Reviewed, UniProtKB/Swiss-Prot B0R6W9 (NADE_HALS3)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	OE3843F

Organism

Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) [Complete proteome] [HAMAP]

Taxonomic identifier

478009 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099024
Regions
	Nucleotide binding	45 – 52	8	ATP By similarity
Sites
	Active site	47	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0R6W9-1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: A774C8B25168FDA7	FASTA	268	28,408
	10 20 30 40 50 60 MSAPDTVSPL DLRFSAAELA ERRDRIQSFI RDTVAAAGAE RCVLGLSGGI DSTTVAHLTV 70 80 90 100 110 120 DELGADALHG LVMPGAVSRD QNMSDAERVA EDLGIEYDVV EIDPFVTQLT DVFPDAAGDE 130 140 150 160 170 180 VAVGNARART RAVINYFVAN HGDGVVLGTG NRAEAMTGYY TKYGDQAVDC NPIGNLYKMQ 190 200 210 220 230 240 VRQLARDLGV PEDLVTKAPT AELWADQTDA GELGVDYDTI DAVLAVHVDG GLPASATATH 250 260 LDIDPSVVET VRDLYGASKH KRAMPPAP

References

[1]

"Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346(2008) [PubMed: 18313895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM774415 Genomic DNA. Translation: CAP14488.1.
RefSeq	YP_001689834.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5954399.
GenomeReviews	Gene locus OE3843F in contig AM774415_GR.
KEGG	hsl:OE3843F.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LYAFAQK.
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_HALS3

Accession

Primary (citable) accession number: B0R6W9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)