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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42NADUniRule annotation1
Binding sitei69NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi167Iron; shared with adjacent protomerUniRule annotation1
Binding sitei167NAD; shared with adjacent protomerUniRule annotation1
Metal bindingi182IronUniRule annotation1
Binding sitei257NAD; via amide nitrogenUniRule annotation1
Binding sitei267GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi61 – 62NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:OE_4651F
OrganismiHalobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Taxonomic identifieri478009 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
Proteomesi
  • UP000001321 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001156091 – 310Thiamine thiazole synthaseAdd BLAST310

Proteomic databases

PRIDEiB0R884.

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB0R884.
SMRiB0R884.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000106048.
KOiK03146.
OMAiMTEFTDI.
OrthoDBiPOG093Z09PU.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.

Sequencei

Sequence statusi: Complete.

B0R884-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFDSFADAN EAEVTRAITR QWTDEFLDDT ETDVIIVGGG PSGLMAAKEL
60 70 80 90 100
ADRDVDVTII EKNNYLGGGF WLGGFLMNKL TVRSPAEAVL DDLGVPYEYD
110 120 130 140 150
EENDGLAVAD APHACSAMIT AACDAGARIQ NMTEFTDIVV RDDHAVAGAV
160 170 180 190 200
VNWTPVHSLP RELTCVDPIA LEADVVVDAT GHDAVVVSKL HERGVLEADG
210 220 230 240 250
IEHVEEHATG MDQSGDGEYG APGHDSPGHD SMWVADSEDK VVEQTGKVHD
260 270 280 290 300
GLVTAGLSTA TVHGLTRMGP TFGAMLLSGK VAANAVMDEL AVDEPRVDLP
310
STAAPAADDD
Length:310
Mass (Da):32,681
Last modified:April 8, 2008 - v1
Checksum:iB98DBCF375F10E68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM774415 Genomic DNA. Translation: CAP14953.1.
RefSeqiWP_010903946.1. NC_010364.1.

Genome annotation databases

EnsemblBacteriaiCAP14953; CAP14953; OE_4651F.
GeneIDi5953457.
KEGGihsl:OE_4651F.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTHI4_HALS3
AccessioniPrimary (citable) accession number: B0R884
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 8, 2008
Last modified: June 7, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families