620 630 640 650 660 DDMTVLVGGL RALGVTHGDS ELGIFTDQPG TLTNDFFTTL LDMDYEWEAA SEDREVFELR 670 680 690 700 710 720 DRETGDVEWT GSRVDLLFGS NTRLRAIAEV YGSDADEELF VQDFVDTWSE VMKLDRFDLE

References

[1]

"Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346(2008) [PubMed: 18313895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29341 / DSM 671 / R1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM774418 Genomic DNA. Translation: CAP15539.1.
RefSeq	YP_001690672.1. NC_010368.1.
3D structure databases
ProteinModelPortal	B0R9V8.
SMR	B0R9V8. Positions 12-720.
ModBase	Search...
Protein-protein interaction databases
STRING	B0R9V8.
Proteomic databases
PRIDE	B0R9V8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5954764.
GenomeReviews	Gene locus OE5186R in contig AM774418_GR.
KEGG	hsl:OE5186R.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KRHAPSM.
PhylomeDB	B0R9V8.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	HSAL478009:OE5186R-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_HALS3

Accession

Primary (citable) accession number: B0R9V8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	April 8, 2008
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program