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B0R7F2 (B0R7F2_HALS3) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Adenosylhomocysteinase HAMAP MF_00563 RuleBase RU000548
EC=3.3.1.1 HAMAP MF_00563 RuleBase RU000548
Alternative name(s):
S-adenosyl-L-homocysteine hydrolase HAMAP MF_00563
Gene names
Name:achY EMBL CAP14671.1
Synonyms:ahcY HAMAP MF_00563
Ordered Locus Names:OE4159F
OrganismHalobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) [Complete proteome] [HAMAP]
Taxonomic identifier478009 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine By similarity. HAMAP MF_00563

Catalytic activity

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563 RuleBase RU000548

Cofactor

Binds 1 NAD per subunit By similarity. HAMAP MF_00563

NAD By similarity. RuleBase RU000548

Pathway

Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563

Amino-acid biosynthesis; homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. RuleBase RU000548

Subcellular location

Cytoplasm By similarity HAMAP MF_00563.

Sequence similarities

Belongs to the adenosylhomocysteinase family. RuleBase RU004166 HAMAP MF_00563

Ontologies

Keywords
   Biological processOne-carbon metabolism HAMAP MF_00563 RuleBase RU000548
   Cellular componentCytoplasm HAMAP MF_00563
   LigandNAD HAMAP MF_00563 RuleBase RU000548
   Molecular functionHydrolase HAMAP MF_00563 RuleBase RU000548 EMBL CAP14671.1
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processone-carbon metabolic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionadenosylhomocysteinase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding158 – 1603NAD By similarity HAMAP MF_00563
Nucleotide binding221 – 2266NAD By similarity HAMAP MF_00563
Nucleotide binding300 – 3023NAD By similarity HAMAP MF_00563

Sites

Binding site1321Substrate By similarity HAMAP MF_00563
Binding site1571Substrate By similarity HAMAP MF_00563
Binding site1871Substrate By similarity HAMAP MF_00563
Binding site1911Substrate By similarity HAMAP MF_00563
Binding site1921NAD By similarity HAMAP MF_00563
Binding site2441NAD By similarity HAMAP MF_00563
Binding site2791NAD By similarity HAMAP MF_00563
Binding site3471NAD By similarity HAMAP MF_00563

Sequences

Sequence LengthMass (Da)Tools
B0R7F2 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 6131D032241E1377

FASTA42745,907
        10         20         30         40         50         60 
MTTAPAISSR LDDPESARET GRAKIDWAFE HMPILSALRE EFDANQPLAG ETIGMAMHVE 

        70         80         90        100        110        120 
AKTAALVETM ADAGAEIAIT GCNPLSTHDG VSAALDAHES ITSYAERGAE GEAYYDAIDA 

       130        140        150        160        170        180 
VLAHEPTVTV DDGGDLVFRV HEDHPELIDT IIGGTEETTT GVHRLRAMDD DDALEYPVFA 

       190        200        210        220        230        240 
VNDTPMKRLF DNVHGTGESA LANIAMTTNL SWAGKDVVVA GYGDCGRGVA KKAAGQNANV 

       250        260        270        280        290        300 
IVTEVEPRRA LEAHMEGYDV MPMAEAAEVG DVFLTTTGNK NVITRAHFER MDDGVVLANA 

       310        320        330        340        350        360 
GHFDVEVNLD HLSELAVSER EAREGVREYE LADGRRLNVL AEGRLVNLAS PIGLGHPVGV 

       370        380        390        400        410        420 
MDQSFGVQAV CVRELVANRE EYAAGVHNVP DELDIEIAEI KLAAEGVEYD ALTDDQAEYM 


DSWQHGT

« Hide

References

[1]"Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1."
Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D.
Genomics 91:335-346(2008) [PubMed: 18313895] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29341 / DSM 671 / R1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM774415 Genomic DNA. Translation: CAP14671.1.
RefSeqYP_001690017.1. NC_010364.1.

3D structure databases

ProteinModelPortalB0R7F2.
ModBaseSearch...

Protein-protein interaction databases

STRINGB0R7F2.

Proteomic databases

PRIDEB0R7F2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5954446.
GenomeReviewsGene locus OE4159F in contig AM774415_GR.
KEGGhsl:OE4159F.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG352029.
OMAMEVYAWR.
PhylomeDBB0R7F2.
ProtClustDBPRK05476.

Family and domain databases

HAMAPMF_00563. AdoHcyase.
[Tree]
InterProIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view]
KOK01251.
PANTHERPTHR23420. Ad_hcy_hydrolase. 1 hit.
PfamPF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
PIRSFPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
TIGRFAMsTIGR00936. AhcY. 1 hit.
PROSITEPS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB0R7F2_HALS3
AccessionPrimary (citable) accession number: B0R7F2
Entry history
Integrated into UniProtKB/TrEMBL: April 8, 2008
Last sequence update: April 8, 2008
Last modified: January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info