B0R7I0 (SYA_HALS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	OE4198F

Organism

Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) [Complete proteome] [HAMAP]

Taxonomic identifier

478009 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	929 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 929	929	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_0000347879
Sites
	Metal binding	619	1	Zinc By similarity
	Metal binding	623	1	Zinc By similarity
	Metal binding	722	1	Zinc By similarity
	Metal binding	726	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0R7I0 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: E373396311005812	FASTA	929	100,773
	10 20 30 40 50 60 MSDLESAYRL EYFEEEGFHR RECVSCGAHF WTRDGDRETC GEPPCEDYQF IDNPGFDASY 70 80 90 100 110 120 SLTEMRSAMV SYFERAGHDS IDPYPVAANR WRDDVLLTQA SIYDFQPHVT SGASPPPANP 130 140 150 160 170 180 LVVSQPCIRM QDIDNVGKTG RHTMAFEMLG HHAFNADEGT DYAYDGEVYW KDTAVEHCEG 190 200 210 220 230 240 LLADVGVSLD EVTFIEDPWV GGGNAGAAFE VIYRGLELAT LVFMSLERDP DGEYEMKDGH 250 260 270 280 290 300 TYAEMDRRVV DTGYGVERWT WMSQGTPTVY EAVYPDTIDF LKEQAGIEHT DEEAELVHRA 310 320 330 340 350 360 SKHAGNLDID EVADIEDARA GIAAELGVDA ERLTELVAPL EDIYAIADHS RVLAYMFGDG 370 380 390 400 410 420 IVPSNVGTGY LARMVLRRTK RLVDDIGADV PLDELVDMQA DRLGYENRDT IRSIVRSEVE 430 440 450 460 470 480 KYGETLERGG RRVEQLAEEY ADRGEPVPTD ELIELYDSHG IQPGMVADIA ADVGATVDAP 490 500 510 520 530 540 DDFYSLVAAR HDDGDSGAAG GDGGGDDRLA DLPETETLYY DDAYGSEFEA VVLDVFEREG 550 560 570 580 590 600 EDGDGAFDVV LDQTMFYPEG GGQPADTGVL TGDDHTVDVI DVQERDGVVL HRTTDNPGKG 610 620 630 640 650 660 EFVRGQIDTE RRRRLMAHHT ATHIVVYAAR QVLGEHVRQA GAQKGVDSSR IDVTHYERVD 670 680 690 700 710 720 RETVKEIERV ANEIVRANTS VQCEWPDRHE AEAEYGFDLY QGGIPAGEQI RLVHVDDDVQ 730 740 750 760 770 780 ACGGTHVART GEIGSIKILN AERVQDGVER LTFAAGAAAV EHVQGQEDDL RAAAEVLDVT 790 800 810 820 830 840 PAEVPETAER FFTEWKDRGK TIDDLKEQLA EARASGGGAG EEVDVAGTTA VVQRVDGDMD 850 860 870 880 890 900 ELQATANALV DGGQVAVVGS GADGAQFVVG VPDGVPVNAG EVVGELAAMV GGGGGGPPDF 910 920 AQGGGPDAER LDDALSRAAD VLGDAASAE

References

[1]

"Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346(2008) [PubMed: 18313895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29341 / DSM 671 / R1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM774415 Genomic DNA. Translation: CAP14699.1.
RefSeq	YP_001690045.1. NC_010364.1.
3D structure databases
ProteinModelPortal	B0R7I0.
ModBase	Search...
Protein-protein interaction databases
STRING	B0R7I0.
Proteomic databases
PRIDE	B0R7I0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5953322.
GenomeReviews	Gene locus OE4198F in contig AM774415_GR.
KEGG	hsl:OE4198F.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG392147.
OMA	MFTNSGM.
PhylomeDB	B0R7I0.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	HSAL478009:OE4198F-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_HALS3

Accession

Primary (citable) accession number: B0R7I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program