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B0R7C0

- ENDA_HALS3

UniProt

B0R7C0 - ENDA_HALS3

Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.UniRule annotation

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei277 – 2771UniRule annotation
    Active sitei288 – 2881UniRule annotation
    Active sitei319 – 3191UniRule annotation

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciHSAL478009:GJVF-1798-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
    Alternative name(s):
    tRNA-intron endonucleaseUniRule annotation
    Gene namesi
    Name:endAUniRule annotation
    Ordered Locus Names:OE_4102R
    OrganismiHalobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
    Taxonomic identifieri478009 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
    ProteomesiUP000001321: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 343343tRNA-splicing endonucleasePRO_1000188344Add
    BLAST

    Proteomic databases

    PRIDEiB0R7C0.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi478009.OE4102R.

    Structurei

    3D structure databases

    ProteinModelPortaliB0R7C0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000226561.
    KOiK01170.
    OMAiRGFYLSP.

    Family and domain databases

    Gene3Di3.40.1350.10. 2 hits.
    HAMAPiMF_01834. EndA_long.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 2 hits.
    PF02778. tRNA_int_endo_N. 2 hits.
    [Graphical view]
    SUPFAMiSSF53032. SSF53032. 2 hits.
    SSF55267. SSF55267. 2 hits.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B0R7C0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDGDLRGDTV HIGGDARQRF HDARGYGYPL GGNDIAVSLV EAAHLLFRGD    50
    LDSVDGMGFR AFLTDREAGF AARFLVYVDL RDRGFYLVPD RDPWWRDPGD 100
    GDFVVFPRGN TRRDGVVKHR VRVVDERTTL PVDGLSESVL AVVDEESEIT 150
    YLDIAPETPT GETTLDRPTD VPGVLLDDRV LVWEPPQRLH NAGFYGQPLG 200
    GRAADHDALQ LSLVEAAYLI DAGVLRLTDA TIDDVRARGR LGEGEHFDCR 250
    LAVYRALRDA GAVPKTGFKF GADFRVYSAV SSVDDLGHSE LLVRVITDTH 300
    VFSPGDLSLD VRLAHGVRKR MVFAATDDTD DTIRWLSVSR LTP 343
    Length:343
    Mass (Da):37,872
    Last modified:April 8, 2008 - v1
    Checksum:iE84404AC0C317CA8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM774415 Genomic DNA. Translation: CAP14639.1.
    RefSeqiYP_001689985.1. NC_010364.1.

    Genome annotation databases

    EnsemblBacteriaiCAP14639; CAP14639; OE_4102R.
    GeneIDi5954436.
    KEGGihsl:OE4102R.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM774415 Genomic DNA. Translation: CAP14639.1 .
    RefSeqi YP_001689985.1. NC_010364.1.

    3D structure databases

    ProteinModelPortali B0R7C0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 478009.OE4102R.

    Proteomic databases

    PRIDEi B0R7C0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAP14639 ; CAP14639 ; OE_4102R .
    GeneIDi 5954436.
    KEGGi hsl:OE4102R.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000226561.
    KOi K01170.
    OMAi RGFYLSP.

    Enzyme and pathway databases

    BioCyci HSAL478009:GJVF-1798-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1350.10. 2 hits.
    HAMAPi MF_01834. EndA_long.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 2 hits.
    PF02778. tRNA_int_endo_N. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53032. SSF53032. 2 hits.
    SSF55267. SSF55267. 2 hits.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1."
      Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D.
      Genomics 91:335-346(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29341 / DSM 671 / R1.

    Entry informationi

    Entry nameiENDA_HALS3
    AccessioniPrimary (citable) accession number: B0R7C0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3