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Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.UniRule annotation

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86NucleophileUniRule annotation1
Binding sitei113L-glutamineUniRule annotation1
Active sitei177Charge relay systemUniRule annotation1
Active sitei179Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00245.

Protein family/group databases

MEROPSiC26.A32.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxTUniRule annotation (EC:4.3.3.6UniRule annotation)
Alternative name(s):
Pdx2UniRule annotation
Pyridoxal 5'-phosphate synthase glutaminase subunitUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:pdxTUniRule annotation
Ordered Locus Names:OE_4644R
OrganismiHalobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Taxonomic identifieri478009 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
Proteomesi
  • UP000001321 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003355741 – 203Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST203

Proteomic databases

PRIDEiB0R879.

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB0R879.
SMRiB0R879.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 56L-glutamine bindingUniRule annotation3
Regioni141 – 142L-glutamine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000039949.
KOiK08681.
OMAiVFIRAPI.
OrthoDBiPOG093Z0AY8.

Family and domain databases

CDDicd01749. GATase1_PB. 1 hit.
Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01615. PdxT. 1 hit.
InterProiView protein in InterPro
IPR029062. Class_I_gatase-like.
IPR002161. PdxT/SNO.
IPR021196. PdxT/SNO_CS.
PfamiView protein in Pfam
PF01174. SNO. 1 hit.
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiView protein in PROSITE
PS01236. PDXT_SNO_1. 1 hit.
PS51130. PDXT_SNO_2. 1 hit.

Sequencei

Sequence statusi: Complete.

B0R879-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLTAGVVAV QGDVSEHAAA IRRAADAHGQ PADVREIRTA GVVPECDVLL
60 70 80 90 100
LPGGESTAIS RLLDREGIDA EIRSHVAAGK PLLATCAGLI VSSTDANDDR
110 120 130 140 150
VETLDVLDVT VDRNAFGRQV DSFEAPLDVD GLADPFPAVF IRAPVIDEVG
160 170 180 190 200
ADATVLASWD GRPVAIRDGP VVATSFHPEL TADVRLHELA FFDRTPSAQA

GDA
Length:203
Mass (Da):21,238
Last modified:April 8, 2008 - v1
Checksum:iD96230FF7360A8CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM774415 Genomic DNA. Translation: CAP14948.1.
RefSeqiWP_010903941.1. NC_010364.1.

Genome annotation databases

EnsemblBacteriaiCAP14948; CAP14948; OE_4644R.
GeneIDi5953456.
KEGGihsl:OE_4644R.

Similar proteinsi

Entry informationi

Entry nameiPDXT_HALS3
AccessioniPrimary (citable) accession number: B0R879
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: June 7, 2017
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families